Home Metabolism AdipoR agonist Schisandrin A For research use only.

Schisandrin A

Synonyms: Deoxyschizandrin, Wuweizisu A

Schisandrin A (Sch A, Deoxyschizandrin, Wuweizisu A) is an active component of Schisandrae Fructus with liver-protective, antitumor, and antioxidant activities. It is an agonist of the adiponectin receptor 2 (AdipoR2) with the IC50 value of 3.5 μM.

Schisandrin A Chemical Structure

Schisandrin A Chemical Structure

CAS No. 61281-38-7

Purity & Quality Control

Batch: S382201 DMSO]83 mg/mL]false]]]false]]]false Purity: 99.89%
99.89

Schisandrin A Related Products

Biological Activity

Description Schisandrin A (Sch A, Deoxyschizandrin, Wuweizisu A) is an active component of Schisandrae Fructus with liver-protective, antitumor, and antioxidant activities. It is an agonist of the adiponectin receptor 2 (AdipoR2) with the IC50 value of 3.5 μM.
Targets
AdipoR2 [4]
3.5 μM
In vitro
In vitro Schisandrin A significantly suppresses the lipopolysaccharide (LPS)-induced production of the key pro-inflammatory mediators nitric oxide (NO) and prostaglandin E2 by suppressing the expression of inducible NO synthase and cyclooxygenase-2 at the mRNA and protein levels in RAW 264.7 macrophages. It is demonstrated to reduce the LPS-induced secretion of pro-inflammatory cytokines, including tumor necrosis factor-α and interleukin-1β; this is accompanied by a simultaneous decrease in the respective mRNA and protein levels in the macrophages. In addition, the LPS- induced translocation of nuclear factor-κB (NF-κB), as well as activation of mitogen-activated protein kinases (MAPKs) and phosphatidylinositol-3 kinase (PI3K)/Akt pathways are inhibited by schisandrin A. These results suggest that schisandrin A has a protective effect against LPS-induced inflammatory and oxidative responses in RAW 264.7 cells by inhibiting the NF-κB, MAPK and PI3K/Akt pathways. Schisandrin A possesses anti-inflammatory activities and excellent Nrf2-induction or ROS-scavenging abilities[2]. Schisandrin A can inhibit the replication of four serotypes of DENV in a concentration- and time-dependent manner, with an effective half-maximal effective concentration 50% (EC50) value of 28.1 ± 0.42 μM against DENV serotype type 2 without significant cytotoxicity[3].
Cell Research Cell lines The RAW 264.7 murine macrophage cell line
Concentrations 0-200 μM
Incubation Time 1 h
Method

To evaluate the cytotoxicity of schisandrin A, RAW 264.7 cells are seeded in 96-well plates at a density of 1×103 cells/well. The cells are treated with various concentrations of schisandrin A for 1 h prior to incubation with LPS (100 ng/ml) for 24 h. After the incubation was complete, images of cells from each well are captured under a phase-contrast microscope. Subsequently, MTT is added to each well at 0.5 mg/ml, followed by incubation at 37°C in the dark. After 3 h of incubation, the MTT solution is removed and 200 μl 5% DMSO is added to dissolve the crystals. The viable cells are detected by reading the absorbance of formazan at 540 nm using an enzyme-linked immunosorbent assay (ELISA) microplate reader. The optical density of the formazan formed in the control (untreated) cells is considered to represent 100% viability.
In Vivo
In vivo Schisandrin A has proven beneficial in preventing cell damage in the pathogenesis of central nervous system diseases, including ischemia[2]. Schisandrin A can effectively protect mice from DENV (Dengue virus) infection by reducing disease symptoms and mortality of DENV-infected mice. It stimulates IFN-mediated antiviral responses in vivo[3].
Animal Research Animal Models Male Kunming White mice
Dosages 1, 10, 20 mg/kg d
Administration intragastrically administrated

Chemical Information & Solubility

Molecular Weight 416.51 Formula

C24H32O6
CAS No. 61281-38-7 SDF Download Schisandrin A SDF
Smiles CC1CC2=CC(=C(C(=C2C3=C(C(=C(C=C3CC1C)OC)OC)OC)OC)OC)OC
Storage (From the date of receipt)

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DMSO : 83 mg/mL ( (199.27 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)


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