Home Proteases Secretase inhibitor - Beta Amyloid inhibitor - Notch inhibitor RO4929097 For research use only.

RO4929097

Synonyms: RG-4733

RO4929097 (RG-4733) is a γ secretase inhibitor with IC50 of 4 nM in a cell-free assay, inhibiting cellular processing of Aβ40 and Notch with EC50 of 14 nM and 5 nM, respectively. Phase 2.

RO4929097 Chemical Structure

RO4929097 Chemical Structure

CAS No. 847925-91-1

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RO4929097 Related Products

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
U87 Cell Viability Assay 30 μM 4 d decreases cell viability 24495907
U87 R1 Cell Viability Assay 30 μM 4 d decreases cell viability 24495907
MGG4 Cell Viability Assay 30 μM 4 d decreases cell viability 24495907
MGG6 Cell Viability Assay 30 μM 4 d decreases cell viability 24495907
MGG8 Cell Viability Assay 30 μM 4 d decreases cell viability 24495907
MGG23 Cell Viability Assay 30 μM 4 d decreases cell viability 24495907
SUM149 Cell Viability Assay 0-10 μM 72 h inhibits cell growth dose dependently 22547109
Sum190 Cell Viability Assay 0-10 μM 72 h inhibits cell growth dose dependently 22547109
WM35 Function Assay 10 uM 24 h DMSO decreases the levels of NOTCH downstream target HES1 21980408
WM98.1 Function Assay 10 uM 24 h DMSO decreases the levels of NOTCH downstream target HES1 21980408
WM115 Function Assay 10 uM 24 h DMSO decreases the levels of NOTCH downstream target HES1 21980408
WM983A Function Assay 10 uM 24 h DMSO decreases the levels of NOTCH downstream target HES1 21980408
WM3248  Function Assay 10 uM 24 h DMSO decreases the levels of NOTCH downstream target HES1 21980408
WM35 Growth Inhibition Assay 10 uM 0-18 d DMSO inhibits cell growth time dependently 21980408
WM98.1 Growth Inhibition Assay 10 uM 0-18 d DMSO inhibits cell growth time dependently 21980408
WM115 Growth Inhibition Assay 10 uM 0-18 d DMSO inhibits cell growth time dependently 21980408
WM983A Growth Inhibition Assay 10 uM 0-18 d DMSO inhibits cell growth time dependently 21980408
WM3248  Growth Inhibition Assay 10 uM 0-18 d DMSO inhibits cell growth time dependently 21980408
A673 qHTS assay 29435139
DAOY qHTS assay 29435139
BT-37 qHTS assay 29435139
BT-12 qHTS assay 29435139
OHS-50 qHTS assay 29435139
SJ-GBM2 qHTS assay 29435139
SK-N-MC qHTS assay 29435139
NB-EBc1 qHTS assay 29435139
LAN-5 qHTS assay 29435139
Click to View More Cell Line Experimental Data

Biological Activity

Description RO4929097 (RG-4733) is a γ secretase inhibitor with IC50 of 4 nM in a cell-free assay, inhibiting cellular processing of Aβ40 and Notch with EC50 of 14 nM and 5 nM, respectively. Phase 2.
Targets
γ secretase [1]
(Cell-free assay)		 Notch [1]
(Cell-free assay)		 Aβ40 [1]
(Cell-free assay)
4 nM 5 nM 14 nM
In vitro
In vitro RO4929097 decreases the amount of Aβ peptides secreted into the culture medium in HEK293 cells with EC50 of 14 nM. RO4929097 strongly inhibits Notch processing with EC50 of 5 nM in the Notch cell-based reporter assay. The potency of RO4929097 in cell-free and cellular assays is in the low nanomolar range with >100-fold selectivity observed with respect to 75 other proteins of various types including receptors, ion channels, and enzymes (CEREP panel). After 5 days of treatment, RO4929097 reduces the production of ICN in the human NSCLC A549 cells inducing a flattened and less transformed tumor cell phenotype in tissue culture. [1] RO4929097 blocks Notch processing in human non-small cell lung carcinoma cells and decreases expression of the Notch transcriptional target gene Hes1. Treatment with RO4929097 reveals a two- to threefold decrease in the expression of direct Notch target genes, Hes1, Hey1, and Heyl in SUM149 and a 3.5- to eightfold decrease in expression in SUM190 cells. RO4929097 modestly inhibits the growth of SUM149 cells in a dose-dependent manner. At a concentration of 1 μM of RO4929097, growth inhibition is 20 % for SUM149 and 10 % for SUM190 cells, relative to vehicle-treated controls. RO4929097 decreases the production of inflammatory cytokines by T-cells. Furthermore, with RO4929097 treatment, there is a shift in favor of TH2 over TH1 cytokines. In addition, T-cell activation induced IL-6 production would be increased with RO4929097. [2] Upon RO4929097 treatment, the selected melanoma cell lines reveals downregulation of NOTCH downstream effector HES1. A decrease in the amount of melanospheres formed upon RO4929097 treatment in primary melanoma cell lines is detected. [3]
Kinase Assay In vitro potency assays
After RO4929097 is used, the Aβ peptides are measured by ECL assays using a variety of anti-Aβ antibodies and an Origen 1.5 Analyzer. The 4G8 murine mAb binds an epitope in the Aβ peptide (within amino acids 18–21) that is immediately distal to the α-secretase cleavage site. The G2–10 murine mAb binds the C terminus that is exposed after γ-secretase-mediated cleavage to generate amino acid 40 of the Aβ40 peptide. The FCA3542 rabbit antibody binds the C terminus that is exposed after γ-secretase-mediated cleavage to generate amino acid 42 of the Aβ42 peptide. The 4G8 mAb is biotinylated with biotin-LC-sulfo-N-hydroxysuccinimide-ester. The G2–10 and FCA3542 antibodies are ruthenylated with TAG-N-hydroxysuccinimide ester. Aβ(x-40) is detected with biotinylated 4G8 and ruthenylated G2–10. Aβ(x-42) is detected with biotinylated 4G8 and ruthenylated FCA3542.
Cell Research Cell lines WM35 and WM98.1 cell lines
Concentrations 10 μM
Incubation Time DMSO
Method Primary melanoma cell lines, including WM35 and WM98.1, are seeded at 2.5 × 103 cells per well on a 12-well dish in triplicate. The day after (day 0), the medium is replaced, and DMSO or 10 μM RO4929097 is added and changed every 3-4 days. At the indicated time points, cells are fixed in 10% formalin solution and stored in PBS at 4 °C. At day 18-24, all the plates are stained with crystal violet. After color elution with 10% acetic acid, optical density is read at 590 nm.
Experimental Result Images Methods Biomarkers Images PMID
Western blot Hey1 Snail / N-cadherin / Twist / E-cadherin Akt / p-Akt / Notch / IGF1R / FBXW7 NICD / Hes1 / Hes3 / Hes5 29899322
Growth inhibition assay Cell viability 30669546
In Vivo
In vivo Oral injection of 3 to 60 mg/kg RO4929097 once daily or twice daily to nude mice bearing A549 NSCLC xenografts for either 7, 14, or 21 days of a 21-day schedule results in significant tumor growth inhibition compared with vehicle-treated animals. The tumor growth inhibition values ranges from 66% to 91%. When mice are treated with 60 mg/kg RO4929097 twice daily with the 7+/14- schedule, treatment initially arouses regression of established A549 tumors. At the end of the 21-day cycle (day 47), tumor growth prevention is still 91% compared with vehicle control mice. Inhibition of tumor growth remains prolonged and sustained up to 34 days post-treatment (day 67). On day 67, these mice are retreated with the same dose of RO4929097 for a second cycle (7 days) until day 74. Importantly, the antitumor effects are sustained after dosing is completed. [1] RO4929097 leads to reduced expression of genes associated with angiogenesis in A549 xenograft model. In contrast, the RO4929097-resistant H460a xenograft displays little change in expression of these genes, underscoring the in vivo anti-angiogenesis mechanism of action of RO4929097.[2] For IL6 and IL8 overexpressing tumors, RO4929097 no longer impacts angiogenesis or the infiltration of tumor associated fibroblasts. [4]
Animal Research Animal Models Female nude mice bearing Calu-6 cells
Dosages 3 to 60 mg/kg
Administration Oral administration

Chemical Information & Solubility

Molecular Weight 469.4 Formula

C22H20F5N3O3
CAS No. 847925-91-1 SDF Download RO4929097 SDF
Smiles CC(C)(C(=O)NCC(C(F)(F)F)(F)F)C(=O)NC1C2=CC=CC=C2C3=CC=CC=C3NC1=O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 94 mg/mL ( (200.25 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 50 mg/mL

Water : Insoluble


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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Frequently Asked Questions

Question 1:
How about the half-life of RO4929097(S1575)?

Answer:
For S1575, the half-life is about 20 hours based on the following paper: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3869895/

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