Home Proteases Secretase inhibitor - Cysteine Protease inhibitor MDL-28170 For research use only.

MDL-28170

Synonyms: Calpain Inhibitor III

MDL-28170 (Calpain Inhibitor III) is a potent and selective calpain inhibitor of calpain that penetrates the blood-brain barrier and inhibits brain cysteine protease activity after systemic administration. MDL-28170 is also an inhibitor of γ-secretase.

MDL-28170 Chemical Structure

MDL-28170 Chemical Structure

CAS No. 88191-84-8

Purity & Quality Control

MDL-28170 Related Products

Biological Activity

Description MDL-28170 (Calpain Inhibitor III) is a potent and selective calpain inhibitor of calpain that penetrates the blood-brain barrier and inhibits brain cysteine protease activity after systemic administration. MDL-28170 is also an inhibitor of γ-secretase.
Targets
calpain [1] Secretase [2]
In vitro
In vitro

MDL28170 significantly enhances Schwann cells (SCs) survival in vitro in response to oxidative stress induced by application of H2O2, without reducing LDH release.[3]
Cell Research Cell lines Schwann cells
Concentrations 50 nM, 500 nM, 5 μM, 50 μM
Incubation Time 4 h
Method

To test the ability of MDL28170 to inhibit H2O2-mediated necrosis. Cells are treated with MDL28170 (50 nM, 500 nM, 5 μM, or 50 μM). After 1 h of drug pretreatment, fresh solution of drugs and H2O2 is added to the cells for the duration of the experiment. After 3 h of exposure the number of live or dead cells is assayed.
In Vivo
In vivo

MDL 28170 is a calpain inhibitor that protects against cortical neuronal damage even if the treatment is delayed until 3 h after reperfusion. However, the neuroprotective effect of this agent is less pronounced in the hippocampal CA1 sector.[1]
Animal Research Animal Models Male Mongolian gerbils
Dosages 50 mg/kg
Administration IP

Chemical Information & Solubility

Molecular Weight 382.45 Formula

C22H26N2O4
CAS No. 88191-84-8 SDF --
Density g/mL
Smiles CC(C)C(C(=O)NC(CC1=CC=CC=C1)C=O)NC(=O)OCC2=CC=CC=C2
Storage (From the date of receipt) 2 years -80 in solvent

In vitro
Batch:

DMSO : 76 mg/mL ( (198.71 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 10 mg/mL

Water : Insoluble


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In vivo
Batch:

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In vivo Formulation Calculator

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In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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