Home Transmembrane Transporters CD markers inhibitor SodiuM Metatungstate For research use only.

SodiuM Metatungstate

Synonyms: Sodium Polytungstate

Sodium metatungstate (SMT) solution is an inorganic heavy liquid which is widely used in density fractionation. Sodium metatungstate (Sodium polyoxotungstates) is a potent and selective inhibitor of ecto-nucleoside triphosphate diphosphohydrolase (NTPDases, CD39) with Ki of 0.140 μM, 0.910 μM and 0.563 μM for NTPDase1, NTPDase2 and NTPDase3, respectively.

SodiuM Metatungstate Chemical Structure

SodiuM Metatungstate Chemical Structure

CAS No. 12141-67-2

Purity & Quality Control

Batch: S552501 Water]50 mg/mL]false]DMSO]30 mg/mL]false]]]false Purity: 98%
98

SodiuM Metatungstate Related Products

Biological Activity

Description Sodium metatungstate (SMT) solution is an inorganic heavy liquid which is widely used in density fractionation. Sodium metatungstate (Sodium polyoxotungstates) is a potent and selective inhibitor of ecto-nucleoside triphosphate diphosphohydrolase (NTPDases, CD39) with Ki of 0.140 μM, 0.910 μM and 0.563 μM for NTPDase1, NTPDase2 and NTPDase3, respectively.
Targets
NTPDase1 [1]
(Cell-free assay)		 NTPDase3 [1]
(Cell-free assay)		 NTPDase2 [1]
(Cell-free assay)
0.140 μM(Ki) 0.563 μM(Ki) 0.910 μM(Ki)
In vitro
In vitro

Sodium metatungstate, a inhibitor of CD39, can partially reverse the suppression of CD8[+H] T cells induced by the presence of peritoneal macrophage.[2]
Cell Research Cell lines Peritoneal lavage cells, CD8+ T cells (purified from ACK-lysed splenocytes from C57BL/6 mice)
Concentrations 50 μM
Incubation Time 48 h
Method

Peritoneal lavage cells are plated in complete RPMI media on a 10 cm dish. Celexocib (10 μM), anti-TGF-b (clone 1D11, 10 μg/ml), or sodium metatungstate (50 μM) are plated onto respective wells of a 96 well round bottom plate. After at least five rounds of gentle aspiration and washing of the 10 cm plate containing the peritoneal lavage cells, adherent peritoneal macrophages are removed with a cell scraper, collected, and counted. 105 peritoneal macrophages are plated per indicated well of the 96 well plates. CD8+ T cells are purified from ACK-lysed splenocytes from C57BL/6 mice utilizing the Mouse CD8a+ T Cell Isolation Kit and then 105 purified CD8+ T cells are plated onto in T cell medium with 50 IU/ml murine IL-2. Certain wells are incubated with 105 anti-mouse CD3/CD28 Dynabeads for T cell activation. Flow cytometry is performed after 48 hours of culture.

Chemical Information & Solubility

Molecular Weight 2986.01 Formula

H2O40W12.6Na
CAS No. 12141-67-2 SDF --
Smiles O.[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[O-][W]([O-])(=O)=O.[O-][W]([O-])(=O)=O.[O-][W]([O-])(=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

Water : 50 mg/mL

DMSO : 30 mg/mL ( (10.04 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy SodiuM Metatungstate | SodiuM Metatungstate supplier | purchase SodiuM Metatungstate | SodiuM Metatungstate cost | SodiuM Metatungstate manufacturer | order SodiuM Metatungstate | SodiuM Metatungstate distributor