Home Apoptosis Pyroptosis activator Polyphyllin VI For research use only.

Polyphyllin VI

Polyphyllin VI derived from Paris polyphylla possess anti-cancer activities.

Polyphyllin VI Chemical Structure

Polyphyllin VI Chemical Structure

CAS No. 55916-51-3

Purity & Quality Control

Polyphyllin VI Related Products

Biological Activity

Description Polyphyllin VI derived from Paris polyphylla possess anti-cancer activities.
In vitro
In vitro

Polyphyllin VI (PPVI) induces activation of the NLRP3 inflammasome and exerts anti-proliferative effect via the induction of the ROS/NF-κB/NLRP3/GSDMD signal axis in non-small cell lung cancer (NSCLC).<sup><a class="sref"href="#s_ref">[1]</a></sup>
Cell Research Cell lines A549, H1299 cells
Concentrations 3-6 μM
Incubation Time 24 h, 48 h, 72 h
Method

A549 or H1299 cells seeded onto 96-well cell plates at a density of 5×103 cells/well are treated with PPVI under the indicated concentrations for 24, 48, and 72 h. After treatment, 10 μL of MTT solution (5 mg/mL) is added to each well and continued to incubate for another 4 h. The medium is then removed and 150 L of DMSO is added to each well to dissolve the formed formazan in the cells. After shaking at low speed for 10 min at room temperature, the colorimetric reading of the solute mixture is then determined by spectrophotometer at OD 570 nm.
In Vivo
In vivo

Polyphyllin VI (PPVI), a main saponin isolated from TTM, activates the NLRP3 inflammasome in A549-bearing athymic nude mice.<sup><a class="sref"href="#s_ref">[1]</a></sup>
Animal Research Animal Models A549-Bearing Athymic Nude Mice
Dosages 2.5, 5, and 10 mg/kg
Administration i.p.

Chemical Information & Solubility

Molecular Weight 738.90 Formula

C39H62O13
CAS No. 55916-51-3 SDF --
Smiles CC1CCC2(C(C3(C(O2)CC4C3(CCC5C4CC=C6C5(CCC(C6)OC7C(C(C(C(O7)CO)O)O)OC8C(C(C(C(O8)C)O)O)O)C)C)O)C)OC1
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 100 mg/mL ( (135.33 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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