Home ER stress & UPR HSP (HSP90) inhibitor Pseudolaric Acid A For research use only.

Pseudolaric Acid A

Pseudolaric acid A, a Hsp90 inhibitor with an IC50 of 0.60 μM, 2.72 μM, 1.36 μM, 2.92 μM and 6.16 μM in HL-60, A549, SMMC-7721, HeLa and SW480 cells respectively, is the main bioactive ingredient in Pseudolarix cortex, and induces cell cycle arrest at G2/M phase and promotes cell death through caspase-8/caspase-3 pathway, demonstrating potent antiproliferation and anticancer activities.

Pseudolaric Acid A Chemical Structure

Pseudolaric Acid A Chemical Structure

CAS No. 82508-32-5

Purity & Quality Control

Batch: E024901 DMSO]78 mg/mL]false]]]false]]]false Purity: 99.78%
99.78

Pseudolaric Acid A Related Products

Signaling Pathway

HSP (HSP90) Signaling Pathways

HSP (HSP90) Signaling Pathway

Biological Activity

Description Pseudolaric acid A, a Hsp90 inhibitor with an IC50 of 0.60 μM, 2.72 μM, 1.36 μM, 2.92 μM and 6.16 μM in HL-60, A549, SMMC-7721, HeLa and SW480 cells respectively, is the main bioactive ingredient in Pseudolarix cortex, and induces cell cycle arrest at G2/M phase and promotes cell death through caspase-8/caspase-3 pathway, demonstrating potent antiproliferation and anticancer activities.
Targets
Hsp90 [1]
(in HL-60 cells cytotoxicity assay)		 Hsp90 [1]
(in SMMC-7721 cells cytotoxicity assay)		 Hsp90 [1]
(in A549 cells cytotoxicity assay)		 Hsp90 [1]
(in HeLa cells cytotoxicity assay)		 Hsp90 [1]
(in SW480 cells cytotoxicity assay)
0.60 μM 1.36 μM 2.72 μM 2.92 μM 6.16 μM
In vitro
In vitro

Pseudolaric Acid A is more sensitive to leukemia P-388 and melanoma SK-MEL5, suggesting it deserves further development as potentially useful antitumor agent.[2]
Cell Research Cell lines KB, A-549, HCT-8, P-388, and L-1210 tumor cells
Concentrations --
Incubation Time --
Method

KB screen: The KB cells are maintained on Roswell Park Memorial Institute (RMPI) 1640 media supplemented with 5 % fetal calf serum and 100 µg/ml kanamycin. The cells (5 x 10<sup>4</sup>cell/ml) are plated 24 hours before the addition of Pseudolaric Acid A. All counting of KB cells is done on a hemacytometer. Results are expressed as the dose that inhibits growth to 50 % of control growth by three days after drug addition. P-388 and L-1210 screens: The P-388 lymphocytic leukemia and L- 1210 lymphoid leukemia cells are maintained in minimum essential medium (MEM) plus 10% fetal calf serum and 100 µg/ml penicillin/ streptomycin. Cells (10<sup>4</sup>) are incubated with Pseudolaric Acid A prepared in 0.05 % tween-80 NaCI. Cells are counted in control and treated tubes on day 3 using a hemacytometer. A-549, HCT-8 screens: Media used for the propagation and growth of these cell lines are F-12 with 10% fetal calf serum (FCS) for A-549, RPMI-1640 with sodium pyruvate and 10 % horse serum for HCT- 8. All media are treated with 50 ng/ml of gentocin to prevent bacterial contamination. The cells are grown in a humidified atmosphere of 5% CO2 and 95% air at 37℃. 24-well tissue culture plates are seeded each well with approximately 1 x 10<sup>4</sup>cells per 2 ml of growth medium and incubated at 37℃ overnight. The starting cell numbers in quadruplicate wells are determined by dispersing the monolayer with trypsin-EDTA solution and the total number of cells per well is determined. Pseudolaric Acid A in suitable concentration are added to quadruplicate wells in order to achieve a maximum treatment dosage of 10 µg/ml. After further incubation of the tissue culture plates at 37℃ for 72 h, the cell numbers in each well are determined.

Chemical Information & Solubility

Molecular Weight 388.45 Formula

C22H28O6
CAS No. 82508-32-5 SDF --
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 78 mg/mL ( (200.79 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)


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