Home MAPK S6 Kinase inhibitor - p38 MAPK inhibitor - ERK activator - JNK activator 3'-Hydroxypterostilbene For research use only.

3'-Hydroxypterostilbene

Synonyms: 3'-HPT

3'-Hydroxypterostilbene (3'-HPT) is one of the active constituents of Sphaerophysa salsula and Pterocarpus marsupium which may be useful in treating different types of haematological malignancies. 3'-Hydroxypterostilbene, a natural pterostilbene analogue, effectively inhibits the growth of human colon cancer cells (IC50s of 9.0, 40.2, and 70.9 µM for COLO 205, HCT-116, and HT-29 cells, respectively) by inducing apoptosis and autophagy. 3'-Hydroxypterostilbene inhibits the PI3K/Akt/mTOR/p70S6K, and p38MAPK pathways and activates the ERK1/2, JNK1/2 MAPK pathways.

3'-Hydroxypterostilbene Chemical Structure

3'-Hydroxypterostilbene Chemical Structure

CAS No. 475231-21-1

Purity & Quality Control

Batch: S394001 DMSO]54 mg/mL]false]Ethanol]54 mg/mL]false]Water]Insoluble]false Purity: 99.55%
99.55

3'-Hydroxypterostilbene Related Products

Signaling Pathway

S6 Kinase Signaling Pathways

S6 Kinase Signaling Pathway

Biological Activity

Description 3'-Hydroxypterostilbene (3'-HPT) is one of the active constituents of Sphaerophysa salsula and Pterocarpus marsupium which may be useful in treating different types of haematological malignancies. 3'-Hydroxypterostilbene, a natural pterostilbene analogue, effectively inhibits the growth of human colon cancer cells (IC50s of 9.0, 40.2, and 70.9 µM for COLO 205, HCT-116, and HT-29 cells, respectively) by inducing apoptosis and autophagy. 3'-Hydroxypterostilbene inhibits the PI3K/Akt/mTOR/p70S6K, and p38MAPK pathways and activates the ERK1/2, JNK1/2 MAPK pathways.
Targets
p70S6K [1] p38MAPK [1]
In vitro
In vitro 3'-Hydroxypterostilbene decreases cell growth in cultured human colon cancer cells (COLO 205, HCT-116, and HT-29) in a dose-dependent manner, with IC50 values of 9.0, 40.2, and 70.9 µM, respectively. 3'-Hydroxypterostilbene effectively inhibits the growth of human colon cancer cells by inducing apoptosis and autophagy. Treatment with HPSB causes reduction of mitochondrial membrane potential and induction of caspase-3 and caspage-9, which is associated with the degradation of DFF-45 and PARP, precedes the onset of apoptosis. It significantly down-regulates phosphatidylinositol 3-kinase (PI3K)/Akt and mitogen-activated protein kinases (MAPKs) signalings including decreased the phosphorylation of mammalian target of rapamycin (mTOR)[1].
Cell Research Cell lines COLO 205 cells
Concentrations 5-100 µM
Incubation Time 24 h
Method

For sub-G1 cell population analysis, COLO 205 cells (2×105 cells/mL) are cultured in 12 well and treatment with various concentrations of pterostilbene or 3′-hydroxypterostilbene for 24 h. The cells are then harvested, washed with PBS, resuspended in 200 µL of PBS, and fixed in 800 µL of iced 100% ethanol at -20°C. After being left to stand overnight, the cell pellets are collected by centrifugation, resuspended in 1 mL of hypotonic buffer (0.5% Triton X-100 in PBS and 0.5 µg/mL RNase) with PI (50 µg/mL) and incubated at 37°C in the dark for 30 min. Fluorescence emitted from the PI-DNA complex is quantitated after excitation of the fluorescent dye.
In Vivo
In vivo 3'-Hydroxypterostilbene by i.p. injection has anti-tumor efficacy gainst colon cancer through the inhibition of inflammation, metastasis, and angiogenesis as well as through the induction of apoptosis[1].
Animal Research Animal Models nude mice bearing COLO 205 tumor xenografts
Dosages 10 mg/kg
Administration i.p.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT01199250 Not yet recruiting
Lynch Syndrome|Recurrent Uterine Corpus Carcinoma|Stage I Uterine Corpus Cancer|Stage II Uterine Corpus Cancer|Stage III Uterine Corpus Cancer|Stage IV Uterine Corpus Cancer
Gynecologic Oncology Group|National Cancer Institute (NCI)|GOG Foundation
 January 2100 --
NCT05454306 Suspended
Clavicle Fracture
University of Minnesota
 January 9 2028 Not Applicable
NCT05118594 Not yet recruiting
Major Depressive Disorder|Dysthymic Disorder|Specific Phobia|Social Anxiety Disorder|Panic Disorder|Agoraphobia|Generalized Anxiety Disorder
National Council of Scientific and Technical Research Argentina
 February 2026 Not Applicable
NCT06077526 Not yet recruiting
Chronic Pain
Bridgewater College
 January 13 2026 Not Applicable

Chemical Information & Solubility

Molecular Weight 272.30 Formula

C16H16O4
CAS No. 475231-21-1 SDF Download 3'-Hydroxypterostilbene SDF
Smiles COC1=CC(=CC(=C1)C=CC2=CC(=C(C=C2)O)O)OC
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 54 mg/mL ( (198.31 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 54 mg/mL

Water : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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