Home DNA Damage/DNA Repair RAD51 activator - CRISPR/Cas9 activator RS-1 For research use only.

RS-1

RS-1 is a RAD51-stimulatory compound, which increases the DNA binding activity of RAD51. It is an HDR(homology-directed repair) enhancer that enhances Cas9- and TALEN-mediated knock-in efficiency in rabbit embryos both in vitro and in vivo.

RS-1 Chemical Structure

RS-1 Chemical Structure

CAS No. 312756-74-4

Purity & Quality Control

Batch: S823401 DMSO]100 mg/mL]false]Water]Insoluble]false]Ethanol]Insoluble]false Purity: 99.30%
99.30

RS-1 Related Products

Biological Activity

Description RS-1 is a RAD51-stimulatory compound, which increases the DNA binding activity of RAD51. It is an HDR(homology-directed repair) enhancer that enhances Cas9- and TALEN-mediated knock-in efficiency in rabbit embryos both in vitro and in vivo.
Targets
RAD51 [1]
In vitro
In vitro

RS-1 stimulates the binding of hRAD51 to ssDNA. Low-micromolar concentrations of this small molecule enhance DNA binding and result in longer protein–DNA complex lengths. In addition, RS-1 stabilizes the active form of hRAD51 filaments and this is reflected in an enhanced strand assimilation activity[1]. RS-1 enhances Cas9- and TALEN-mediated knock-in efficiency in rabbit embryos both in vitro and in vivo[2].
Cell Research Cell lines Early-passage neonatal human dermal fibroblasts
Concentrations 1-7.5 μM
Incubation Time 24 h
Method

Early-passage neonatal human dermal fibroblasts were maintained in Medium 106 supplemented with low serum growth supplement. Cell-survival experiments were performed using a sulforhodamine B assay. Sulforhodamine B-stained cells are quantified with a Synergy plate reader.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT06066008 Recruiting
X-linked Retinoschisis
Zhongmou Therapeutics
 September 25 2022 Early Phase 1

Chemical Information & Solubility

Molecular Weight 524.23 Formula

C20H16Br2N2O3S
CAS No. 312756-74-4 SDF Download RS-1 SDF
Smiles C1=CC=C(C=C1)CNS(=O)(=O)C2=C(C=CC(=C2)C(=O)NC3=CC=C(C=C3)Br)Br
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 100 mg/mL ( (190.75 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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