Home GPCR & G Protein Guanylate Cyclase inhibitor ODQ For research use only.

ODQ

Synonyms: 1H-ODQ

ODQ (1H-ODQ) is a potent and selective inhibitor of soluble guanylyl cyclase (sGC, nitric oxide-activated enzyme) that enhances the pro-apoptotic effects of cisplatin in human mesothelioma cells.

ODQ Chemical Structure

ODQ Chemical Structure

CAS No. 41443-28-1

Purity & Quality Control

Batch: S979601 DMSO]37 mg/mL]false]Water]Insoluble]false]Ethanol]Insoluble]false Purity: 99.63%
99.63

ODQ Related Products

Biological Activity

Description ODQ (1H-ODQ) is a potent and selective inhibitor of soluble guanylyl cyclase (sGC, nitric oxide-activated enzyme) that enhances the pro-apoptotic effects of cisplatin in human mesothelioma cells.
Targets
sGC [1]
In vitro
In vitro

In vitro, administration of H2O2 (for 4 hrs) to rat cardiomyoblasts decreases mitochondrial respiration attributable to generation of hydroxyl radicals. Pretreatment of cells with ODQ does not abolish this cell injury. In addition, ODQ does not scavenge superoxide anions.[1]
Cell Research Cell lines H9c2 cells
Concentrations 0.1 μM to 1 mM
Incubation Time 2 hrs
Method

H2O2 is a useful tool for studying the effects of reactive oxygen species on different organs. It can readily diffuse across cell membranes and can be converted into the toxic hydroxyl radical at intracellular sites via the Fenton reaction in the presence of transition metals. Cells were preincubated (2 hrs, 37°C) with ODQ (0.1 mM to 1 mM), saline, or DMSO (media containing 10% DMSO). The cells then are exposed to media or H2O2 (1 mM for 4 hrs at 37°C) after which time cell injury is assessed. All experiments are carried out in duplicate of n = 4 observations.
In Vivo
In vivo

Pretreatment of rats with ODQ attenuates the renal dysfunction, lung injury, and hepatocellular injury caused by lipoteichoic acid/peptidoglycan or lipopolysaccharide.[1]
Animal Research Animal Models male Wistar rats
Dosages 2 mg/kg
Administration IP
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05535270 Completed
Mood Disorders|Emotional Blunting
First Affiliated Hospital of Zhejiang University
 January 1 2022 --
NCT03835715 Completed
Major Depressive Disorder
H. Lundbeck A/S
 February 5 2019 Phase 4

Chemical Information & Solubility

Molecular Weight 187.15 Formula

C9H5N3O2
CAS No. 41443-28-1 SDF --
Smiles O=C1ON=C2C=NC3=C(C=CC=C3)N12
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 37 mg/mL ( (197.7 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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