NSC622608

Catalog No.S9869 Purity:99.79%

NSC622608 is a first small-molecule ligand for V-domain Ig Suppressor of T-cell Activation (VISTA) with an IC50 of 4.8 μM in TR-FRET assay.

NSC622608 Chemical Structure

CAS No. 2593254-90-9

Purity & Quality Control

Batch: S986901 DMSO]64 mg/mL]false]Water]5 mg/mL]false]Ethanol]Insoluble]false Purity: 99.79%

99.79

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Related Compound Libraries	FDA-approved Drug Library Natural Product Library Bioactive Compound Library-I Express-Pick Library	Click to Expand

Biological Activity

Description

NSC622608 is a first small-molecule ligand for V-domain Ig Suppressor of T-cell Activation (VISTA) with an IC50 of 4.8 μM in TR-FRET assay.

Targets

VISTA ^[1]
(in TR-FRET assay)

4.8 μM

In vitro
In vitro	NSC622608 (VRC2) increases the expression of p53 in a dose- and time-dependent manner in wild-type p53-expressing MM cell lines, and induces the expression of p53 target genes P21, PUMA and NOXA to overcome proteasome inhibitors (PI) resistance.<sup><a class="sref" href="#s_ref">[1]</a></sup>
Cell Research	Cell lines	Wild-type p53 expressing NCI-H929 cells
	Concentrations	500 nM
	Incubation Time	2, 4, 6 and 8 h
	Method	Wild-type p53 expressing NCI-H929 cells were treated with 500 nM VRC2 for the indicated time points for western blots.

References
[1] Moustafa T Gabr, et al. J Am Chem Soc. 2020 Sep 23;142(38):16194-16198.

References

[1] Moustafa T Gabr, et al. J Am Chem Soc. 2020 Sep 23;142(38):16194-16198.

Chemical Information & Solubility

Molecular Weight	322.25	Formula	C₉H₁₂BrN₃OS₂
CAS No.	2593254-90-9	SDF	--
Smiles	Br.CN(C)C1=NC(SS1)=NCC2=CC=CO2
Storage (From the date of receipt)	3 years -20°C powder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years -20°C powder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 64 mg/mL ( (198.6 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 5 mg/mL

Ethanol : Insoluble

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In vivo
Batch:

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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