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L-Citrulline

L-citrulline is a substance called a non-essential amino acid that is used as a sports performance and cardiovascular health supplement.

L-Citrulline Chemical Structure

L-Citrulline Chemical Structure

CAS No. 372-75-8

Purity & Quality Control

L-Citrulline Related Products

Biological Activity

Description L-citrulline is a substance called a non-essential amino acid that is used as a sports performance and cardiovascular health supplement.
In vitro
In vitro L-Citrulline restores nitric oxide/reactive oxygen species balance and barrier function in high glucose-treated monolayers of human glomerular endothelial cells. Moreover, L-Citrulline also has the ability to establish an anti-inflammatory profile, characterized by increased IL-10 and reduced IL-1β and IL-12(p70) generation in the human proximal tubular cells[1].
Cell Research Cell lines Human glomerular endothelial cells
Concentrations 1 mM
Incubation Time 2 h
Method Human glomerular endothelial cell are seeded at 1 × 105 cells per well in 24-well plates. Confluent quiescent cell monolayers are exposed to HG or proper iso-osmotic control for 24 h. L-Citrulline (1 mM) is applied 2 h prior to HG. Exposure is terminated by removal of the supernatant. Fresh basic CSC medium is replaced and cells incubated for additional 30 min. Supernatant is then removed, subsequently centrifuged and stored at −80°C for NO analysis.
In Vivo
In vivo Oral l-cit (precursor of l-arg) augments NO-dependent signaling, not only by means of increasing l-arg synthesis, but also by decreasing l-arg catabolism, as such increasing circulating l-arg levels. L-Citrulline exerts protective effects in kidneys of STZ-rats, and markedly reduces urinary albumin excretion, tubulo-interstitial fibrosis, and kidney hypertrophy, observed in untreated diabetic mice[1]. L-citrulline supplementation also strengthens vascular endothelium function and attenuates stress-induced cold hypersensitivity by improving blood circulation[2].
Animal Research Animal Models diabetic mice (C57BL/6 mice, intraperitoneal injections of STZ)
Dosages 50 mg/kg
Administration oral
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT06207461 Not yet recruiting
Traditional Chinese Acupoint Message|Intestinal Barrier|Gastrointestinal Dysfunction
Wuhan Union Hospital China
 February 15 2024 --
NCT05636397 Recruiting
BPD - Bronchopulmonary Dysplasia|Pulmonary Hypertension|NEC
The Hospital for Sick Children
 November 1 2023 Not Applicable
NCT06092476 Not yet recruiting
Diabetes Mellitus Type 2
Academisch Medisch Centrum - Universiteit van Amsterdam (AMC-UvA)|Fractyl Health Inc.
 November 1 2023 Not Applicable
NCT06025383 Recruiting
Menopause|Hypertension
Texas Tech University
 July 20 2023 Not Applicable
NCT05590117 Enrolling by invitation
Colo-rectal Cancer|Neuropathy;Peripheral|Mucositis
Tanta University
 October 11 2022 Early Phase 1

Chemical Information & Solubility

Molecular Weight 175.19 Formula

C6H13N3O3
CAS No. 372-75-8 SDF Download L-Citrulline SDF
Smiles C(CC(C(=O)O)N)CNC(=O)N
Storage (From the date of receipt)

In vitro
Batch:

Water : 35 mg/mL

DMSO : Insoluble ( Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : Insoluble


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In vivo
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