Home Transmembrane Transporters Potassium Channel inhibitor JNJ 303 For research use only.

JNJ 303

JNJ 303 is a potent blocker of the voltage dependent IKs-channel encoded by KCNQ1/KCNE1 with IC50 of 64 nM. JNJ 303 induces QT-prolongations and causes unprovoked torsades de pointes (TdP).

JNJ 303 Chemical Structure

JNJ 303 Chemical Structure

CAS No. 878489-28-2

Purity & Quality Control

Batch: S008701 DMSO]88 mg/mL]false]Water]Insoluble]false]Ethanol]Insoluble]false Purity: 99.5%
99.5

JNJ 303 Related Products

Signaling Pathway

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Potassium Channel Signaling Pathway

Biological Activity

Description JNJ 303 is a potent blocker of the voltage dependent IKs-channel encoded by KCNQ1/KCNE1 with IC50 of 64 nM. JNJ 303 induces QT-prolongations and causes unprovoked torsades de pointes (TdP).
Targets
IKs-channel [1]
(Cell-free assay)
64 nM
In vitro
In vitro

JNJ 303 induces a decrease in ICaL, which is more pronounced in atrial cells compared to ventricular cells.[1]
Cell Research Cell lines murine atrial and ventricular cells
Concentrations 1 μM, 70 nM
Incubation Time --
Method

The cells were incubated with 10 μM Isoproterenol (ISO), dissolved in IMDM with 20 % FBS, at 37℃ for 1-2 hours,  prior to the measurement of APs in the whole cell mode. For recording of the ICaL, β-adrenergic stimulation was accomplished via perfusion of the cells with external solution containing 10 μM ISO. Further drug administration (JNJ 303) was done via the gravitational perfusion system. APs and currents were recorded in whole cell mode.

Chemical Information & Solubility

Molecular Weight 440.98 Formula

C21H29ClN2O4S
CAS No. 878489-28-2 SDF --
Smiles CC(C)(C(=O)NC1C2CC3CC1CC(C3)(C2)NS(=O)(=O)C)OC4=CC=C(C=C4)Cl
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 88 mg/mL ( (199.55 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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