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isoleucine

Synonyms: Ile

Isoleucine (Ile) is a non-polar, uncharged (at physiological pH), aliphatic amino acid that is used in the biosynthesis of proteins.

isoleucine Chemical Structure

isoleucine Chemical Structure

CAS No. 73-32-5

Purity & Quality Control

Batch: S375201 Water]26 mg/mL]false]DMSO]Insoluble]false]]]false Purity: 98%
98

isoleucine Related Products

Biological Activity

Description Isoleucine (Ile) is a non-polar, uncharged (at physiological pH), aliphatic amino acid that is used in the biosynthesis of proteins.
In vitro
In vitro Isoleucine plays a major role in enhancing glucose consumption and utilization by up-regulating intestinal and muscular glucose transporters[1]. L-isoleucine could induce β-defensins’expressions in porcine intestinal epithelial cells[2].
Cell Research Cell lines IPEC-J2 cells
Concentrations 0, 25, 50, 100, 250 and 500 μg/mL
Incubation Time 12 h
Method After cells are grown to 80 % confluence, they are starved for 12 h in serum and antibiotic-free DMEM/F12 medium. Following starvation, the cells are cultured for 12 h in the presence of 0, 25, 50, 100, 250 and 500 μg/mL L-isoleucine or 0, 25, 50, 100, 250 and 500 μmol/mL zinc sulfate that were used to increase the concentration of Zn2+ in the medium. Then, the cells are collected to examine the mRNA and protein expressions of pBD-1, 2 and 3, respectively.
In Vivo
In vivo Isoleucine is beneficial for mammary health, milk quality and embryo growth, enhances intestinal development, intestinal amino acid transportation and mucin production and participates in up-regulating innate and adaptive immune responses. Studies in mice have indicated that diet-induced obesity mice in an isoleucine treatment (final concentration of 2.5% isoleucine in drinking water) had almost 6% lower body weight gain and 49% less epididymal white adipose tissue mass compared with the control treatment, with higher levels of hepatic protein. Its deprivation induces fat mass loss in mice. Isoleucine could stimulate glucose uptake and participated in plasma glucose uptake in the rat[1].
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT06365268 Not yet recruiting
Perinatal Solid Tumors of the Thoraco-abdomino-pelvic Region
Assistance Publique - Hôpitaux de Paris
 May 2024 --
NCT06142513 Not yet recruiting
Obesity Hypoventilation Syndrome|Obesity|Hypoventilation|Respiration Disorders|Sleep Disorder; Breathing-Related
Istanbul University|Istanbul University - Cerrahpasa (IUC)
 April 10 2024 --
NCT05026047 Not yet recruiting
Papillomavirus Infection
Assistance Publique - Hôpitaux de Paris
 May 2023 Not Applicable
NCT05812144 Recruiting
Type 1 Facioscapulohumeral Muscular Dystrophy
Centre Hospitalier Universitaire de Nice
 May 23 2023 Not Applicable

Chemical Information & Solubility

Molecular Weight 131.17 Formula

C6H13NO2
CAS No. 73-32-5 SDF Download isoleucine SDF Download isoleucine SDF
Smiles CCC(C)C(C(=O)O)N
Storage (From the date of receipt)

In vitro
Batch:

Water : 26 mg/mL

DMSO : Insoluble ( Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)


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In vivo
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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