Home Microbiology CMV inhibitor Ganciclovir For research use only.

Ganciclovir

Synonyms: RS-21592, BW-759, 2'-Nor-2'-deoxyguanosine

Ganciclovir is an antiviral drug for feline herpesvirus type-1 with IC50 of 5.2 μM in a cell-free assay.

Ganciclovir Chemical Structure

Ganciclovir Chemical Structure

CAS No. 82410-32-0

Purity & Quality Control

Ganciclovir Related Products

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
E6SM cell lines Function assay Effective concentration required to inhibit Herpes simplex virus-2 (HSV-2) induced cytopathicity by 50% in E6SM cell lines, EC50=1.2 nM 11495586
OST TK- cells Cytotoxicity assay Cytotoxicity against human OST TK- cells expressing HSV1 TK, IC50=0.0019μM. 17181158
HEL Antiviral assay Antiviral activity against HSV2 G in HEL cells assessed as reduction of virus-induced cytopathogenicity, MIC=0.0064μM. 17672445
HEL Antiviral assay Antiviral activity against HSV1 KOS infected in HEL cells assessed as inhibition of virus-induced cytopathic effect, EC50=0.01μM. 21232828
HEL Antiviral assay Antiviral activity against HSV-2 G infected in HEL cells assessed as inhibition of viral-induced cytopathogenicity, EC50=0.01μM. 23795238
HEL Antiviral assay Antiviral activity against HSV-1 KOS infected in HEL cells assessed as inhibition of viral-induced cytopathogenicity, EC50=0.01μM. 23795238
HEL Antiviral assay 3 days Antiviral activity against Herpes simplex virus 1 KOS infected in human HEL cells assessed as inhibition of virus-induced cytopathic effect after 3 days by microscopic method, EC50=0.01μM. 30286952
HEL Antiviral assay 3 days Antiviral activity against Human herpesvirus 2 strain G infected in human HEL cells assessed as inhibition of virus-induced cytopathic effect after 3 days by microscopic method, EC50=0.01μM. 30286952
HEL Antiviral assay Antiviral activity against HSV2 G infected in HEL cells assessed as inhibition of virus-induced cytopathic effect, EC50=0.013μM. 23099097
HEL Antiviral assay Antiviral activity against HSV1 KOS infected in HEL cells assessed as inhibition of virus-induced cytopathic effect, EC50=0.015μM. 23099097
OST TK- cells Cytotoxicity assay Cytotoxicity against human OST TK- cells expressing HSV1 TK in presence of 2.5 uM 1-[6-(triphenylmethoxy)hexyl]thymine, IC50=0.018μM. 17181158
HEL Antiviral assay Antiviral activity against HSV1 KOS in HEL cells, EC50=0.019μM. 17181162
HEL Antiviral assay 2 to 3 days Antiviral activity against Herpes simplex virus 2 G infected in HEL cells assessed as inhibition of plaque formation measured after 2 to 3 days post infection, EC50=0.019μM. 29550734
HEL Antiviral assay Antiviral activity against HSV1 KOS in human HEL cells assessed as protection against virus-induced cytopathicity, EC50=0.02μM. 19281225
HEL Antiviral assay 4 days Antiviral activity against HSV1 KOS infected in HEL cells assessed as inhibition of virus-induced cytopathogenicity after 4 days, EC50=0.02μM. 22459876
HEL Antiviral assay Antiviral activity against Herpes simplex virus 1 KOS infected in HEL cells assessed as reduction of virus-induced cytopathogenicity, EC50=0.02μM. 26001344
HEL Antiviral assay Antiviral activity against Herpes simplex virus 1 KOS infected in HEL cells assessed as inhibition of virus-induced cytopathicity, EC50=0.02μM. 29880251
HEL Antiviral assay Antiviral activity against Herpes simplex virus 2 G infected in HEL cells assessed as inhibition of virus-induced cytopathicity, EC50=0.02μM. 29880251
Vero Antiviral assay 72 hrs Antiviral activity against HSV-1 infected in African green monkey Vero cells assessed as inhibition of GFP expression after 72 hrs by GFP-based fluorescence microscopic analysis, EC50=0.025μM. 31503476
HEL Antiviral assay Antiviral activity against Human herpesvirus 2 G infected in HEL cells assessed as protection against virus-induced cytopathicity, EC50=0.027μM. 21565516
HEL Antiviral assay Antiviral activity against HSV1 KOS infected in HEL cells by viral CPE assay, EC50=0.027μM. 22858222
HEL Antiviral assay Antiviral activity against HSV2 G infected in HEL cells by viral CPE assay, EC50=0.029μM. 22858222
HEL Antiviral assay 2 to 3 days Antiviral activity against acyclovir-sensitive Herpes simplex virus 1 KOS infected in HEL cells assessed as protection from virus-induced cytopathogenicity after 2 to 3 days by MTT assay, EC50=0.03μM. 20034711
HEL Antiviral assay 2 to 3 days Antiviral activity against Herpes simplex virus 1 KOS infected in HEL cells assessed as protection from virus-induced cytopathogenicity after 2 to 3 days by MTT assay, EC50=0.03μM. 20034711
HEL Antiviral assay 2 to 3 days Antiviral activity against Herpes simplex virus 2 G infected in HEL cells assessed as protection from virus-induced cytopathogenicity after 2 to 3 days by MTT assay, EC50=0.03μM. 20034711
HEL Antiviral assay 4 days Antiviral activity against Herpes simplex virus 1 KOS infected in human HEL cells assessed as inhibition of virus-induced cytopathic effect after 4 days by microscopic analysis, EC50=0.03μM. 21696963
HEL Antiviral assay Antiviral activity against Human herpesvirus 1 KOS infected in HEL cells assessed as protection against virus-induced cytopathicity, EC50=0.03μM. 21565516
HEL Antiviral assay 4 days Antiviral activity against Herpes simplex virus-2 G infected in human HEL cells assessed as inhibition of virus-induced cytopathic effect after 4 days by microscopic analysis, EC50=0.03μM. 21696963
HEL Antiviral assay 4 days Antiviral activity against HSV2 G infected in HEL cells assessed as inhibition of virus-induced cytopathogenicity after 4 days, EC50=0.03μM. 22459876
HEL Antiviral assay Antiviral activity against Herpes simplex virus-2 G infected in human HEL cells assessed as reduction of virus-induced cytopathogenicity, EC50=0.03μM. 23811093
HEL Antiviral assay Antiviral activity against Herpes simplex virus-1 KOS infected in human HEL cells assessed as reduction of virus-induced cytopathogenicity, EC50=0.03μM. 23811093
HEL Antiviral assay Antiviral activity against Herpes simplex virus 2 G infected in HEL cells assessed as reduction of virus-induced cytopathogenicity, EC50=0.03μM. 26001344
HEL Antiviral assay Antiviral activity against Herpes simplex virus 2 G infected in HEL cells assessed as inhibition of virus plaque formation, EC50=0.03μM. 27639368
HELF Antiviral assay Antiviral activity against Human herpesvirus 1 KOS strain infected in HELF cells assessed as inhibition of virus-induced cytopathogenicity, EC50=0.03μM. 30098481
HELF Antiviral assay Antiviral activity against Human herpesvirus 2 G strain infected in HELF cells assessed as inhibition of virus-induced cytopathogenicity, EC50=0.03μM. 30098481
HEL Antiviral assay Antiviral activity against Herpes simplex virus 1 KOS infected in human HEL cells assessed as inhibition of virus-induced cytopathic effect, EC50=0.03μM. 28829913
HEL Antiviral assay Antiviral activity against Herpes simplex virus 2 G infected in HEL cells assessed as inhibition of virus-induced cytopathic effect, EC50=0.03μM. 28757102
HEL Antiviral assay Antiviral activity against HSV2 in HEL cells, EC50=0.032μM. 17181162
HEL Antiviral assay Antiviral activity against HSV1 KOS in HEL cells assessed as reduction of virus-induced cytopathogenicity, MIC=0.032μM. 17672445
HEL Antiviral assay Antiviral activity against Herpes simplex virus 1 KOS infected in HEL cells assessed as reduction in virus-induced cytopathogenicity, EC50=0.032μM. 27750154
OST TK- cells Cytotoxicity assay Cytotoxicity against human OST TK- cells expressing HSV1 TK in presence of 5 uM 1-[6-(triphenylmethoxy)hexyl]thymine, IC50=0.033μM. 17181158
fibroblast cells Antiviral assay Antiviral activity against Human cytomegalovirus AD169 infected in human foreskin fibroblast cells assessed as inhibition of virus-induced cytopathic effect, EC50=0.04μM. 19397271
HEL Antiviral assay Antiviral activity against HSV2 G infected in HEL cells assessed as inhibition of virus-induced cytopathic effect, EC50=0.04μM. 21232828
HFF Antiviral assay 1 hr Antiviral activity against Human cytomegalovirus (strain AD169) infected in 1 hr pretreated human HFF cells assessed as reduction in viral-induced cytopathic effect by neutral red uptake assay, EC50=0.04μM. 21376429
HEL Antiviral assay Antiviral activity against Herpes simplex virus 2 G infected in human HEL cells assessed as inhibition of virus-induced cytopathic effect, EC50=0.04μM. 28829913
HEL Antiviral assay Antiviral activity against wild type Herpes simplex virus 1 KOS infected in HEL cells assessed as inhibition of virus-induced cytopathic effect, EC50=0.04μM. 28757102
Vero Antiviral assay 36 hrs Antiviral activity against HSV 1 infected in African green monkey Vero cells assessed as reduction in plaque formation incubated for 36 hrs by crystal violet staining based assay, EC50=0.04μM. 32191456
OST TK- cells Cytotoxicity assay Cytotoxicity against human OST TK- cells expressing HSV1 TK in presence of 10 uM 1-[6-(triphenylmethoxy)hexyl]thymine, IC50=0.041μM. 17181158
HEL Antiviral assay 2 to 3 days Antiviral activity against Herpes simplex virus 1 KOS infected in HEL cells assessed as inhibition of plaque formation measured after 2 to 3 days post infection, EC50=0.045μM. 29550734
OST TK- cells Cytotoxicity assay Cytotoxicity against human OST TK- cells expressing HSV1 TK in presence of 2.5 uM 1-[6-[1,1-(diphenyl)-1-(4-pyridyl)methoxy]hexyl]thymine, IC50=0.05μM. 17181158
human Antiviral assay 7 days Antiviral activity against HSV2 G infected in human HEL cells assessed as reduction of virus-induced cytopathogenicity after 7 days, EC50=0.05μM. 21128666
HEL Antiviral assay 2 to 3 days Antiviral activity against Herpes simplex virus 2 G infected in HEL cells assessed as inhibition of virus-induced cytopathic effect measured after 2 to 3 days post-infection, EC50=0.051μM. 29670705
HEL Antiviral assay Antiviral activity against Herpes simplex virus 2 G infected in HEL cells assessed as reduction in virus-induced cytopathogenicity, EC50=0.055μM. 27750154
HEL Antiviral assay 2 to 3 days Antiviral activity against Herpes simplex virus 1 KOS infected in HEL cells assessed as inhibition of virus-induced cytopathic effect measured after 2 to 3 days post-infection, EC50=0.057μM. 29670705
HEL Function assay Compound was tested for anti-viral activity against HSV-1(G) in HEL cells, EC50=0.06μM. 14643328
Hel Function assay Effective concentration required to inhibit herpes simplex virus type 1 Kos strain induced cytopathicity in Hel cells, EC50=0.06μM. 15658858
HEL Antiviral assay Antiviral activity against HSV1 KOS in HEL cells, EC50=0.06μM. 16392791
HEL Antiviral assay Antiviral activity against HSV2 G in HEL cells, EC50=0.06μM. 16392791
HEL Antiviral assay 3 days Antiviral activity against Human herpesvirus 2 strain G infected human HEL cells assessed as reduction of virus-induced cytopathic effect after 3 days by colorimetric formazan-based MTS assay, EC50=0.07μM. ChEMBL
HEL Antiviral assay Antiviral activity against herpes simplex virus 1 KOS infected in HEL cells assessed as reduction in virus-induced cytopathogenicity by microscopic analysis, EC50=0.07μM. 30738653
HELF Antiviral assay 7 days Antiviral activity against Herpes simplex virus 1 KOS infected in human HELF cells assessed as reduction in virus-induced cytopathogenicity incubated for 7 days by microscopic analysis, EC50=0.07μM. 31586832
HEL Antiviral assay 7 days Antiviral activity against HSV1 KOS infected in human HEL cells assessed as reduction of virus-induced cytopathogenicity after 7 days, EC50=0.08μM. 21128666
HEL Antiviral assay 3 days Antiviral activity against Human herpesvirus 2 strain G infected HEL cells assessed as inhibition of virus-induced cytopathic effect after 3 days by MTT assay, EC50=0.08μM. ChEMBL
HEL Antiviral assay 3 days Antiviral activity against Human herpesvirus 1 strain KOS infected in HEL cells assessed as inhibition of virus-induced cytopathic effect after 3 days by MTT assay, EC50=0.08μM. ChEMBL
OST TK- cells Cytotoxicity assay Cytotoxicity against human OST TK- cells expressing HSV1 TK in presence of 5 uM 1-[6-[1,1-(diphenyl)-1-(4-pyridyl)methoxy]hexyl]thymine, IC50=0.081μM. 17181158
HEL Antiviral assay 3 days Antiviral activity against Human herpesvirus 1 strain KOS infected human HEL cells assessed as reduction of virus-induced cytopathic effect after 3 days by colorimetric formazan-based MTS assay, EC50=0.09μM. ChEMBL
HEL Antiviral assay Antiviral activity against Herpes simplex virus 1 KOS infected in HEL cells assessed as inhibition of virus plaque formation, EC50=0.09μM. 27639368
E6SM Antiviral assay Antiviral activity against HSV1 KOS-induced cytopathogenicity in E6SM cells, MIC=0.096μM. 17092728
E6SM Antiviral assay Antiviral activity against HSV2 G-induced cytopathogenicity in E6SM cells, MIC=0.096μM. 17092728
HEL Antiviral assay Antiviral activity against thymidine kinase deficient acyclovir-resistant HSV1 KOS infected in HEL cells assessed as inhibition of virus-induced cytopathic effect, EC50=0.1μM. 21232828
HEL Antiviral assay Antiviral activity against thymidine kinase-deficient ACV-resistant Herpes simplex virus 1 KOS infected in HEL cells assessed as inhibition of virus-induced cytopathic effect, EC50=0.1μM. 28757102
OST TK- cells Cytotoxicity assay Cytotoxicity against human OST TK- cells expressing HSV1 TK in presence of 10 uM 1-[6-[1,1-(diphenyl)-1-(4-pyridyl)methoxy]hexyl]thymine, IC50=0.11μM. 17181158
HFF Antiviral assay 10 days Antiviral activity against HCMV Towne infected in HFF cells incubated for 10 days by plaque reduction assay, IC50=0.14μM. 21812420
HFF Function assay Inhibition of HCMV AD169 replication in HFF cells by cytopathic effect assay, EC50=0.15μM. 17004726
MCA-TK Cytotoxicity assay Cytotoxicity against MCA-TK cells, IC50=0.15μM. 17960926
vero Antiviral assay Antiviral activity was determined in plaque reduction assay in vero cells against HSV-1 F strain, ID50=0.2μM. 3871860
Vero Antiviral assay In vitro antiviral activity was measured against HSV-1(F) in Vero cells, ID50=0.2μM. 3009811
Vero Antiviral assay Antiviral activity determined against herpes simplex type 1 (F strain) by plaque reduction in Vero cells, ID50=0.2μM. 3016263
HEL Antiviral assay Antiviral activity against HSV2 G in human HEL cells assessed as protection against virus-induced cytopathicity, EC50=0.2μM. 19281225
HEL Antiviral assay 3 days Antiviral activity against acyclovir-resistant TK-defecient Herpes simplex virus 1 KOS infected in human HEL cells assessed as inhibition of virus-induced cytopathic effect after 3 days by microscopic method, EC50=0.2μM. 30286952
fibroblast cells Function assay Inhibition of viral cytopathic effect in infected human foreskin fibroblast cell in monolayers of HSV-2 AD Vero cells by 50%, EC50=0.23μM. 11585457
HEL Antiviral assay Antiviral activity against Cytomegalovirus AD169 infected in HEL cells, EC50=0.25μM. 27128178
vero Antiviral assay In vitro antiviral and anticellular activity was evaluated against herpes simplex virus HSV-1 (F strain) in vero cells tissue culture, ID50=0.3μM. 2993615
HELF Antiviral assay 7 days Antiviral activity against Herpes simplex virus 2 G infected in human HELF cells assessed as reduction in virus-induced cytopathogenicity incubated for 7 days by microscopic analysis, EC50=0.3μM. 31586832
HS27 Antiviral assay 7 days Antiviral activity against human cytomegalovirus infected in human HS27 cells after 7 days by GFP-based fluorescent reduction assay, EC50=0.32μM. 20047911
HEL Antiviral assay Antiviral activity against Cytomegalovirus Davis infected in human HEL cells assessed as reduction in virus plaque formation, EC50=0.38μM. 23911856
HEL Antiviral assay Antiviral activity against Cytomegalovirus Davis 07/1 infected in HEL cells, EC50=0.4μM. 27128178
HEL Antiviral assay Antiviral activity against herpes simplex virus 2 G infected in HEL cells assessed as reduction in virus-induced cytopathogenicity by microscopic analysis, EC50=0.4μM. 30738653
HEL Antiviral assay Antiviral activity against HSV1 KOS in HEL cells, EC50=0.48μM. 17181162
HFF Function assay Inhibitory concentration of the drug against the cytopathic effect for AD169 strain of epstein barr virus-2 (HCMV) in human HFF cells, EC50=0.5μM. 8394933
HEL Function assay Inhibition of human CMV DNA synthesis in CMV-infected HEL cells, IC50=0.5μM. 7752205
HEL Antiviral assay Antiviral activity against thymidine kinase-deficient acyclovir-resistant Herpes simplex virus 1 KOS infected in HEL cells assessed as reduction of virus-induced cytopathogenicity, EC50=0.5μM. 26001344
HELF Antiviral assay Antiviral activity against thymidine kinase-deficient acyclovir-resistant Human herpesvirus 1 KOS strain infected in HELF cells assessed as inhibition of virus-induced cytopathogenicity, EC50=0.5μM. 30098481
Vero Antiviral assay 36 hrs Antiviral activity against HSV 2 infected in African green monkey Vero cells assessed as reduction in plaque formation incubated for 36 hrs by crystal violet staining based assay, EC50=0.58μM. 32191456
HEL Antiviral assay 5 days Antiviral activity against Varicella zoster virus OKA infected in human HEL cells assessed as inhibition of virus-induced cytopathicity after 5 days, EC50=0.59μM. 19339082
HEL Antiviral assay Antiviral activity against wild type HCMV Davis infected in HEL cells assessed as inhibition of virus-induced cytopathicity, EC50=0.66μM. 19226140
HEL Antiviral assay Antiviral activity against acyclovir-resistant HCMV AD169 clone 1 infected in HEL cells assessed as inhibition of virus-induced cytopathicity, EC50=0.69μM. 19226140
HEL Antiviral assay Antiviral activity against wild type HCMV AD169 infected in HEL cells assessed as inhibition of virus-induced cytopathicity, EC50=0.7μM. 19226140
Vero Antiviral assay 36 to 48 hrs Antiviral activity against Herpes simplex virus 1 F infected in african green monkey Vero cells assessed as plaque reduction after 36 to 48 hrs, EC50=0.7μM. 17438061
HFF Antiviral assay 8 to 10 days Antiviral activity against HCMV TB40 infected in human HFF cells assessed as reduction in plaque formation treated with compound following viral infection and incubated for 8 to 10 days by crystal violet staining based assay, EC50=0.72μM. 32191456
fibroblast cells Antiviral assay 1 hour Antiviral activity against human cytomegalovirus Ad169 (WT) plaque forming unit grown on Human foreskin fibroblast cells upon incubation for 1 hour at 37 degrees C with compound dissolved in DMSO, IC50=0.8μM. 16134946
fibroblast cells Antiviral assay 1 hour Antiviral activity against human cytomegalovirus Ad169 (V823A) plaque forming unit grown on Human foreskin fibroblast cells upon incubation for 1 hour at 37 degrees C with compound dissolved in DMSO, IC50=0.8μM. 16134946
E6SM Antiviral assay Antiviral activity against HSV1 TK- KOS ACV-induced cytopathogenicity in E6SM cells, MIC=0.8μM. 17092728
HEL Antiviral assay 4 days Antiviral activity against acyclovir-resistant TK-deficient Herpes simplex virus-1 KOS infected in human HEL cells assessed as inhibition of virus-induced cytopathic effect after 4 days by microscopic analysis, EC50=0.8μM. 21696963
HEL Antiviral assay Antiviral activity against acyclovir-resistant thymidine kinase deficient Herpes simplex virus 1 KOS infected in HEL cells assessed as inhibition of virus-induced cytopathicity, EC50=0.8μM. 29880251
HEL Antiviral assay Antiviral activity against thymidine kinase deficient and aciclovir resistant herpes simplex virus 1 KOS infected in HEL cells assessed as reduction in virus-induced cytopathogenicity by microscopic analysis, EC50=0.8μM. 30738653
HELF Antiviral assay 7 days Antiviral activity against Herpes simplex virus 1 TK-KOS-ACV infected in human HELF cells assessed as reduction in virus-induced cytopathogenicity incubated for 7 days by microscopic analysis, EC50=0.8μM. 31586832
HEL Antiviral assay Antiviral activity against Herpes simplex virus-2 G infected in human HEL cells assessed as reduction in virus-induced cytopathogenicity, EC50=0.9μM. 23911856
CRFK Antiviral assay Antiviral activity against Feline Herpes virus infected in cat CRFK cells assessed as inhibition of virus-induced cytopathic effect by MTS assay, EC50=0.9μM. 23911856
Vero Antiviral assay 3 days Antiviral activity against Herpes simplex virus 1 strain F VR-733 infected in African green monkey Vero cells after 3 days by MTT assay, EC50=0.9μM. 26460883
MRC5 Antiviral assay Antiviral activity against HCMV in MRC5 cells by plaque reduction assay, IC50=0.91μM. 17239594
MEF Antiviral assay 3 days Antiviral activity against Mouse Cytomegalovirus Smith (ATCC VR-1399) infected in mouse MEF cells assessed as reduction in plaque formation measured after 3 days by crystal violet staining based assay, EC50=0.96μM. 32191456
HFF Antiviral assay 5 days Antiviral activity against HCMV infected HFF cells after 5 days by dot blot assay, IC50=1μM. 17329103
HEL Antiviral assay Antiviral activity against ACV-resistant TK-deficient HSV-1 KOS infected in HEL cells assessed as inhibition of viral-induced cytopathogenicity, EC50=1μM. 23795238
HFF Antiviral assay Antiviral activity against Cytomegalovirus CMV T2211 infected in HFF cells by SEAP reporter gene assay, EC50=1.1μM. 17709468
HEL Antiviral assay 5 days Antiviral activity against Varicella zoster virus YS infected in human HEL cells assessed as inhibition of virus induced cytopathicity after 5 days, EC50=1.1μM. 19339082
CRFK Antiviral assay Antiviral activity against Feline herpesvirus infected in CRFK cells by viral CPE assay, EC50=1.15μM. 22858222
HEL Antiviral assay Antiviral activity against HPMPA-resistant HCMV AD169 clone 2 mutant infected in HEL cells assessed as inhibition of virus-induced cytopathicity, EC50=1.2μM. 19226140
fibroblast cells Antiviral assay Antiviral activity against Human cytomegalovirus Towne infected in human foreskin fibroblast cells by plaque reduction assay, EC50=1.2μM. 19397271
HFF Function assay Inhibitory concentration required against human cytomegalovirus (HCMV) wild type expressed in HFF cells was determined by plaque reduction assay, IC50=1.2μM. 15509176
HFF Function assay Inhibitory concentration required against human cytomegalovirus (HCMV) D10 strain (UL89 mutant gene) expressed in HFF cells was determined by plaque reduction assay, IC50=1.2μM. 15509176
HFF Function assay Inhibitory concentration required against human cytomegalovirus (HCMV) r56 strain (UL56 mutant gene) expressed in HFF cells was determined by plaque reduction assay, IC50=1.2μM. 15509176
FRCK Antiviral assay Antiviral activity against Feline herpesvirus in feline FRCK cells assessed as inhibition of virus-induced cytopathicity, EC50=1.2μM. 19281225
CRFK Antiviral assay 3 days Antiviral activity against Feline herpesvirus infected in CRFK cells assessed as inhibition of virus-induced cytopathicity after 3 days, EC50=1.2μM. 23047229
vero Antiviral assay In vitro antiviral and anticellular activity was evaluated against herpes simplex virus HSV-2 (G strain) in vero cells, ID50=1.3μM. 2993615
fibroblast cells Antiviral assay Antiviral activity against human cytomegalovirus (Davis strain) grown on human foreskin fibroblast cells determined by reduction in plaque formation upon incubation at 37 degrees C with the compound dissolved in DMSO, IC50=1.3μM. 16134946
HFF Antiviral assay Antiviral activity against Human CMV T2211 in HFF cells by SEAP assay, IC50=1.3μM. 17043128
HEL Antiviral assay 7days Antiviral activity against human cytomegalovirus Davis in HEL cells assessed as reduction of plaque formation after 7days, EC50=1.3μM. 17539622
HFF Antiviral assay Antiviral activity against HCMV Davis in HFF cells by plaque reduction assay, IC50=1.3μM. 17434304
HEL Antiviral assay 3 days Antiviral activity against HCMV Davis infected in HEL cells assessed as reduction of virus-induced cytopathogenicity after 3 days, EC50=1.3μM. 17961851
HFF Antiviral assay Antiviral activity against Human cytomegalovirus infected in HFF cells by plaque reduction assay, IC50=1.3μM. 20167488
HFF Function assay Inhibition of HCMV DNA polymerase infected in HFF cells, IC50=1.3μM. 20403696
MRC5 Function assay Inhibitory concentration against Davis strain of HCMV in MRC5 cells, IC50=1.4μM. 11454465
HFF Antiviral assay Antiviral activity against Human CMV T2241 in HFF cells by SEAP assay, IC50=1.4μM. 17043128
HFF Antiviral assay Antiviral activity against Human CMV T2233 in HFF cells by SEAP assay, IC50=1.4μM. 17043128
Vero Antiviral assay 3 days Antiviral activity against Herpes simplex virus 2 strain MS VR-540 infected in African green monkey Vero cells after 3 days by MTT assay, EC50=1.4μM. 26460883
MRC5 Function assay Inhibitory concentration against Towne strain of HCMV in MRC5 cells, IC50=1.5μM. 11454465
CCL81 Antiviral assay Antiviral activity against HSV1 in CCL81 cells, EC50=1.5μM. 17188402
CCL81 Antiviral assay Antiviral activity against HSV2 in CCL81 cells, EC50=1.5μM. 17188402
MRC5 Function assay Inhibitory concentration against 2599R strain of HCMV in MRC5 cells, IC50=1.6μM. 11454465
Vero Antiviral assay In vitro antiviral activity was measured against HSV-2(G) in Vero cells, ID50=1.6μM. 3009811
HFF Function assay In vitro reduction in yield of Towne strain of HCMV in HFF (human fibroblast) cells, IC90=1.6μM. 9057865
HFF Function assay Inhibitory concentration against human cytomegalovirus replication in HFF cells was determined by yield reduction assay, IC90=1.6μM. 9438017
HFF Function assay Compound was tested for antiviral activity against Towne strain of HCMV in a yield reduction assay using HFF cells, IC90=1.6μM. 10882374
HFF Function assay Compound was tested for antiviral activity against human cytomegalovirus (HCMV) by yield reduction assay using HFF cells, IC90=1.6μM. 10882375
HFF Antiviral assay Antiviral activity against towne strain HCMV was determined by yield reduction assay in duplicate wells using HFF cells, IC90=1.6μM. 10882370
HFF Antiviral assay Antiviral activity against HCMV was determined by yield reduction assay using HFF cells, IC90=1.6μM. 10882372
MRC5 Antiviral assay 7 days Antiviral activity against human cytomegalovirus infected in human MRC5 cells after 7 days by GFP-based fluorescent reduction assay, EC50=1.65μM. 20047911
HEL Antiviral assay 4 days Antiviral activity against Cytomegalovirus AD-169 infected in HEL cells assessed as inhibition of virus plaque formation after 4 days, EC50=1.65μM. 22459876
HEL Antiviral assay 4 days Antiviral activity against Human cytomegalovirus Davis infected in HEL cells assessed as inhibition of virus plaque formation after 4 days, EC50=1.65μM. 22459876
HFF Function assay Inhibition of HCMV towne replication in HFF cells by plaque reduction assay, EC50=1.8μM. 17004726
HFF Antiviral assay Antiviral activity against Human CMV AD169 in HFF cells by PRA, IC50=1.9μM. 17043128
HEL Antiviral assay 7 days Antiviral activity against HCMV AD169 infected in human HEL cells assessed inhibition of virus-induced cytopathicity after 7 days postinfection, EC50=2μM. 19226140
HFF Function assay Inhibition of HCMV Towne replication in HFF cells by plaque reduction assay, EC50=2.1μM. 15615545
HFF Antiviral assay 8 days Antiviral activity against HCMV infected in human HFF cells assessed as reduction in cytopathic effect after 8 days by plaque reduction assay, IC50=2.1μM. 22607883
HEL Antiviral assay Antiviral activity against Human cytomegalovirus Davis infected in HEL cells assessed as inhibition of virus plaque formation, EC50=2.1μM. 27639368
HFF Antiviral assay 8 to 10 days Antiviral activity against HCMV Towne infected in human HFF cells assessed as reduction in plaque formation treated with compound following viral infection and incubated for 8 to 10 days by crystal violet staining based assay, EC50=2.1μM. 32191456
Vero Antiviral assay 3 days Antiviral activity against Human cytomegalovirus Davis VR-807 infected in African green monkey Vero cells after 3 days by MTT assay, EC50=2.14μM. 26460883
HFF Function assay Inhibition of HCMV AD169 replication in HFF cells by CPE inhibition assay, EC50=2.2μM. 15615545
HEL Antiviral assay Antiviral activity against Human cytomegalovirus Davis infected in HEL cells assessed as inhibition of virus-induced cytopathicity, EC50=2.29μM. 29880251
HEL Antiviral assay 5 days Antiviral activity against TK+ VZV YS in HEL cells assessed as reduction of virus plaque formation after 5 days, EC50=2.3μM. 17622128
HELF Antiviral assay 7 days Antiviral activity against Cytomegalovirus Davis infected in human HELF cells assessed as reduction in virus-induced cytopathogenicity incubated for 7 days by Giemsa staining based microscopic analysis, EC50=2.3μM. 31586832
HEL299 Antiviral assay 7 days Antiviral activity against human cytomegalovirus infected in HEL299 cells after 7 days by GFP-based fluorescent reduction assay, EC50=2.39μM. 20047911
HFF Function assay Inhibitory concentration required against human cytomegalovirus (HCMV) C4 strain (UL56 + UL89 mutant gene) expressed in HFF cells was determined by plaque reduction assay, IC50=2.5μM. 15509176
HFF Antiviral assay Antiviral activity against Human cytomegalovirus Towne infected in HFF cells by plaque reduction assay, EC50=2.5μM. 18082410
HEL Antiviral assay 7 days Antiviral activity against human cytomegalovirus Davis infected in human HEL cells assessed as inhibition of virus-induced cytopathicity after 7 days post infection, EC50=2.5μM. 19339082
HEL Antiviral assay 5 days Antiviral activity against thymidine kinase deficient Varicella zoster virus 07/1 infected in human HEL cells assessed as inhibition of virus-induced cytopathicity after 5 days, EC50=2.5μM. 19339082
HFF Function assay Inhibitory concentration against human cytomegalovirus replication in HFF cells was determined by cytopathic effect inhibition assay, IC50=2.6μM. 9438017
HEL Antiviral assay Antiviral activity against foscarnet-resistant HCMV AD169 clone C infected in HEL cells assessed as inhibition of virus-induced cytopathicity, EC50=2.6μM. 19226140
HFF Antiviral assay Antiviral activity against Cytomegalovirus CMV T2417 with pol A809V mutant infected in HFF cells by SEAP reporter gene assay, EC50=2.6μM. 17709468
HEL Antiviral assay 7 days Antiviral activity against CMV Davis in human HEL cells after 7 days, EC50=2.6μM. 17672445
CRFK Antiviral assay Antiviral activity against Feline herpesvirus infected in cat CRFK cells assessed as protection from virus-induced cytopathogenicity by MTT assay, EC50=2.6μM. 20034711
HFF Antiviral assay Antiviral activity against Cytomegalovirus CMV T2542 with pol T813S mutant infected in HFF cells by SEAP reporter gene assay, EC50=2.7μM. 17709468
HEL Antiviral assay Antiviral activity against HCMV Davis in HEL cells assessed as reduction of virus plaque formation, EC50=2.75μM. 17518459
HEL Antiviral assay Antiviral activity against Human cytomegalovirus Davis infected in HEL cells assessed as inhibition of virus-induced cytopathic effect, EC50=2.96μM. 28757102
BSC-1 Antiviral assay Antiviral activity of the compound was evaluated against the Herpes simplex virus type-1 in BSC-1 cells, IC50=3μM. 2913300
MRC5 Function assay Inhibitory concentration against AD169 strain of HCMV in MRC5 cells, IC50=3μM. 11454465
HFF Antiviral assay Antiviral activity against HCMV Towne in HFF cells by plaque reduction assay, IC50=3μM. 17161946
hhTERT-BJ1 Antiviral assay 2 days Antiviral activity against Human cytomegalovirus Towne infected in human hhTERT-BJ1 cells assessed as inhibition of virus mediated DNA synthesis after 2 days, EC50=3μM. 18458124
HEL Antiviral assay 7 days Antiviral activity against acyclovir-resistant thymidine kinase deficient HSV1 KOS infected in human HEL cells assessed as reduction of virus-induced cytopathogenicity after 7 days, EC50=3μM. 21128666
HFF Antiviral assay 10 days Antiviral activity against Human cytomegalovirus Towne infected in HFF cells assessed as reduction in plaque formation after 10 days, IC50=3μM. 21641218
HEL Antiviral assay Antiviral activity against Cytomegalovirus AD-169 infected in human HEL cells assessed as reduction in virus plaque formation, EC50=3.1μM. 23911856
HEL Antiviral assay Antiviral activity against Human cytomegalovirus AD169 infected in HEL cells assessed as inhibition of virus plaque formation, EC50=3.1μM. 27639368
HELF Antiviral assay 7 days Antiviral activity against Cytomegalovirus AD169 infected in human HELF cells assessed as reduction in virus-induced cytopathogenicity incubated for 7 days by Giemsa staining based microscopic analysis, EC50=3.15μM. 31586832
HFF Antiviral assay Antiviral activity against ganciclovir-sensitive HCMV1 isolate replication in HFF cells by plaque reduction assay, IC50=3.22μM. 19010684
HFF Function assay Compound was tested for antiviral activity against wild-type AD169 strain of HCMV in yield reduction assay using HFF cells, IC90=3.3μM. 10882374
HEL Antiviral assay 6 to 7 days Antiviral activity against Human cytomegalovirus Davis infected in HEL cells assessed as inhibition of virus-induced cytopathic effect measured after 6 to 7 days post-infection, EC50=3.31μM. 29670705
MEF Function assay Inhibitory concentration against murine cytomegalovirus replication in MEF cells was determined by plaque reduction assay, IC50=3.4μM. 9438017
BSC-1 Antiviral assay Antiviral activity against HSV-1 (herpes simplex virus), determined by ELISA in quadruplicate wells using BSC-1 cells, IC50=3.5μM. 10882370
BSC-1 Function assay ELISA assay was performed using BSC-1 cells to determine activity against HSV-1, IC50=3.5μM. 10882372
BSC-1 Antiviral assay In vitro antiviral activity against HSV-1 virus in BSC-1 (monkey kidney) cells, IC50=3.5μM. 9057865
HEL Antiviral assay Antiviral activity against PMEDAP-resistant HCMV AD169 clone 4 infected in HEL cells assessed as inhibition of virus-induced cytopathicity, EC50=3.5μM. 19226140
HFF Antiviral assay Antiviral activity against Cytomegalovirus CMV T2420 with pol G841A mutant infected in HFF cells by SEAP reporter gene assay, EC50=3.5μM. 17709468
BSC-1 Function assay Inhibitory concentration required against herpes simplex virus type-1 (HSV-1) expressed in BSC-1 cells was determined by ELISA assay, IC50=3.5μM. 15509174
BSC-1 Function assay Inhibitory concentration required against herpes simplex virus type-1 (HSV-1) expressed in BSC-1 cells was determined by ELISA assay, IC50=3.5μM. 15509175
BSC-1 Function assay Inhibitory concentration required against herpes simplex virus type-1 (HSV-1) expressed in BSC-1 cells was determined by ELISA assay, IC50=3.5μM. 15509176
HFF Function assay Compound was tested for antiviral activity against 2916 AD169 strain of HCMV in yield reduction assay using HFF cells, IC90=3.6μM. 10882374
HEL Antiviral assay 7 days Antiviral activity against HCMV Davis infected in human HEL cells assessed inhibition of virus-induced cytopathicity after 7 days postinfection, EC50=3.6μM. 19226140
HFF Antiviral assay Antiviral activity against Cytomegalovirus CMV T2258 with UL97 C592G mutant infected in HFF cells by SEAP reporter gene assay, EC50=3.9μM. 17709468
HEL Antiviral assay 2 to 3 days Antiviral activity against thymidine kinase-deficient ACV-resistant Herpes simplex virus 1 KOS infected in HEL cells assessed as inhibition of plaque formation measured after 2 to 3 days post infection, EC50=3.91μM. 29550734
MRC-5 Antiherpesvirus assay In vitro antiherpesvirus activity against MRC-5 cells infected with HCMV (AD-169 strain), IC50=4μM. 1323678
HEL Antiviral assay Antiviral activity against cidofovir HCMV AD169 clone 5 infected in human HEL cells assessed as inhibition of virus-induced cytopathicity, EC50=4μM. 19226140
HEL Antiviral assay Antiviral activity against thymidine kinase deficient and acyclovir-resistant HSV1 KOS infected in HEL cells assessed as inhibition of virus-induced cytopathic effect, EC50=4μM. 23099097
HEL Antiviral assay Antiviral activity against TK-negative Herpes simplex virus 1 KOS infected in human HEL cells assessed as reduction of virus-induced cytopathogenicity, EC50=4μM. 23811093
HEL Antiviral assay Antiviral activity against thymidine kinase-deficient acyclovir-resistant Herpes simplex virus 1 KOS infected in HEL cells assessed as reduction in virus-induced cytopathogenicity, EC50=4μM. 27750154
HEL Antiviral assay Antiviral activity against Human cytomegalovirus Davis infected in HEL cells assessed as reduction in viral cytopathic effect, EC50=4.05μM. 29407990
HFF Function assay Compound was tested for antiviral activity against C-4 Towne strain of HCMV in yield reduction assay using HFF cells, IC50=4.1μM. 10882374
HFF Antiviral assay Antiviral activity against Human CMV T2291 in HFF cells by PRA, IC50=4.1μM. 17043128
CRFK Antiviral assay 4 days Antiviral activity against Feline herpesvirus infected in cat CRFK cells assessed as inhibition of virus-induced cytopathic effect after 4 days by colorimetric formazan-based MTS assay, EC50=4.1μM. 22459876
CRFK Antiviral assay Antiviral activity against feline herpesvirus infected in cat CRFK cells assessed as reduction of virus-induced cytopathogenicity by MTS assay, EC50=4.1μM. 23811093
HEL Antiviral assay Antiviral activity against ganciclovir-resistant HCMV AD169 clone 4 infected in HEL cells assessed as inhibition of virus-induced cytopathicity, EC50=4.3μM. 19226140
HEL Antiviral assay Antiviral activity against ACV-resistant thymidine kinase deficient Herpes simplex virus 1 KOS infected in human HEL cells assessed as inhibition of virus-induced cytopathic effect, EC50=4.3μM. 28829913
HFF Function assay Compound was tested for antiviral activity against wild-type Towne strain of HCMV in yield reduction assay using HFF cells, IC50=4.5μM. 10882374
HEL Antiviral assay 7 days Antiviral activity against Human cytomegalovirus Davis expressed in HEL cells assessed as reduction in plaque formation incubated for 7 days by Giemsa stain based microscopy, EC50=4.72μM. 26291038
HEL Antiviral assay 7 days Antiviral activity against human cytomegalovirus AD169 in HEL cells assessed as reduction of plaque formation after 7 days, EC50=4.8μM. 17539622
HEL Antiviral assay 3 days Antiviral activity against HCMV AD169 infected in HEL cells assessed as reduction of virus-induced cytopathogenicity after 3 days, EC50=4.8μM. 17961851
HEL Antiviral assay 6 to 7 days Antiviral activity against Human cytomegalovirus Davis infected in HEL cells assessed as inhibition of plaque formation measured after 6 to 7 days post infection, EC50=4.83μM. 29550734
MRC5 Antiviral assay Antiviral activity against Human CMV Towne in MRC5 cells by PRA, IC50=4.9μM. 17043128
MRC5 Antiviral assay In vitro antiviral activity was measured against HCMV(AD-169) in MRC5 cells, ID50=5μM. 3009811
H-1 Function assay Effective concentration required to inhibit Epstein-barr virus replication in H-1 cells in DNA hybridization assay, EC50=5μM. 15634003
H1 Function assay Inhibition of EBV replication in H1 cells by DNA hybridization assay, EC50=5μM. 17004726
H1 Function assay Inhibition of EBV replication in H1 cells by DNA hybridization assay, EC50=5μM. 15615545
CEM Cytotoxicity assay Cytotoxicity against CEM cells, CC50=5μM. 15615545
HFF Antiviral assay 5 to 7 days Antiviral activity against HCMV infected HFF cells after 5 to 7 days by viral yield assay, EC99.9=5μM. 17329103
HEL Antiviral assay 7 days Antiviral activity against HCMV AD169 in HEL cells assessed as reduction of virus plaque formation after 7 days, EC50=5.1μM. 17622128
HEL Antiviral assay 7 days Antiviral activity against HCMV AD169/Davis infected in human HEL cells assessed as reduction of virus-induced cytopathogenicity after 7 days, EC50=5.2μM. 21128666
AKATA Antiviral assay 48 hrs Antiviral activity against EBV infected in human AKATA cells assessed as reduction in IgG-induced lytic virus replication measured at 48 hrs post-lytic induction by qRT-PCR analysis, EC50=5.2μM. 32191456
MRC5 Antiviral assay Antiviral activity against Human CMV T2296 in MRC5 cells by PRA, IC50=5.4μM. 17043128
fibroblast cells Antiviral assay Antiviral activity against Murine cytomegalovirus infected in mouse embryonic fibroblast cells by plaque reduction assay, EC50=5.5μM. 19397271
HFF Antiviral assay Antiviral activity against Human CMV T2293 in HFF cells by SEAP assay, IC50=5.6μM. 17043128
HEL Antiviral assay Antiviral activity against Human cytomegalovirus AD169 infected in HEL cells assessed as reduction in viral cytopathic effect, EC50=5.63μM. 29407990
NIH 3T3 Antiviral assay 4 to 5 days Antiviral activity against Murine cytomegalovirus strain Smith infected in mouse NIH 3T3 cells after 4 to 5 days by plaque reduction assay, EC50=5.7μM. 18458124
RG2TK+ Cytotoxicity assay 72 hrs Cytotoxicity against HSV1-tk gene overexpressing RG2TK+ cells after 72 hrs by MTT assay, CC50=5.86μM. 18800764
MRC-5 Function assay Concentration required to reduce viral plaque formation by Human Cytomegalovirus (HCMV) (strain AD-169) in MRC-5 cells, IC50=5.9μM. 10966740
HEL Antiviral assay Antiviral activity against HCMV Davis infected in HEL cells by viral CPE assay, EC50=5.9μM. 22858222
HEL Antiviral assay Antiviral activity against Cytomegalovirus Davis infected in human HEL cells assessed as reduction of plaque formation, EC50=5.98μM. 23811093
HCMV AD 169 Antiviral assay In vitro antiviral and anticellular activity was evaluated against human cytomegalovirus (HCMV AD 169) in (HCMV AD 169) cells, ID50=6μM. 2993615
HEL Antiviral assay Antiviral activity against Human cytomegalovirus AD169 infected in HEL cells assessed as protection against virus-induced cytopathicity, EC50=6μM. 21565516
HEL Antiviral assay 3 days Antiviral activity against acyclovir-resistant TK-deficient Hepres simplex virus 1 KOS infected in HEL cells assessed as inhibition of virus-induced cytopathic effect after 3 days by MTT assay, EC50=6μM. ChEMBL
HFF Antiviral assay Antiviral activity against Cytomegalovirus CMV T2784 with pol A809V UL97 C592G double mutant infected in HFF cells by SEAP reporter gene assay, EC50=6.1μM. 17709468
HEL Antiviral assay 7 days Antiviral activity against Human cytomegalovirus AD169 expressed in HEL cells assessed as reduction in plaque formation incubated for 7 days by Giemsa stain based microscopy, EC50=6.12μM. 26291038
MRC-5 Function assay Inhibitory concentration for anti-HCMV activity against AD-169 strain in MRC-5 cells, IC50=6.3μM. 9836613
HEL Antiviral assay Antiviral activity against HCMV AD169 in HEL cells assessed as reduction of virus plaque formation, EC50=6.3μM. 17518459
HEL Antiviral assay Antiviral activity against Cytomegalovirus AD-169 infected in HEL cells assessed as reduction of virus-induced cytopathogenicity, EC50=6.3μM. 26001344
HEL Antiviral assay 6 to 7 days Antiviral activity against Human cytomegalovirus AD169 infected in HEL cells assessed as inhibition of plaque formation measured after 6 to 7 days post infection, EC50=6.46μM. 29550734
HEL Antiviral assay 7 days Antiviral activity against CMV AD169 in human HEL cells after 7 days, EC50=6.5μM. 17672445
HEL Antiviral assay Antiviral activity against Cytomegalovirus Davis infected in HEL cells assessed as reduction in virus plaque formation, EC50=6.5μM. 27750154
HELF Antiviral assay Antiviral activity against Human cytomegalovirus Davis strain infected in HELF cells assessed as inhibition of virus-induced cytopathogenicity, EC50=6.52μM. 30098481
HEL Antiviral assay Antiviral activity against Human cytomegalovirus Davis infected in HEL cells assessed as protection against virus-induced cytopathicity, EC50=6.54μM. 21565516
HFF Antiviral assay Antiviral activity against Cytomegalovirus CMV T2798 with pol T813S UL97 C592G double mutant infected in HFF cells by SEAP reporter gene assay, EC50=6.6μM. 17709468
HEL Antiviral assay Antiviral activity against Human cytomegalovirus AD169 infected in HEL cells assessed as inhibition of virus-induced cytopathic effect, EC50=6.79μM. 28757102
HEL Function assay Inhibition against DNA of uninfected HEL cells by incorporation of [3H]TdR, IC50=7μM. 7752205
HFF Antiviral assay Antiviral activity against Human CMV T2291 in HFF cells by SEAP assay, IC50=7.1μM. 17043128
HEL Antiviral assay Antiviral activity against Human cytomegalovirus Davis infected in HEL cells assessed as reduction in virus-induced cytopathicity, EC50=7.1μM. 22578783
HEL Antiviral assay Antiviral activity against Cytomegalovirus Davis infected in HEL cells assessed as reduction of virus-induced cytopathogenicity, EC50=7.1μM. 26001344
CRFK Antiviral assay 3 days Antiviral activity against Felid herpesvirus 1 infected cat CRFK cells assessed as reduction of virus-induced cytopathic effect after 3 days by colorimetric formazan-based MTS assay, EC50=7.3μM. ChEMBL
HEL Antiviral assay Antiviral activity against human cytomegalovirus Davis infected in human HEL cells assessed as inhibition of virus-induced cytopathic effect, EC50=7.3μM. 28829913
HFF Antiviral assay In vitro antiviral activity against HCMV (Towne strain) in HFF (human fibroblast) cells, IC50=7.4μM. 9057865
HFF Function assay Inhibitory concentration against human cytomegalovirus replication in HFF cells was determined by plaque reduction assay, IC50=7.4μM. 9438017
HFF Function assay Compound was tested for antiviral activity against Towne strain of HCMV in a plaque reduction assay using HFF cells, IC50=7.4μM. 10882374
HFF Function assay Compound was tested for antiviral activity against human cytomegalovirus (HCMV) by plaque reduction assay using HFF cells, IC50=7.4μM. 10882375
HFF Antiviral assay Antiviral activity against towne strain HCMV was determined by plaque reduction assay in duplicate wells using HFF cells, IC50=7.4μM. 10882370
HFF Antiviral assay Antiviral activity against HCMV was determined by plaque reduction assay using HFF cells, IC50=7.4μM. 10882372
HFF Function assay Inhibitory concentration required against human cytomegalovirus (HCMV) expressed in HFF cells was determined by plaque reduction assay, IC50=7.4μM. 15509174
HFF Function assay Inhibitory concentration required against human cytomegalovirus (HCMV) expressed in HFF cells was determined by plaque reduction assay, IC50=7.4μM. 15509175
HFF Function assay Inhibitory concentration required against human cytomegalovirus (HCMV) expressed in HFF cells was determined by plaque reduction assay, IC50=7.4μM. 15509176
HEL Antiviral assay Antiviral activity against human cytomegalovirus AD-169 infected in human HEL cells assessed as inhibition of virus-induced cytopathic effect, EC50=7.4μM. 28829913
HEL Antiviral assay 6 to 7 days Antiviral activity against Human cytomegalovirus AD169 infected in HEL cells assessed as inhibition of virus-induced cytopathic effect measured after 6 to 7 days post-infection, EC50=7.41μM. 29670705
HEL Antiviral assay Antiviral activity against Human cytomegalovirus AD169 infected in HEL cells assessed as reduction in virus-induced cytopathicity, EC50=7.5μM. 22578783
HFF Antiviral assay Antiviral activity was determined by the HCMV plaque assay using HFF cells, IC50=7.7μM. 9057866
HEL Antiviral assay Antiviral activity against human cytomegalovirus Davis infected in HEL cells assessed as reduction in viral plaque formation, EC50=7.8μM. 26443550
HEL Antiviral assay Antiviral activity against Cytomegalovirus AD-169 infected in human HEL cells assessed as reduction of plaque formation, EC50=7.87μM. 23811093
CRFK Antiviral assay Antiviral activity against Feline herpesvirus infected in CRFK cells assessed as protection against virus-induced cytopathicity, EC50=8.1μM. 21565516
HEL Antiviral assay Antiviral activity against human Cytomegalovirus AD169 infected in HEL cells assessed as inhibition of virus-induced cytopathicity after, EC50=8.2μM. 23911854
HFF Function assay In vitro inhibition of human cytomegalovirus in HFF cells by plaque reduction assay, IC50=8.4μM. ChEMBL
HFF Antiviral assay Antiviral activity against Cytomegalovirus CMV 2817 with pol G841A UL97 C592G double mutant infected in HFF cells by SEAP reporter gene assay, EC50=8.4μM. 17709468
HEL Antiviral assay 7 days Antiviral activity against HCMV Davis in HEL cells assessed as reduction of virus plaque formation after 7 days, EC50=8.7μM. 17622128
HEL Antiviral assay Antiviral activity against Human cytomegalovirus AD169 infected in HEL cells assessed as inhibition of virus-induced cytopathic effect, EC50=8.7μM. 28682067
HEL Antiviral assay Antiviral activity against thymidine kinase deficient and acyclovir resistant HSV1 KOS in human HEL cells assessed as protection against virus-induced cytopathicity, EC50=9μM. 19281225
HEL Antiviral assay Antiviral activity against HCMV AD169 infected in HEL cells by viral CPE assay, EC50=9.1μM. 22858222
MRC-5 Function assay Inhibitory activity of compound against human cytomegalovirus (HCMV) in MRC-5 cells, EC50=9.4μM. 12723951
Vero Antiviral assay In vitro antiviral activity was measured against HSV-1 (F delta 305) in Vero cells, ID50=10μM. 3009811
HFF Antiviral assay Antiviral activity against Human CMV T2293 in HFF cells by PRA, IC50=10μM. 17043128
Vero Antiviral assay 3 days Antiviral activity against Varicella-zoster virus Ellen VR-1367 infected in African green monkey Vero cells after 3 days by MTT assay, EC50=11.1μM. 26460883
HEL Antiviral assay Antiviral activity against Human cytomegalovirus AD169 infected in HEL cells assessed as inhibition of virus-induced cytopathicity, EC50=11.43μM. 29880251
HELF Antiviral assay Antiviral activity against Human cytomegalovirus AD169 strain infected in HELF cells assessed as inhibition of virus-induced cytopathogenicity, EC50=11.75μM. 30098481
HFF Antiviral assay Antiviral activity against Human CMV T2311 in HFF cells by PRA, IC50=12.4μM. 17043128
HEL Antiviral assay 7 days Antiviral activity against human cytomegalovirus AD169 infected in human HEL cells assessed as inhibition of virus-induced cytopathicity after 7 days post infection, EC50=12.6μM. 19339082
HEL Antiviral assay Antiviral activity against human cytomegalovirus AD169 infected in HEL cells assessed as reduction in viral plaque formation, EC50=13.9μM. 26443550
Vero Antiviral assay 36 to 48 hrs Antiviral activity against Herpes B virus 24105 infected in african green monkey Vero cells assessed as plaque reduction after 36 to 48 hrs, EC50=14.5μM. 17438061
HEL Antiviral assay Antiviral activity against thymidine kinase negative and ACV resistant HSV1 KOS infected in HEL cells by viral CPE assay, EC50=14.7μM. 22858222
HEL Antiviral assay Antiviral activity against Cytomegalovirus AD169 infected in HEL cells assessed as reduction in virus plaque formation, EC50=14.9μM. 27750154
HEL Antiviral assay Antiviral activity against acyclovir-resistant Human herpesvirus 1 KOS infected in HEL cells assessed as protection against virus-induced cytopathicity, EC50=15.7μM. 21565516
HFF Antiviral assay Antiviral activity against ganciclovir-resistant HCMV3 isolate replication in HFF cells by plaque reduction assay, IC50=16.4μM. 19010684
HEL Antiviral assay Antiviral activity against HCMV Davis strain infected in HEL cells assessed as inhibition of virus-induced cytopathic effect, EC50=16.5μM. 28682067
Vero Antiviral assay 36 to 48 hrs Antiviral activity against Herpes B virus E90-136 infected in african green monkey Vero cells assessed as plaque reduction after 36 to 48 hrs, EC50=19.2μM. 17438061
Vero Antiviral assay Antiviral activity against Macacine herpesvirus 1 isolate E2490 infected in african green monkey Vero cells by plaque reduction assay, EC50=19.3μM. 19858259
Vero Antiviral assay Antiviral activity against Macacine herpesvirus 1 isolate MR9 infected in african green monkey Vero cells by plaque reduction assay, EC50=19.3μM. 19858259
Vero Antiviral assay Antiviral activity against Macacine herpesvirus 1 isolate MR4 infected in african green monkey Vero cells by plaque reduction assay, EC50=19.3μM. 19858259
Vero Antiviral assay Antiviral activity against Macacine herpesvirus 1 isolate MR5 infected in african green monkey Vero cells by plaque reduction assay, EC50=19.3μM. 19858259
Vero Antiviral assay Antiviral activity against Macacine herpesvirus 1 isolate MR6 infected in african green monkey Vero cells by plaque reduction assay, EC50=19.3μM. 19858259
Vero Antiviral assay Antiviral activity against Macacine herpesvirus 1 isolate MR2 infected in african green monkey Vero cells by plaque reduction assay, EC50=19.3μM. 19858259
Vero Antiviral assay Antiviral activity against Macacine herpesvirus 1 isolate A1 infected in african green monkey Vero cells by plaque reduction assay, EC50=19.3μM. 19858259
Vero Antiviral assay Antiviral activity against Macacine herpesvirus 1 isolate A2 infected in african green monkey Vero cells by plaque reduction assay, EC50=19.3μM. 19858259
Vero Antiviral assay Antiviral activity against Macacine herpesvirus 1 isolate A3 infected in african green monkey Vero cells by plaque reduction assay, EC50=19.3μM. 19858259
Vero Antiviral assay Antiviral activity against Macacine herpesvirus 1 isolate MR11 infected in african green monkey Vero cells by plaque reduction assay, EC50=19.3μM. 19858259
Vero Antiviral assay Antiviral activity against Macacine herpesvirus 1 isolate A5 infected in african green monkey Vero cells by plaque reduction assay, EC50=19.3μM. 19858259
Vero Antiviral assay Antiviral activity against Macacine herpesvirus 1 isolate MC1 infected in african green monkey Vero cells by plaque reduction assay, EC50=19.3μM. 19858259
Vero Antiviral assay Antiviral activity against Macacine herpesvirus 1 isolate MP1 infected in african green monkey Vero cells by plaque reduction assay, EC50=19.3μM. 19858259
Vero Antiviral assay Antiviral activity against HSV2 infected in african green monkey Vero cells by plaque reduction assay, EC50=19.3μM. 19858259
Vero Antiviral assay Antiviral activity against Macacine herpesvirus 1 isolate MR3 infected in african green monkey Vero cells by plaque reduction assay, EC50=19.3μM. 19858259
Vero Antiviral assay Antiviral activity against Macacine herpesvirus 1 isolate MR8 infected in african green monkey Vero cells by plaque reduction assay, EC50=19.3μM. 19858259
Vero Antiviral assay Antiviral activity against Macacine herpesvirus 1 isolate MR10 infected in african green monkey Vero cells by plaque reduction assay, EC50=19.3μM. 19858259
Vero Antiviral assay Antiviral activity against Macacine herpesvirus 1 isolate MR1 infected in african green monkey Vero cells by plaque reduction assay, EC50=19.3μM. 19858259
Vero Antiviral assay Antiviral activity against Macacine herpesvirus 1 isolate A4 infected in african green monkey Vero cells by plaque reduction assay, EC50=19.3μM. 19858259
Vero Antiviral assay Antiviral activity against Macacine herpesvirus 1 isolate MR7 infected in african green monkey Vero cells by plaque reduction assay, EC50=19.3μM. 19858259
Vero Antiviral assay Antiviral activity against Macacine herpesvirus 1 isolate MC2 infected in african green monkey Vero cells by plaque reduction assay, EC50=19.3μM. 19858259
Vero Antiviral assay Antiviral activity against Macacine herpesvirus 1 isolate MJ1 infected in african green monkey Vero cells by plaque reduction assay, EC50=19.3μM. 19858259
Vero Antiviral assay Antiviral activity against Macacine herpesvirus 1 isolate MJ2 infected in african green monkey Vero cells by plaque reduction assay, EC50=19.3μM. 19858259
Vero Antiviral assay Antiviral activity against Macacine herpesvirus 1 isolate MJ3 infected in african green monkey Vero cells by plaque reduction assay, EC50=19.3μM. 19858259
Vero Antiviral assay Antiviral activity against Macacine herpesvirus 1 isolate A6 infected in african green monkey Vero cells by plaque reduction assay, EC50=19.3μM. 19858259
Vero Antiviral assay Antiviral activity against HSV1 infected in african green monkey Vero cells by plaque reduction assay, EC50=19.3μM. 19858259
Vero Antiviral assay 36 to 48 hrs Antiviral activity against Herpes B virus 32425 infected in african green monkey Vero cells assessed as plaque reduction after 36 to 48 hrs, EC50=23.5μM. 17438061
HFF Antiviral assay 8 to 10 days Antiviral activity against GCV-resistant HCMV Towne infected in human HFF cells assessed as reduction in plaque formation treated with compound following viral infection and incubated for 8 to 10 days by crystal violet staining based assay, EC50=29.7μM. 32191456
bone marrow cells Cytotoxicity assay Cytotoxicity against human bone marrow cells assessed as inhibition of colony forming unit of granulocyte/macrophage, IC50=30μM. 16814545
bone marrow cells Cytotoxicity assay 15 days Cytotoxicity against human bone marrow cells assessed as inhibition of CFU-GM formation after 15 days, CC50=30μM. 17329103
MRC5 Antiviral assay Antiviral activity against Human CMV T2287 in MRC5 cells by PRA, IC50=30.1μM. 17043128
HFF Antiviral assay Antiviral activity against Human CMV T2311 in HFF cells by SEAP assay, IC50=30.2μM. 17043128
neural precursor cells Function assay Inhibition of neurosphere proliferation of mouse neural precursor cells by MTT assay 17417631
SJ-GBM2 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SJ-GBM2 cells 29435139
A673 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for A673 cells 29435139
SK-N-MC qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SK-N-MC cells 29435139
BT-37 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-37 cells 29435139
Saos-2 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Saos-2 cells 29435139
BT-12 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-12 cells 29435139
OHS-50 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for OHS-50 cells 29435139
MG 63 (6-TG R) qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for MG 63 (6-TG R) cells 29435139
Rh41 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Rh41 cells 29435139
HFF Antiviral assay 6 hrs Antiviral activity against pp28-luciferase expressing HCMV Towne infected in human HFF cells assessed as reduction in viral replication treated with compound immediately after viral infection at MOI of 1 PFU/cell followed by compound washout after 6 hrs a 32191456
HFF Antiviral assay 5 uM Antiviral activity against HCMV Towne infected in human HFF cells assessed as reduction in viral DNA yields in the supernatant at 5 uM treated with compound following viral infection at MOI of 0.1 PFU/cell by US17 real time PCR analysis 32191456
HFF Antiviral assay 5 uM Antiviral activity against HCMV Towne infected in human HFF cells assessed as reduction in viral DNA replication at 5 uM treated with compound following viral infection at MOI of 0.1 PFU/cell by US17 real time PCR analysis 32191456
Vero Function assay 10 uM Inhibition of UL42 gene transcription in HSV-1 infected in African green monkey Vero cells at 10 uM by qRT-PCR analysis 31503476
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Biological Activity

Description Ganciclovir is an antiviral drug for feline herpesvirus type-1 with IC50 of 5.2 μM in a cell-free assay.
In vitro
In vitro Ganciclovir is metabolized to the triphosphate form by primarily three cellular enzymes: (1) a deoxyguanosine kinase induced by CMV-infected cells; (2) guanylate kinase; and (3) phosphoglycerate kinase. [1] Ganciclovir is sufficient to induce cell death in most bystander cells cocultured with HSV-tk-expressing cells. [2] Ganciclovir significantly reduces DNA synthesis in the transformed cells, whereas Ganciclovir has little effect on DNA synthesis in the nontransformed cells. Ganciclovir exhibits a concentration-dependent reduction in the rate of elongation into mature DNA. [3] Ganciclovir induces cell cycle arrests rather than direct chemical effect on HSVtk-transduced B16F10 melanoma cells. [4] Ganciclovir produces only weak inhibition of DNA synthesis. Ganciclovir-treated cells accumulate in early S-phase and remained there until cell death, suggesting that ganciclovir incorporation in the DNA template is important for cytotoxicity. [5] Ganciclovir-induced apoptosis is due to incorporation of the drug into DNA resulting in replication-dependent formation of DNA double-strand breaks and, at later stages, S and G2/M arrest. Ganciclovir-provoked DNA instability is likely to be responsible for the observed initial decline in Bcl-2 level and caspase-9/-3 activation. [6]
Cell Research Cell lines BV-2 cells 
Concentrations --
Incubation Time 24 h
Method

To assess cell proliferation by thymidine incorporation, a total of 2 × 10<sup>4</sup> BV-2 cells were seeded in a 96-well plate for a maximum of 12 h in 10% FBS containing DMEM and then serum starved for an additional 12 h before the addition of varying concentrations of GCV for a total of 24 h. Cultures were pulsed for the final 8 h with 1 µCi/well [3H]thymidine before incorporated radioactivity was measured using a β plate scintillation counter.
Experimental Result Images Methods Biomarkers Images PMID
Growth inhibition assay Cell viability 29845211
In Vivo
In vivo Antiviral drug ganciclovir (GCV) inhibits the proliferation of microglia in experimental autoimmune encephalomyelitis (EAE). GCV attenuates neuroinflammation and does not significantly restrain the peripheral immune response[7].
Animal Research Animal Models C57BL/6 mice
Dosages 100 mg/kg
Administration i.p.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT06407232 Not yet recruiting
Cytomegalovirus Infections|Kidney Transplant Infection|Pancreas Transplant
University of Wisconsin Madison|Merck Sharp & Dohme LLC
 May 2024 Phase 3
NCT06057194 Not yet recruiting
Infections Cytomegalovirus
Maimónides Biomedical Research Institute of Córdoba|MERCK SHARP & DOHME DE ESPAÑA S.A.
 October 2023 Phase 2
NCT04706507 Recruiting
Acute Respiratory Failure
Fred Hutchinson Cancer Center|National Heart Lung and Blood Institute (NHLBI)
 June 29 2021 Phase 3
NCT04478474 Completed
Cytomegalovirus Infections
New York Medical College|Merck Sharp & Dohme LLC
 September 15 2020 --
NCT03698435 Unknown status
Cytomegalovirus Infections
University Medical Center Groningen
 May 25 2018 --

Chemical Information & Solubility

Molecular Weight 255.23 Formula

C9H13N5O4
CAS No. 82410-32-0 SDF Download Ganciclovir SDF
Smiles C1=NC2=C(N1COC(CO)CO)N=C(NC2=O)N
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 51 mg/mL ( (199.81 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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