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Doxycycline HCl

Name: Doxycycline HCl
Brand: Selleck Chemicals
SKU: S4005
Rating: 5 (1 reviews)

Catalog No.S4005 Purity:99.82%

Doxycycline HCl is a tetracycline antibiotic that commonly used to treat a variety of infections, and also a MMP inhibitor.

Doxycycline HCl Chemical Structure

CAS No. 10592-13-9

Purity & Quality Control

Batch: Purity: 99.82%

99.82

Doxycycline HCl Related Products

Signaling Pathway

MMP Signaling Pathway

Biological Activity

Description

Doxycycline HCl is a tetracycline antibiotic that commonly used to treat a variety of infections, and also a MMP inhibitor.

Targets

MMP ^[1]

In vitro
In vitro	Doxycycline (50 μM) completely inhibits degradation of type II collagen, but not that of type I collagen in rheumatoid synovial fibroblasts and endothelial cells. Doxycycline (50 μM) down-regulated MMP-8 induction, at both the mRNA and protein levels in rheumatoid synovial fibroblasts and endothelial cells. ^[1] Doxycycline not only inhibits MMP-8 and MMP-9 (gelatinase B) activity, but also the synthesis of MMPs in human endothelial cells. Doxycycline (50 μM) completely inhibits the phorbol-12-myristate-13-acetate (PMA)-mediated induction of MMP-8 and MMP-9, as measured by Western blotting and gelatin zymography, respectively. ^[2] Doxycycline inhibits the LPS-induced IL-1beta increase in the mRNA and protein amounts in the corneal epithelium and upregulates the expression of the anti-inflammatory IL-1 RA protein in human cultured corneal epithelium. Doxycycline reduces the steady state level of the cellular ICE protein but does not affect the level of ICE transcripts. Doxycycline significantly decreases IL-1beta bioactivity in the supernatants from LPS-treated corneal epithelial cultures.^[3]

In Vivo
In vivo	Doxycycline (25 mg/day) prevents the structural deterioration of aortic elastin without decreasing the influx of inflammatory cells in Wistar rats. Doxycycline (25 mg/day) markedly suppresses increased aortic wall production of 92 kD gelatinase in Wistar rats. ^[4] Doxycycline reduces the activity of matrix metalloproteinase (MMP)-2 and MMP-9 in the arterial wall, and inhibits smooth muscle cell migration from media to intima by 77% at 4 days after balloon injury in rats. Doxycycline reduces the replication of smooth muscle cells in the intima in rats. ^[5]

References

[4] Fung HB, et al. Clin Ther, 2005, 27(12), 1859-1884.

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Chemical Information & Solubility

Molecular Weight	480.9	Formula	C₂₂H₂₄N₂O₈.HCl
CAS No.	10592-13-9	SDF	Download Doxycycline HCl SDF
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 96 mg/mL ( (199.62 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molecular Weight Calculator

In vivo Batch: Add solvents to the product individually and in order.					In vivo Formulation Calculator
	Clear solution	5% DMSO 1 95% Corn oil	0.6mg/ml (1.25mM)	Taking the 1 mL working solution as an example, add 50 μL of 12 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.	In vivo Formulation Calculator
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	4.8mg/ml (9.98mM)	Taking the 1 mL working solution as an example, add 50 μL of 96 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	4.8mg/ml (9.98mM)	Taking the 1 mL working solution as an example, add 50 μL of 96 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil	0.6mg/ml (1.25mM)	Taking the 1 mL working solution as an example, add 50 μL of 12 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

Preparing Stock Solutions

Molarity Calculator

Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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