Home Proteases MMP inhibitor Doxycycline HCl For research use only.

Doxycycline HCl

Doxycycline HCl is a tetracycline antibiotic that commonly used to treat a variety of infections, and also a MMP inhibitor.

Doxycycline HCl Chemical Structure

Doxycycline HCl Chemical Structure

CAS No. 10592-13-9

Purity & Quality Control

Doxycycline HCl Related Products

Signaling Pathway

MMP Signaling Pathways

MMP Signaling Pathway

Biological Activity

Description Doxycycline HCl is a tetracycline antibiotic that commonly used to treat a variety of infections, and also a MMP inhibitor.
Targets
MMP [1]
In vitro
In vitro Doxycycline (50 μM) completely inhibits degradation of type II collagen, but not that of type I collagen in rheumatoid synovial fibroblasts and endothelial cells. Doxycycline (50 μM) down-regulated MMP-8 induction, at both the mRNA and protein levels in rheumatoid synovial fibroblasts and endothelial cells. [1] Doxycycline not only inhibits MMP-8 and MMP-9 (gelatinase B) activity, but also the synthesis of MMPs in human endothelial cells. Doxycycline (50 μM) completely inhibits the phorbol-12-myristate-13-acetate (PMA)-mediated induction of MMP-8 and MMP-9, as measured by Western blotting and gelatin zymography, respectively. [2] Doxycycline inhibits the LPS-induced IL-1beta increase in the mRNA and protein amounts in the corneal epithelium and upregulates the expression of the anti-inflammatory IL-1 RA protein in human cultured corneal epithelium. Doxycycline reduces the steady state level of the cellular ICE protein but does not affect the level of ICE transcripts. Doxycycline significantly decreases IL-1beta bioactivity in the supernatants from LPS-treated corneal epithelial cultures.[3]
In Vivo
In vivo Doxycycline (25 mg/day) prevents the structural deterioration of aortic elastin without decreasing the influx of inflammatory cells in Wistar rats. Doxycycline (25 mg/day) markedly suppresses increased aortic wall production of 92 kD gelatinase in Wistar rats. [4] Doxycycline reduces the activity of matrix metalloproteinase (MMP)-2 and MMP-9 in the arterial wall, and inhibits smooth muscle cell migration from media to intima by 77% at 4 days after balloon injury in rats. Doxycycline reduces the replication of smooth muscle cells in the intima in rats. [5]

Chemical Information & Solubility

Molecular Weight 480.9 Formula

C22H24N2O8.HCl
CAS No. 10592-13-9 SDF Download Doxycycline HCl SDF
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 96 mg/mL ( (199.62 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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