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Catalpol

Synonyms: Catalpinoside, Digitalis purpurea L

Catalpol (Catalpinoside, Digitalis purpurea L), an iridoid glucoside contained richly in the roots of the small flowering plant species Rehmannia glutinosa Libosch, has been shown to have antioxidation, anti-inflammation, anti-apoptosis and other neuroprotective properties and plays a role in neuroprotection against hypoxic/ischemic injury, AD and PD in both in vivo and in vitro models.

Catalpol Chemical Structure

Catalpol Chemical Structure

CAS No. 2415-24-9

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Catalpol Related Products

Biological Activity

Description Catalpol (Catalpinoside, Digitalis purpurea L), an iridoid glucoside contained richly in the roots of the small flowering plant species Rehmannia glutinosa Libosch, has been shown to have antioxidation, anti-inflammation, anti-apoptosis and other neuroprotective properties and plays a role in neuroprotection against hypoxic/ischemic injury, AD and PD in both in vivo and in vitro models.
In vitro
In vitro Catalpol is able to inhibit HCT116 cell proliferation. Furthermore, catalpol induces apoptosis in HCT116 cells, which depends on the increased activities of caspase-3 and −9. In addition, catalpol reduces the expression of PI3K, p-Akt and Akt in HCT116 cells[1]. In vitro, catalpol inhibits TNF-α, IL-6, IL-4 and IL-1β production and up-regulates IL-10 expression in LPS-stimulated alveolar macrophages[2].
Cell Research Cell lines HCT116 cells
Concentrations 0, 25, 50 and 100 µg/ml
Incubation Time 24, 48, 72 h
Method HCT116 cells (2×104 cells/well) are seeded in 96-well plates and cell viability is detected using MTT. HCT116 cells are cultured with various concentrations of catalpol (0, 25, 50 and 100 µg/ml). Following treatment for 24, 48, and 72 h, 20 µl MTT solution (0.5 mg/ml) is added into each well and cells are incubated at 37°C for 4 h. The culture medium of each well is subsequently removed and 150 µl dimethyl sulfoxide is added into each well at room temperature whilst being shaken for 20 min. Absorbance is measured at 570 nm using a Bio-Rad ELISA reader.
In Vivo
In vivo Catalpol decreases peroxynitrite formation and consequently exerts cardioprotective effects through the PI3K/Akt signaling pathway in ischemic/reperfusion rats. Catalpol inhibits lung W/D ratio, myeloperoxidase activity of lung samples, the amounts of inflammatory cells and TNF-α, IL-6, IL-4 and IL-1β in BALF induced by LPS. The production of IL-10 in BALF is up-regulated by catalpol[2].
Animal Research Animal Models Mice acute lung injury model (Male BALB/c mice)
Dosages 2.5, 5 and 10 mg/kg
Administration i.p.

Chemical Information & Solubility

Molecular Weight 362.33 Formula

C15H22O10
CAS No. 2415-24-9 SDF --
Smiles C1=COC(C2C1C(C3C2(O3)CO)O)OC4C(C(C(C(O4)CO)O)O)O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 72 mg/mL ( (198.71 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 72 mg/mL

Ethanol : 5 mg/mL


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In vivo
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