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bisBenzimide H 33258 Trihydrochloride

Synonyms: Hoechst 33258 trihydrochloride; H 33258 trihydrochloride

bisBenzimide H 33258 Trihydrochloride(Hoechst 33258 trihydrochloride; H 33258 trihydrochloride) is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. It binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity. Therefore, Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution. It exhibits anti-tumor activity IC50 of 51.31±4.56 μM in HeLa cells.

bisBenzimide H 33258 Trihydrochloride Chemical Structure

bisBenzimide H 33258 Trihydrochloride Chemical Structure

CAS No. 23491-45-4

Purity & Quality Control

Batch: E291001 DMSO]31 mg/mL]false]Water]23 mg/mL]false]Ethanol]Insoluble]false Purity: 99.60%
99.60

bisBenzimide H 33258 Trihydrochloride Related Products

Biological Activity

Description bisBenzimide H 33258 Trihydrochloride(Hoechst 33258 trihydrochloride; H 33258 trihydrochloride) is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. It binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity. Therefore, Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution. It exhibits anti-tumor activity IC50 of 51.31±4.56 μM in HeLa cells.

Chemical Information & Solubility

Molecular Weight 533.88 Formula

C25H24N6O.3ClH
CAS No. 23491-45-4 SDF --
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 31 mg/mL ( (58.06 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 23 mg/mL

Ethanol : Insoluble


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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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