Home PI3K/Akt/mTOR AMPK activator - Autophagy activator BC1618 For research use only.

BC1618

BC1618 is an orally active Fbxo48 inhibitor that stimulates adenosine monophosphate (AMP)-activated protein kinase (Ampk)-dependent signaling. BC1618 promotes mitochondrial fission, facilitates autophagy and improves hepatic insulin sensitivity.

BC1618 Chemical Structure

BC1618 Chemical Structure

CAS No. 2222094-18-8

Purity & Quality Control

Batch: S986001 DMSO]83 mg/mL]false]Ethanol]83 mg/mL]false]Water]Insoluble]false Purity: 99.99%
99.99

BC1618 Related Products

Signaling Pathway

AMPK Signaling Pathways

AMPK Signaling Pathway

Biological Activity

Description BC1618 is an orally active Fbxo48 inhibitor that stimulates adenosine monophosphate (AMP)-activated protein kinase (Ampk)-dependent signaling. BC1618 promotes mitochondrial fission, facilitates autophagy and improves hepatic insulin sensitivity.
Targets
Fbxo48 [1]
In vitro
In vitro

BC1618 interrupts Fbxo48/pAmpkα interaction. BC1618 facilitates mitochondrial fission and autophagy.[1]
Cell Research Cell lines 293T cells
Concentrations 3 μM
Incubation Time 30 min
Method

293T cells transfected with Fbxo48-V5 or Fbxo30-V5 are treated with control (DMSO) or BC1618 (3 μM) for 30 min. Cells are then washed and equally fractioned into 10 aliquots and subjected to a range of increasing temperatures to promote protein denaturation. After a high-speed spin to precipitate heat-denatured proteins, the soluble protein fraction from each temperature is then immunoblotted for Fbxo48 or Fbxo30.
In Vivo
In vivo

Consistent with augmenting Ampk activity, BC1618 promotes mitochondrial fission, facilitates autophagy and improves hepatic insulin sensitivity in high-fat-diet-induced obese mice.[1]
Animal Research Animal Models C57BL/6 mice
Dosages 15 mg/kg, 20 mg/kg, 30 mg/kg
Administration Oral gavage

Chemical Information & Solubility

Molecular Weight 415.45 Formula

C24H24F3NO2
CAS No. 2222094-18-8 SDF --
Smiles OC(COC1=CC=C(C=C1)C(F)(F)F)CN(CC2=CC=CC=C2)CC3=CC=CC=C3
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 83 mg/mL ( (199.78 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 83 mg/mL

Water : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy BC1618 | BC1618 supplier | purchase BC1618 | BC1618 cost | BC1618 manufacturer | order BC1618 | BC1618 distributor