Home Epigenetics SMYD inhibitor AZ505 For research use only.

AZ505

AZ505 is a potent and selective inhibitor of SMYD2 with an IC50 of 0.12 μM.

AZ505 Chemical Structure

AZ505 Chemical Structure

CAS No. 1035227-43-0

Purity & Quality Control

Batch: S704201 DMSO]100 mg/mL]false]Ethanol]2 mg/mL]false]Water]Insoluble]false Purity: 99.99%
99.99

AZ505 Related Products

Biological Activity

Description AZ505 is a potent and selective inhibitor of SMYD2 with an IC50 of 0.12 μM.
Targets
SMYD2 [1]
0.12 μM
In vitro
In vitro

AZ505 inhibits the binding of SMYD2 to the miR-125b promoter region (based on chromatin immunoprecipitation assays) and suppresses clear cell renal cell carcinoma (ccRCC) cells migration and invasion by inhibiting the SMYD2/miR-125b/DKK3 pathway. <sup><a class="sref" href="#s_ref">[2]</a></sup>
Cell Research Cell lines OSRC-2cells, 786-O cells
Concentrations 0-1.5 mM
Incubation Time 12 h
Method

To evaluate the differentially expressed miRNAs in AZ505-treated and siSMYD2-transfected ccRCC cells relative to control cells, siSMYD-, AZ505-, and negative control-treated 786-O cells are subjected to miRNA microarray, using the miRCURY LNA Array system. And then ChIP is performed using the EZ-Chromatin Immunoprecipitation Kit.
In Vivo
In vivo

AZ505 treatment resulted in the decrease of the tumor sizes and weights in the renal cell carcinoma xenograft model mice. <sup><a class="sref" href="#s_ref">[2]</a></sup>
Animal Research Animal Models BALB/c nude mice bearing with 786-O cells
Dosages 40 mg/kg
Administration i.p.

Chemical Information & Solubility

Molecular Weight 577.54 Formula

C29H38Cl2N4O4
CAS No. 1035227-43-0 SDF --
Smiles OC1=C2NC(=O)COC2=C(CCNCCN(C3CCCCC3)C(=O)CCNCCC4=CC(=C(Cl)C=C4)Cl)C=C1
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 100 mg/mL ( (173.14 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 2 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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