Home Metabolism phosphatase inhibitor BVT-948 For research use only.

BVT-948

BVT 948 is a irreversible, noncompetitive and cell-permeable protein tyrosine phosphatase（PTP） inhibitor with IC50s of 0.9 μM, 1.7 μM, 0.09 μM, 1.5 μM and 0.7 μM for PTP1B, TCPTP, SHP-2, LAR and YopH respectively.

BVT-948 Chemical Structure

BVT-948 Chemical Structure

CAS No. 39674-97-0

Purity & Quality Control

Batch: S982901 DMSO]48 mg/mL]false]Ethanol]5 mg/mL]false]Water]Insoluble]false Purity: 99.77%
99.77

BVT-948 Related Products

Biological Activity

Description BVT 948 is a irreversible, noncompetitive and cell-permeable protein tyrosine phosphatase（PTP） inhibitor with IC50s of 0.9 μM, 1.7 μM, 0.09 μM, 1.5 μM and 0.7 μM for PTP1B, TCPTP, SHP-2, LAR and YopH respectively.
Targets
SHP-2 [1]
(Cell-free assay)		 YopH [1]
(Cell-free assay)		 PTP1B [1]
(Cell-free assay)		 LAR [1]
(Cell-free assay)		 TCPTP [1]
(Cell-free assay)
0.09 μM 0.7 μM 0.9 μM 1.5 μM 1.7 μM
In vitro
In vitro

In L6 myotubes, PTP1B is oxidized further to the irreversible sulfinic and sulfonic forms with BVT.948. BVT.948 is able to increase tyrosine phosphorylation of cellular proteins, including the insulin receptor. BVT.948 inhibits several other PTPs tested and also was efficient at inhibiting several cytochrome P450 (P450) isoforms.[1]
Cell Research Cell lines L6 myotubes
Concentrations 25 μM
Incubation Time 30 min
Method

L6 myotubes are starved of serum overnight and stimulated with insulin or treated with 25 M BVT.948 after a 30-min preincubation with (dark gray bars) or without (light gray bars) 100 nM wortmannin. After washing, glucose uptake was measured.
In Vivo
In vivo

BVT.948 enhances insulin tolerance tests in ob/ob mice, therefore apparently enhancing insulin sensitivity. BVT.948 can significantly enhance glucose clearance from the blood stream in response to insulin. [1]
Animal Research Animal Models ob/ob mice
Dosages 0.3 μmol/kg, 3 μmol/kg
Administration i.p.

Chemical Information & Solubility

Molecular Weight 241.24 Formula

C14H11NO3
CAS No. 39674-97-0 SDF --
Smiles CC1(C)C(=O)N=C2C3=CC=CC=C3C(=O)C(=C12)O
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 48 mg/mL ( (198.97 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 5 mg/mL

Water : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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