Z-VAD(OH)-FMK (Caspase Inhibitor VI)

Catalog No.S8102 Batch:S810201

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Technical Data

Formula

C21H28FN3O7
Molecular Weight 453.46 CAS No. 161401-82-7
Solubility (25°C)* In vitro DMSO 90 mg/mL (198.47 mM)
Ethanol 90 mg/mL (198.47 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
2%DMSO 40%PEG300 5%Tween80 53%ddH2O
0.2mg/ml Taking the 1 mL working solution as an example, add 20 μL of clarified DMSO stock solution of 10 mg/ml to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify it; then continue to add 530 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Z-VAD(OH)-FMK (Caspase Inhibitor VI) is an irreversible pan-caspase inhibitor.
Targets
Caspase [1]
In vitro

Z-VAD(OH)-FMK is the non-methylated form of Z-VAD-FMK, and useful in studies including recombinant, or purified enzymes. [1]
In vivo

Z-VAD-FMK is a widely used broad-spectrum caspase inhibitor that protects muscle from compression-induced damage and restores muscle function.

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    Panc-1 cells

  • Concentrations

    50 μM

  • Incubation Time

    1 h

  • Method

    Cells were treated with indicated concentration of the drug for an hour.
Animal Study:

[3]
  • Animal Models

    Male BALB/C mice

  • Dosages

    1.5 mg/kg

  • Administration

    s.c.

Customer Product Validation

Data from [Data independently produced by , , Med Oncol, 2015, 32(4):100. ]

Selleck's Z-VAD(OH)-FMK (Caspase Inhibitor VI) has been cited by 27 publications

STING orchestrates the neuronal inflammatory stress response in multiple sclerosis [ Cell, 2024, 187(15):4043-4060.e30] PubMed: 38878778
Prostaglandin E2-EP2/EP4 signaling induces immunosuppression in human cancer by impairing bioenergetics and ribosome biogenesis in immune cells [ Nat Commun, 2024, 15(1):9464] PubMed: 39487111
Si-Wu-Tang attenuates hepatocyte PANoptosis and M1 polarization of macrophages in non-alcoholic fatty liver disease by influencing the intercellular transfer of mtDNA [ J Ethnopharmacol, 2024, 328:118057] PubMed: 38518965
Unraveling Hematotoxicity of α-Amanitin in Cultured Hematopoietic Cells [ Toxins (Basel), 2024, 16(1)61] PubMed: 38276537
Intracellular biliverdin dynamics during ferroptosis [ J Biochem, 2024, mvae067] PubMed: 39340324
The centrosomal protein 131 participates in the regulation of mitochondrial apoptosis [ Commun Biol, 2023, 6(1):1271] PubMed: 38102401
Identification of a group of bisbenzylisoquinoline (BBIQ) compounds as ferroptosis inhibitors [ Cell Death Dis, 2022, 13(11):1000] PubMed: 36435804
Mercuric chloride induces sequential activation of ferroptosis and necroptosis in chicken embryo kidney cells by triggering ferritinophagy [ Free Radic Biol Med, 2022, S0891-5849(22)00222-2] PubMed: 35675856
Physapruin A Enhances DNA Damage and Inhibits DNA Repair to Suppress Oral Cancer Cell Proliferation [ Int J Mol Sci, 2022, 23(16)8839] PubMed: 36012104
Inhibition of PI3K/AKT signaling via ROS regulation is involved in Rhein-induced apoptosis and enhancement of oxaliplatin sensitivity in pancreatic cancer cells [ Int J Biol Sci, 2021, 17(2):589-602] PubMed: 33613115

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.