Z-IETD-FMK

Catalog No.S7314 Batch:S731401

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Technical Data

Formula

C30H43FN4O11
Molecular Weight 654.68 CAS No. 210344-98-2
Solubility (25°C)* In vitro DMSO 91 mg/mL (138.99 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Z-IETD-FMK (Caspase-8 Inhibitor, Z-IE(OMe)TD(OMe)-FMK) is a specific Caspase-8 inhibitor. Z-IETD-FMK is also an inhibitor of granzyme B.
Targets
Caspase-8 [1]
In vitro

Z-IETD-FMK, which inhibits the cleavage of caspase-8 and partially inhibits the cleavage of caspase-3 and PARP, prevents the execution of apoptosis in retinal cells exposed to different apoptotic stimuli. [1]

Z-IETD-FMK (50 μM) reduces ceramide-induced cardiomyocyte death and significantly inhibits the activation of caspase 3. [2]

Inhibition of caspase-8 by Z-IETD-FMK affects the generation of activated/memory T cells and T cell cytokine production, and decreases NF-kappaB responses to TCR:CD3 engagement in T cell cultures. [3]
In vivo

In vivo, inhibition of caspase-8 by Z-IETD-FMK reduces memory/activated CD4 and CD8 T cells, and increases susceptibility to T. cruzi infection. [3]

Z-IETD-FMK promotes neuronal survival and regeneration of injured retinal ganglion cells after CNS injuries. [4]

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    Rat neonatal cardiomyocytes

  • Concentrations

    50 µM

  • Incubation Time

    24 h

  • Method

    Drugs were added to the cell culture medium simultaneously with ceramide, and surviving cells were counted 24 hours later.
Animal Study:

[3]
  • Animal Models

    T. cruzi-infected mice

  • Dosages

    0.4 mg/3 days

  • Administration

    --

Customer Product Validation

Data from [Data independently produced by , , Cell Physiol Biochem, 2017, 42(5):1822-1836]

Data from [Data independently produced by , , Int J Oncol, 2018, 52(3):1023-1032]

Data from [Data independently produced by , , Drug Des Devel Ther, 2017, 11:2841-2850]

Data from [Data independently produced by , , PLoS One, 2017, 12(2):e0171840]

Selleck's Z-IETD-FMK has been cited by 73 publications

EFHD2 suppresses intestinal inflammation by blocking intestinal epithelial cell TNFR1 internalization and cell death [ Nat Commun, 2024, 15(1):1282] PubMed: 38346956
PARP inhibitors enhance antitumor immune responses by triggering pyroptosis via TNF-caspase 8-GSDMD/E axis in ovarian cancer [ J Immunother Cancer, 2024, 12(10)e009032] PubMed: 39366751
RIPK1 is dispensable for cell death regulation in β-cells during hyperglycemia [ Mol Metab, 2024, 87:101988] PubMed: 39004142
Interferon alpha promotes caspase-8 dependent ultraviolet light-mediated keratinocyte apoptosis via interferon regulatory factor 1 [ Front Immunol, 2024, 15:1384606] PubMed: 38660315
Receptor-interacting protein kinase 1 confers autophagic promotion of gasdermin E-mediated pyroptosis in aristolochic acid-induced acute kidney injury [ Ecotoxicol Environ Saf, 2024, 284:116944] PubMed: 39208575
The mechanosensitive Piezo1 channel exacerbates myocardial ischaemia/reperfusion injury by activating caspase-8-mediated PANoptosis [ Int Immunopharmacol, 2024, 139:112664] PubMed: 39008937
ERK mediates interferon gamma-induced melanoma cell death [ Mol Cancer, 2023, 10.1186/s12943-023-01868-x] PubMed: 37803324
THADA inhibition in mice protects against type 2 diabetes mellitus by improving pancreatic β-cell function and preserving β-cell mass [ Nat Commun, 2023, 14(1):1020] PubMed: 36823211
Caspase-8 inhibition improves the outcome of bacterial infections in mice by promoting neutrophil activation [ Cell Rep Med, 2023, 4(7):101098] PubMed: 37390829
Breast tumors interfere with endothelial TRAIL at the premetastatic niche to promote cancer cell seeding [ SCIENCE ADVANCES, 2023, Vol-9,-Issue-12] PubMed: None

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.