Yoda1

Catalog No.S6678 Batch:S667801

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Technical Data

Formula

C13H8Cl2N4S2
Molecular Weight 355.27 CAS No. 448947-81-7
Solubility (25°C)* In vitro DMSO 10 mg/mL (28.14 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Yoda1 acts as an agonist for both human and mouse Piezo1(EC50 of 17.1 and 26.6 μM for mouse and human). Yoda1 activates purified Piezo1 channels in the absence of other cellular components.
Targets
mPiezo1 [1]
(Cell-free assay)		 hPiezo1 [1]
(Cell-free assay)
17.1 μM(EC50) 26.6 μM(EC50)
In vitro

Yoda-1 dramatically increases the YAP expression and ECM secretion reduced by Piezo1 silencing in vitro.[2]

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    Immortalized mouse renal MCs (SV40-MES-13)

  • Concentrations

    --

  • Incubation Time

    48 h

  • Method

    Immortalized mouse renal MCs (SV40-MES-13) were cultured in DMEM/F12 containing 5% fetal bovine serum (FBS), 0.5% penicillin and streptomycin in an atmosphere of 5% CO2 at 37 ℃. Following overnight serum deprivation, the SV40-MES-13 cells are seeded on cover slips coated with either 2 kPa (soft) or 50 kPa (stiff) hydrogels, and then treated with the YAP inhibitor Super-TDU, Piezo1 activator Yoda-1, MEK1/2 inhibitor PD0325901, JNK inhibitor SP600125, or the p38 kinase inhibitor SB203580 for 48 h.

Selleck's Yoda1 has been cited by 15 publications

Ion channel Piezo1 activation aggravates the endothelial dysfunction under a high glucose environment [ Cardiovasc Diabetol, 2024, 23(1):150] PubMed: 38702777
Low-intensity pulsed ultrasound promotes the osteogenesis of mechanical force-treated periodontal ligament cells via Piezo1 [ Front Bioeng Biotechnol, 2024, 12:1347406] PubMed: 38694622
Low-intensity pulsed ultrasound promotes the osteogenesis of mechanical force-treated periodontal ligament cells via Piezo1 [ Front Bioeng Biotechnol, 2024, 12:1347406] PubMed: 38694622
Activation of Piezo1 increases the sensitivity of breast cancer to hyperthermia therapy [ Open Med (Wars), 2024, 19(1):20240898] PubMed: 38463518
Prolonged Piezo1 Activation Induces Cardiac Arrhythmia [ Int J Mol Sci, 2023, 24(7)6720] PubMed: 37047693
Prolonged Piezo1 Activation Induces Cardiac Arrhythmia [ Int J Mol Sci, 2023, 24(7)6720] PubMed: 37047693
Inhibition of Piezo1 ameliorates intestinal inflammation and limits the activation of group 3 innate lymphoid cells in experimental colitis [ J Innate Immun, 2023, 15(1):709-23] PubMed: 37725937
Activation of Piezo1 promotes osteogenic differentiation of aortic valve interstitial cell through YAP-dependent glutaminolysis [ Sci Adv, 2023, 9(22):eadg0478] PubMed: 37267365
Piezo1-mediated mechanosensation in bone marrow macrophages promotes vascular niche regeneration after irradiation injury [ Theranostics, 2022, 12(4): 1621-1638] PubMed: N/A
Piezo1-mediated mechanosensation in bone marrow macrophages promotes vascular niche regeneration after irradiation injury [ Theranostics, 2022, 12(4):1621-1638] PubMed: 35198061

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.