Wogonin

Catalog No.S4743 Batch:S474302

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Technical Data

Formula

C16H12O5
Molecular Weight 284.26 CAS No. 632-85-9
Solubility (25°C)* In vitro DMSO 57 mg/mL (200.52 mM)
Ethanol 4 mg/mL (14.07 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Wogonin (Vogonin), a natural and biologically-active flavonoid found in plants, is an inhibitor of CDK9 and does not inhibit CDK2, CDK4 and CDK6 at doses that inhibit CDK9 activity; Also inhibits N-acetyltransferase.
Targets
CDK9 [1] N-acetyltransferase [5]
In vitro Wogonin inhibits PMA-induced COX-2 gene expression by inhibiting c-Jun expression and AP-1 activation in A549 cells[1]. Wogonin is an inhibitor of cyclin-dependent kinase 9 (CDK9) and block phosphorylation of the carboxy-terminal domain of RNA polymerase II at Ser. Thus, it reduces RNA synthesis and subsequently rapid downregulation of the short-lived anti-apoptotic protein myeloid cell leukemia 1 (Mcl-1) resulting in apoptosis induction in cancer cells. Wogonin directly binds to CDK9, presumably to the ATP-binding pocketa and does not inhibit CDK2, CDK4 and CDK6 at doses that inhibit CDK9 activity. Wogonin preferentially inhibits CDK9 in malignant compared with normal lymphocytes. Wogonin is a also potent anti-oxidant capable to scavenge •O2− [2]. Wogonin significantly inhibits the translocation of NFATc1 from the cytoplasm to the nucleus and its transcriptional activation activity. It also significantly inhibits osteoclast differentiation and decreases the transcription of osteoclast‑associated immunoglobulin‑like receptor, tartrate‑resistant acid phosphatase and calcitonin receptor[4]. Wogonin Inhibits N-acetyltransferase Activity[5].
In vivo Wogonin suppresses growth of human cancer xenografts in vivo. At doses lethal to tumor cells, wogonin shows no or little toxicity for normal cells and had also no obvious toxicity in animals[2]. Wogonin could induce apoptosis in murine sarcoma S180 thereby inhibiting the tumor growth both in vitro and in vivo[3]. An intraperitoneal injection of 200 mg/kg Wogonin could completely inhibit leukemia and CEM cells[4].

Protocol (from reference)

Cell Assay:[1]
  • Cell lines

    Human lung carcinoma A549 cells

  • Concentrations

    100 μM

  • Incubation Time

    6 h

  • Method

    A549 cells are culture in 24-well plate (1.2×105 cells/well) 1 day before wogonin treatment. DMSO or wogonin is added into A549 cells 1 h before PMA stimulation, and cells are incubated for another 6 h. Cells are collected by trypsin treatment and cell numbers are counted by using a hemocytometer and trypan blue exclusion method.
Animal Study:[3]
  • Animal Models

    ICR species mice

  • Dosages

    10, 20, 40 mg/kg

  • Administration

    i.v.

Customer Product Validation

Data from [Data independently produced by , , Exp Ther Med, 2015, 10(3):1066-1070]

Selleck's Wogonin has been cited by 3 publications

Identification of EZH2 as Cancer Stem Cell Marker in Clear Cell Renal Cell Carcinoma and the Anti-Tumor Effect of Epigallocatechin-3-Gallate -EGCG [ Cancers -Basel, 2022, 14-174200] PubMed: 36077742
STIM1 Regulates Endothelial Calcium Overload and Cytokine Upregulation During Sepsis [ J Surg Res, 2021, 263:236-244] PubMed: 33713955
Wogonin inhibits osteoclast differentiation by inhibiting NFATc1 translocation into the nucleus. [Geng X, et al. Exp Ther Med, 2015, 10(3):1066-1070] PubMed: 26622440

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.