VPS34-IN1

Catalog No.S7980 Batch:S798002

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Technical Data

Formula

C21H24ClN7O
Molecular Weight 425.91 CAS No. 1383716-33-3
Solubility (25°C)* In vitro DMSO 85 mg/mL (199.57 mM)
Ethanol 35 mg/mL (82.17 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5% DMSO 95% Corn oil
0.708mg/ml Taking the 1 mL working solution as an example, add 50 μL of 14.16 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
4.25mg/ml Taking the 1 mL working solution as an example, add 50 μL of 85 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Vps34-IN1 is a potent and highly selective Vps34 inhibitor with IC50 of 25 nM invitro,which does not significantly inhibit the isoforms of class I as well as class II PI3Ks. Vps34-IN1 modulates autophagy.
Targets
Vps34 [1]
(Cell-free assay)
25 nM
In vitro Administration of VPS34-IN1 to cells induces a rapid dose-dependent dispersal of a specific PtdIns(3)P-binding probe from endosome membranes, within 1min, without affecting the ability of class I PI3K to regulate Akt. It can also induce a rapid ~50-60% loss of SGK3 phosphorylation within 1min. VPS34-IN1 has no inhibition to the SGK2 isoform that does not possess a PtdIns(3)P-binding PX domain[1].

Protocol (from reference)

Customer Product Validation

Data from [Data independently produced by , , Oncotarget, 2017, 8(24):38099-38112]

Selleck's VPS34-IN1 has been cited by 31 publications

Non-vesicular phosphatidylinositol transfer plays critical roles in defining organelle lipid composition [ EMBO J, 2024, 10.1038/s44318-024-00096-3] PubMed: 38627600
An evolutionary-conserved VPS34-PIKfyve-TRPML1-Myosin II axis regulates the speed of amoeboid cell migration [ bioRxiv, 2024, 10.1101/2024.01.22.575998] PubMed: none
Class 3 PI3K coactivates the circadian clock to promote rhythmic de novo purine synthesis [ Nat Cell Biol, 2023, 25(7):975-988] PubMed: 37414850
S-acylation of p62 promotes p62 droplet recruitment into autophagosomes in mammalian autophagy [ Mol Cell, 2023, 83(19):3485-3501.e11] PubMed: 37802024
Dihydroartemisinin-induced ferroptosis in acute myeloid leukemia: links to iron metabolism and metallothionein [ Cell Death Discov, 2023, 9(1):97] PubMed: 36928207
Dihydroartemisinin-induced ferroptosis in acute myeloid leukemia: links to iron metabolism and metallothionein [ Cell Death Discov, 2023, 9(1):97] PubMed: 36928207
MEK inhibition overcomes chemoimmunotherapy resistance by inducing CXCL10 in cancer cells [ Cancer Cell, 2022, S1535-6108(21)00662-0] PubMed: 35051357
Inhibition of Vps34 and p110δ PI3K Impairs Migration, Invasion and Three-Dimensional Spheroid Growth in Breast Cancer Cells [ Int J Mol Sci, 2022, 23-169008] PubMed: 36012280
Bortezomib abrogates temozolomide-induced autophagic flux through an ATG5 dependent pathway [ Front Cell Dev Biol, 2022, 10:1022191] PubMed: 36619857
Inhibition of lipid kinase PIKfyve reveals a role for phosphatase Inpp4b in the regulation of PI(3)P-mediated lysosome dynamics through VPS34 activity [ J Biol Chem, 2022, 298(8):102187] PubMed: 35760104

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.