Vitamin E

Catalog No.S4686 Batch:S468602

Technical Data

Formula	C₂₉H₅₀O₂
Molecular Weight	430.71	CAS No.	59-02-9
Solubility (25°C)*	In vitro


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Biological Activity

Description

Vitamin E (D-alpha-Tocopherol) is a fat-soluble vitamin with potent antioxidant properties. It is a potent peroxyl radical scavenger and inhibits noncompetitively cyclooxygenase activity in many tissues, also inhibits angiogenesis and tumor dormancy through suppressing vascular endothelial growth factor (VEGF) gene transcription.

Targets

COX ^[2]

VEGFR ^[3]

In vitro

vitamin E is capable of inducing necrosis in TC-1 cells and has antioxidant effects against nitric oxide^[1].

In vivo

Vitamin E induces TC-1 cell necrosis in vivo and reduces tumor volume in TC-1 tumor-bearing mice. Vitamin E reduces myeloid derived suppressor cells(MDSCs) in tumors of TC-1 tumor-bearing mice and enhances T cell accumulation^[1].

Density

0.95 g/mL at 25 °C

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines TC-1 cells Concentrations 0, 25, 50 μM Incubation Time 18 h Method For in vitro cytotoxicity experiments, 1×10⁵ TC-1 cells per well are added to 24-well plates. Eighteen hours later, tumor cells are treated with Vitamin E (0, 25, 50 μM). After 18 hours, apoptotic (Annexin V+ and 7AAD−) and necrotic (Annexin V+ and 7AAD+) cells are measured using PE Annexin V Apoptosis Detection Kit I.
Animal Study:^{^[1]}	Animal Models C57BL/6 mice Dosages 2 mg/kg Administration i.p.

Cell Assay:^{^[1]}

Cell lines
TC-1 cells
Concentrations
0, 25, 50 μM
Incubation Time
18 h
Method
For in vitro cytotoxicity experiments, 1×10⁵ TC-1 cells per well are added to 24-well plates. Eighteen hours later, tumor cells are treated with Vitamin E (0, 25, 50 μM). After 18 hours, apoptotic (Annexin V+ and 7AAD−) and necrotic (Annexin V+ and 7AAD+) cells are measured using PE Annexin V Apoptosis Detection Kit I.

Animal Study:^{^[1]}

Animal Models
C57BL/6 mice
Dosages
2 mg/kg
Administration
i.p.

References

Selleck's Vitamin E has been cited by 11 publications

A pathological joint-liver axis mediated by matrikine-activated CD4+ T cells [ Signal Transduct Target Ther, 2024, 9(1):109]	PubMed: 38714712
A pathological joint-liver axis mediated by matrikine-activated CD4+ T cells [ Signal Transduct Target Ther, 2024, 9(1):109]	PubMed: 38714712
Folic acid prevents interferon-induced iron accumulation and ferroptosis and improves liver health in children with biliary atresia [ medRxiv, 2023, 10.1101/2023.12.01.23299102]	PubMed: none
Targeting ferroptosis suppresses osteocyte glucolipotoxicity and alleviates diabetic osteoporosis [ Bone Res, 2022, 10(1):26]	PubMed: 35260560
An organoid model of colorectal circulating tumor cells with stem cell features, hybrid EMT state and distinctive therapy response profile [ J Exp Clin Cancer Res, 2022, 41(1):86]	PubMed: 35260172
Inhibition of NPC1L1 disrupts adaptive responses of drug-tolerant persister cells to chemotherapy [ EMBO Mol Med, 2022, 14(2):e14903]	PubMed: 35023619
Mucin 1 Inhibits Ferroptosis and Sensitizes Vitamin E to Alleviate Sepsis-Induced Acute Lung Injury through GSK3β/Keap1-Nrf2-GPX4 Pathway [ Oxid Med Cell Longev, 2022, 2022:2405943]	PubMed: 35910848
Combination Treatment with Hydroxytyrosol and Vitamin E Improves NAFLD-Related Fibrosis [ Nutrients, 2022, 14(18)3791]	PubMed: 36145170
Ferroptosis Is Involved in Alcohol-Induced Cell Death in vivo and in vitro [ Biosci Biotechnol Biochem, 2020, 18;1-8]	PubMed: 32419644
R406 elicits anti-Warburg effect via Syk-dependent and -independent mechanisms to trigger apoptosis in glioma stem cells. [ Cell Death Dis, 2019, 10(5):358]	PubMed: 31043589

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.