Visomitin (SKQ1)

Catalog No.S9729 Batch:S972902

Technical Data

Formula	C₃₆H₄₂BrO₂P
Molecular Weight	617.6	CAS No.	934826-68-3
Solubility (25°C)*	In vitro	DMSO	100 mg/mL warmed with 50ºC water bath (161.91 mM)
		Ethanol	50 mg/mL warmed with 50ºC water bath (80.95 mM)
		Water	3 mg/mL warmed with 50ºC water bath (4.85 mM)
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Visomitin (SKQ1) is a novel mitochondrial-targeted antioxidant that holds promise in the treatment of inflammation associated with ocular surface diseases such as dry eye disease (DED) and corneal wounds. SkQ1 shows a TC50 of 317 nM on HCjE cells.
In vitro	Visomitin (SKQ1) is a new type of mitochondrial-targeted antioxidant, which is expected to be used to treat inflammation related to ocular surface diseases such as dry eye disease (DED) and corneal wounds. SkQ1 displays TC50 of 317 nM on HCjE cells.^[1]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines Human conjunctival epithelial (HCjE) and corneal epithelial (HCLE) cells Concentrations 0.25 nM-250 μM Incubation Time -- Method The SkQ1 stock solution was serially diluted in culture medium, ranging from 0.25 nM to 250,000 nM, 10 × range. As a control, the diluent (50% ethanol) was also serially diluted in culture medium, ranging from 0 to 0.75%, equivalent to the concentrations carrying over from the SkQ1 stock. Cellular morphology changes were observed and recorded and transferred to a linked computer that ran Control My Nikon v3.0 software for further analysis. Quantitative cell viability was determined by MTT assay protocols established in the laboratory.

Cell Assay:^{^[1]}

Cell lines
Human conjunctival epithelial (HCjE) and corneal epithelial (HCLE) cells
Concentrations
0.25 nM-250 μM
Incubation Time
--
Method
The SkQ1 stock solution was serially diluted in culture medium, ranging from 0.25 nM to 250,000 nM, 10 × range. As a control, the diluent (50% ethanol) was also serially diluted in culture medium, ranging from 0 to 0.75%, equivalent to the concentrations carrying over from the SkQ1 stock. Cellular morphology changes were observed and recorded and transferred to a linked computer that ran Control My Nikon v3.0 software for further analysis. Quantitative cell viability was determined by MTT assay protocols established in the laboratory.

References

[1] Yi Wei, et al. Ophthalmol Ther. 2019 Mar;8(1):63-73.

Selleck's Visomitin (SKQ1) has been cited by 1 publication

The extracellular matrix integrates mitochondrial homeostasis [ Cell, 2024, S0092-8674(24)00638-X]	PubMed: 38942015

The extracellular matrix integrates mitochondrial homeostasis [ Cell, 2024, S0092-8674(24)00638-X]

PubMed: 38942015

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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