Vincristine

Catalog No.S9555 Batch:S955501

Technical Data

Formula	C₄₆H₅₆N₄O₁₀
Molecular Weight	824.96	CAS No.	57-22-7
Solubility (25°C)*	In vitro	DMSO	100 mg/mL (121.21 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description	Vincristine (Leurocristine, NSC-67574, 22-Oxovincaleukoblastine) is a natural alkaloid isolated from the plant Vinca rosea Linn. with anti-tumor activity. Vincristine inhibits microtubule formation in mitotic spindle and binds to microtubule with Ki of 85 nM.
In vitro	Vincristine, a microtubule-destabilizing agent (MDA), inhibits the formation of microtubules and mitosis of the cancer cell, which is a victim of intratumoural metabolic resistance that has no important relevance with neither CYP3A4 nor CYP3A5.^[2]
In vivo	Vincristine shows the regression of the tumor in zebrafish xenografts.^[3]

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines HepG2_EV, HepG2_CYP3A4, HepG_CYP3A5, HepG2_CYP2C8 cells Concentrations 1 μM Incubation Time 24, 48 and 72 h Method HepG2 sublines (100×10⁴ cells/well) are seeded in 12-well plates and cultured until full confluence. Then, the media is removed, and the cells are exposed to 1 μM drug (docetaxel, paclitaxel or vincristine) or 0.01% DMSO (vehicle control). Samples are collected at 24, 48 and 72 h intervals using TRI Reagent, which is followed by RNA isolation. The RNA yield is assessed via a NanoDrop ND-1000 spectrophotometer, while its quality and integrity are verified by agarose gel electrophoresis.
Animal Study:^{^[3]}	Animal Models Zebrafish of glioma model Dosages 10-20 nM Administration p.o.

Cell Assay:^{^[2]}

Cell lines
HepG2_EV, HepG2_CYP3A4, HepG_CYP3A5, HepG2_CYP2C8 cells
Concentrations
1 μM
Incubation Time
24, 48 and 72 h
Method
HepG2 sublines (100×10⁴ cells/well) are seeded in 12-well plates and cultured until full confluence. Then, the media is removed, and the cells are exposed to 1 μM drug (docetaxel, paclitaxel or vincristine) or 0.01% DMSO (vehicle control). Samples are collected at 24, 48 and 72 h intervals using TRI Reagent, which is followed by RNA isolation. The RNA yield is assessed via a NanoDrop ND-1000 spectrophotometer, while its quality and integrity are verified by agarose gel electrophoresis.

Animal Study:^{^[3]}

Animal Models
Zebrafish of glioma model
Dosages
10-20 nM
Administration
p.o.

References

Selleck's Vincristine has been cited by 60 publications

Overcoming drug resistance of cancer cells by targeting the FGF1/FGFR1 axis with honokiol or FGF ligand trap [ Front Pharmacol, 2024, 15:1459820]	PubMed: 39329123
Protective effect of oxytocin on vincristine-induced gastrointestinal dysmotility in mice [ Front Pharmacol, 2024, 15:1270612]	PubMed: 38655179
Lumbrokinase Extracted from Earthworms Synergizes with Bevacizumab and Chemotherapeutics in Treating Non-Small Cell Lung Cancer by Targeted Inactivation of BPTF/VEGF and NF-κB/COX-2 Signaling [ Biomolecules, 2024, 14(7)741]	PubMed: 39062456
Hypoxia-initiated Cysteine-rich protein 61 secretion promotes chemoresistance of acute B lymphoblastic leukemia cells [ Am J Cancer Res, 2024, 14(7):3388-3403]	PubMed: 39113880
Panobinostat Synergizes with Chemotherapeutic Agents and Improves Efficacy of Standard-of-Care Chemotherapy Combinations in Ewing Sarcoma Cells [ Cancers (Basel), 2024, 16(21)3565]	PubMed: 39518006
Dual inhibition of topoisomerase II and microtubule of podophyllotoxin derivative 5p overcomes cancer multidrug resistance [ Eur J Pharmacol, 2024, 983:176968]	PubMed: 39233039
3-nitropyridine analogues as novel microtubule-targeting agents [ PLoS One, 2024, 19(11):e0307153]	PubMed: 39509402
Immuno-oncological effects of standard anticancer agents and commonly used concomitant drugs: an in vitro assessment [ BMC Pharmacol Toxicol, 2024, 25(1):25]	PubMed: 38444002
Immuno-oncological effects of standard anticancer agents and commonly used concomitant drugs: an in vitro assessment [ BMC Pharmacol Toxicol, 2024, 25(1):25]	PubMed: 38444002
Novel iminocoumarine imidazo [4, 5-b] pyridine derivatives: Design, synthesis and biological evaluation [ Chem Heterocycl Com+, 2024, Volume 60, pages 75–82]	PubMed: None

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SHIPPING AND STORAGE
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