VBIT-12

Catalog No.S8936 Batch:S893601

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Technical Data

Formula

C25H27N3O3
Molecular Weight 417.50 CAS No. 2089227-65-4
Solubility (25°C)* In vitro DMSO 83 mg/mL (198.8 mM)
Water 2 mg/mL (4.79 mM)
Ethanol Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
4.15mg/ml Taking the 1 mL working solution as an example, add 50 μL of 83 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
Clear solution
5% DMSO 95% Corn oil
1.03mg/ml Taking the 1 mL working solution as an example, add 50 μL of 20.6 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description VBIT-12 is a potent inhibitor of Voltage-dependent anion-selective channel 1 (VDAC1), directly interacting with purified VDAC1, reducing its channel conductance and preventing VDAC1 oligomerization.
Targets
VDAC1 [1]
In vitro

VBIT-12 directly interacts with purified VDAC1 and reduces its channel conductance. VBIT-12 interferes with VDAC1 oligomerization and thus with the pro-apoptotic action of VDAC1 when the levels of expression are abnormal, but have no effect on cells under physiological conditions.[2]
In vivo

The effect of VBIT-12 on induction of startle habituation and prepulse inhibition (PPI) in animals is examined via novelty suppressed feeding test, sweet preference model of anhedonia and forced swim test (FST) model for despair. VBIT-12 interacts with the Voltage-Dependent Anion Channel (VDAC) and can be used for the treatment of central nervous system (CNS) disorders and diseases.[1]

Protocol (from reference)

Animal Study:

[1]
  • Animal Models

    male 129 SVE adult mice, male BALB/c mice aged 8 weeks

  • Dosages

    20 mg/kg

  • Administration

    Oral gavage

Selleck's VBIT-12 has been cited by 6 publications

VDAC1, as a downstream molecule of MLKL, participates in OGD/R-induced necroptosis by inducing mitochondrial damage [ Heliyon, 2024, 10(1):e23426] PubMed: 38173512
The inhibition of VDAC1 oligomerization promotes pigmentation through the CaMK-CRTCs/CREB-MITF pathway [ Exp Cell Res, 2024, 434(2):113874] PubMed: 38070860
Phosphoglycerate mutase 5 aggravates alcoholic liver disease through disrupting VDAC-1-dependent mitochondrial integrity [ Int J Med Sci, 2024, 21(4):755-764] PubMed: 38464835
CircRNA Samd4 induces cardiac repair after myocardial infarction by blocking mitochondria-derived ROS output [ Mol Ther, 2022, S1525-0016-2200417-8] PubMed: 35791879
Protecting mitochondria via inhibiting VDAC1 oligomerization alleviates ferroptosis in acetaminophen-induced acute liver injury [ Cell Biol Toxicol, 2021, 10.1007/s10565-021-09624-x] PubMed: 34401974
Oleanolic Acid Improves Obesity-Related Inflammation and Insulin Resistance by Regulating Macrophages Activation [ Front Pharmacol, 2021, 12:697483] PubMed: 34393781

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.