USP25/28 inhibitor AZ1

Catalog No.S8904 Batch:S890403

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Technical Data

Formula

C17H16BrF4NO2
Molecular Weight 422.21 CAS No. 2165322-94-9
Solubility (25°C)* In vitro DMSO 84 mg/mL (198.95 mM)
Ethanol 84 mg/mL (198.95 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
4.2mg/ml Taking the 1 mL working solution as an example, add 50 μL of 84 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
Clear solution
5% DMSO 95% Corn oil
2.1mg/ml Taking the 1 mL working solution as an example, add 50 μL of 42 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description AZ1 is a potent and noncompetitive dual Inhibitors of the ubiquitin-specific protease (USP) 25/28 with IC50s of 0.62 μM and 0.7 μM in Ub-RH110 assay,respectively.
Targets
USP25 [1]
(Cell-free assay)		 USP28 [1]
(Cell-free assay)
0.62 μM 0.7 μM
In vitro

AZ1 is able to reduce cell viability across a range of cancer cell lines with EC50 values typically clustered around 20 μM.[1]

Protocol (from reference)

Selleck's USP25/28 inhibitor AZ1 has been cited by 2 publications

USP28 enables oncogenic transformation of respiratory cells, and its inhibition potentiates molecular therapy targeting mutant EGFR, BRAF and PI3K [ Mol Oncol, 2022, 10.1002/1878-0261.13217] PubMed: 35364627
Ubiquitin-specific protease 28 deubiquitinates TCF7L2 to govern the action of the Wnt signaling pathway in hepatic carcinoma [ Cancer Sci, 2022, 113-10:3463-3475] PubMed: 35880246

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.