UNC6934

Catalog No.E1059 Batch:E105901

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Technical Data

Formula

C24H21N5O4
Molecular Weight 443.46 CAS No. 2561494-77-5
Solubility (25°C)* In vitro DMSO 22 mg/mL (49.6 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description UNC6934 is a a chemical probe targeting the N-terminal PWWP (PWWP1) domain of NSD2. UNC6934 is a potent antagonist of NSD2-PWWP1 with a Kd (SPR) of 80 nM and is selective for NSD2-PWWP1 over 14 other PWWP domains including NSD3-PWWP1 UNC6934 disrupts the NSD2-PWWP1 interaction with H3K36me2 nucleosomes in U2OS cells as measured by a NanoBret assay with an IC50 of 1.09 μM.
Targets
NSD2 [1] NSD2 [1]
(in the NanoBret assay)
80 nM(Kd) 1.09 μM
In vitro

UNC6934-mediated antagonism of PWWP1 leads to accumulation of endogenous NSD2 in the nucleolus. [1]

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    U2OS cells

  • Concentrations

    5 μM

  • Incubation Time

    4 h

  • Method

    U2OS cells are treated for 4 h with DMSO vehicle control or 5 μM UNC6934. Confocal microscopy is used to observe the intracellular NSD2 and fibrillarin staining. Quantification of colocalization between NSD2 and the nucleolar marker fibrillarin as determined by Pearson correlation coefficient (PCC).

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.