TRULI

Catalog No.E1061 Batch:E106101

Technical Data

Formula

C₁₈H₁₄N₄OS

Molecular Weight

334.40

CAS No.

1424635-83-5

Solubility (25°C)*

In vitro

DMSO

67 mg/mL (200.35 mM)

Water

Insoluble

Ethanol

Insoluble

In vivo (Add solvents to the product individually and in order)

Clear solution

5%DMSO 1 Corn oil

3.35mg/ml

Taking the 1 mL working solution as an example, add 50 μL of 67 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

TRULI inhibits both Lats1 and Lats2 with a IC50 of 0.2 nM, suppresses Yap phosphorylation, induces cell proliferation in several cell lines and tissues, and promotes the initial stages of proliferative regeneration of the sensory receptors in the inner ear.

Targets

Lats2 ^[1] (in the Vitro kinase assay)	Lats1 ^[1] (in vitro kinase assay)	Lats2 ^[1] (in vitro kinase assay)
0.2 nM	0.2 nM	0.2 nM

In vitro

TRULI acts as an inhibitor of Lats kinases in vitro, suppresses Yap phosphorylation, induces cell proliferation in several cell lines and tissues, and promotes the initial stages of proliferative regeneration of the sensory receptors in the inner ear.^[1]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines MCF 10A cells, HEK293A cells Concentrations 10 μM Incubation Time 24 h, 72 h, 5 days Method For the small-molecule screen, chemical-library plates are thawed at room temperature and 0.1 μL of TRULI is placed in a well of a 384-well assay plate containing 10 μL of MCF 10A culture medium. MCF 10A cells are then plated in 40 μL of MCF 10A culture medium to achieve a final concentration of 10 μM for TRULI and 0.25% DMSO. After 24 h incubation, the fraction of the cells with nuclear Yap are determined and compared that value to the median negative-control value. For quantifying the proliferation of supporting cells, utricles are imaged at ×60, and then assembled into a composite tiling.

Cell Assay:^{^[1]}

Cell lines
MCF 10A cells, HEK293A cells
Concentrations
10 μM
Incubation Time
24 h, 72 h, 5 days
Method
For the small-molecule screen, chemical-library plates are thawed at room temperature and 0.1 μL of TRULI is placed in a well of a 384-well assay plate containing 10 μL of MCF 10A culture medium. MCF 10A cells are then plated in 40 μL of MCF 10A culture medium to achieve a final concentration of 10 μM for TRULI and 0.25% DMSO. After 24 h incubation, the fraction of the cells with nuclear Yap are determined and compared that value to the median negative-control value. For quantifying the proliferation of supporting cells, utricles are imaged at ×60, and then assembled into a composite tiling.

References

[1] Kastan N, et al. Nat Commun. 2021 May 25;12(1):3100.

Selleck's TRULI has been cited by 5 publications

Lung injury-induced activated endothelial cell states persist in aging-associated progressive fibrosis [ Nat Commun, 2024, 15(1):5449]	PubMed: 38937456
A LATS2 and ALKBH5 positive feedback loop supports their oncogenic roles [ Cell Rep, 2024, 43(4):114032]	PubMed: 38568805
Retinoic acid-induced protein 14 links mechanical forces to Hippo signaling [ EMBO Rep, 2024, 25(9):4033-4061]	PubMed: 39160347
RAMP1 Protects Hepatocytes against Ischemia-reperfusion Injury by Inhibiting the ERK/YAP Pathway [ J Clin Transl Hepatol, 2024, 12(4):357-370]	PubMed: 38638379
Cell attachment defines sensitivity to cold stress via the Hippo pathway [ Biochem Biophys Res Commun, 2024, 730:150373]	PubMed: 38996785

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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