Tropisetron

Catalog No.S5538 Batch:S553801

Technical Data

Formula	C₁₇H₂₀N₂O₂
Molecular Weight	284.35	CAS No.	89565-68-4
Solubility (25°C)*	In vitro	Water	56 mg/mL (196.94 mM)
		DMSO	10 mg/mL (35.16 mM)

* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

Tropisetron (ICS 205-930) is a 5-HT3 receptor antagonist and α7-nicotinic receptor agonist with an IC50 of 70.1 ± 0.9 nM for 5-HT3 receptor.

Targets

5-HT3 receptor ^[1]	α7 nAChR ^[1]
5.3 nM(Ki)	6.9 nM(Ki)

In vitro

Tropisetron inhibits antigen-induced proliferation and IL-2 production in human peripheral T cells. Tropisetron inhibits the signalling pathways that regulate the activation of the transcription factors NFAT, NF-κB and AP-1, which are known to play a critical role in the immune response^[2].

In vivo

Tropisetron could ameliorate the ischemic brain injury via decreasing myeloperoxidase activity and TNF-α level. Tropisetron elicits anti-inflammatory effects in experimental model of cisplatin-induced nephrotoxicity and these effects were completely 5-HT3-receptor-independent^[3].

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines Jurkat cells Concentrations 10, 25, 50 μg/ml Incubation Time 30 min Method Jurkat cells (1 × 10⁶ cells/ml) were stimulated with either TNFα (2 ng/ml) or PMA (20 ng/ml) plus ionomycin (0.5 ug/ml) for p65, JNK, ERK1 + 2, p38 and IκBα proteins in the presence or absence of tropisetron. Cells were then washed with PBS and proteins extracted in 50 μl of lysis buffer containing 0.5% NP-40. Protein concentration was determined by the Bradford assay and 30 μg of proteins were boiled in Laemmli buffer and electrophoresed in 10% SDS/polyacrylamide gels.
Animal Study:^{^[3]}	Animal Models Male albino mice Dosages 3 mg/kg Administration i.p.

Cell Assay:^{^[2]}

Cell lines
Jurkat cells
Concentrations
10, 25, 50 μg/ml
Incubation Time
30 min
Method
Jurkat cells (1 × 10⁶ cells/ml) were stimulated with either TNFα (2 ng/ml) or PMA (20 ng/ml) plus ionomycin (0.5 ug/ml) for p65, JNK, ERK1 + 2, p38 and IκBα proteins in the presence or absence of tropisetron. Cells were then washed with PBS and proteins extracted in 50 μl of lysis buffer containing 0.5% NP-40. Protein concentration was determined by the Bradford assay and 30 μg of proteins were boiled in Laemmli buffer and electrophoresed in 10% SDS/polyacrylamide gels.

Animal Study:^{^[3]}

Animal Models
Male albino mice
Dosages
3 mg/kg
Administration
i.p.

References

Selleck's Tropisetron has been cited by 2 publications

Shaoyao Decoction reduced T lymphocyte activation by regulating of intestinal flora and 5-hydroxytryptamine metabolism in ulcerative colitis [ Chin Med, 2024, 19(1):87]	PubMed: 38879471
Inhibiting 5-hydroxytryptamine receptor 3 alleviates pathological changes of a mouse model of Alzheimer's disease [ Neural Regen Res, 2023, 18(9):2019-2028]	PubMed: 36926728

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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