Triolein

Catalog No.S3590 Batch:S359002

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Technical Data

Formula

C57H104O6
Molecular Weight 885.43 CAS No. 122-32-7
Solubility (25°C)* In vitro DMSO 100 mg/mL (112.93 mM)
Ethanol 6 mg/mL (6.77 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
2.5mg/ml Taking the 1 mL working solution as an example, add 50 μL of 50 mg/ml clarified DMSO stock solution to 400 μL PEG300, mix evenly to clarify it; add 50 μL Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
Clear solution
5% DMSO 95% corn oil
5.0mg/ml Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Triolein is an inhibitor of metalloproteinase-1 (MMP-1) and reduces both the expression of IL-6 and ROS generation in irradiated keratinocytes.
Targets
MMP-1 [1] IL-6 [1] ROS [1]
In vitro

Triolein is an inhibitor of metalloproteinase-1 (MMP-1) and reduces both the expression of IL-6 and ROS generation in irradiated keratinocytes, which can be proposed as an active agent against skin photodamage.[1]
Density 0.908 g/mL

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    The immortalized human bulge stem cell line Tel‐E6E7, Human dermal fibroblasts

  • Concentrations

    20 µM

  • Incubation Time

    24 h

  • Method

    Tel‐E6E7 cells were seeded on day one at a density of 2000 cells per cm2 on 3T3‐swiss cells feeder layer in cFAD medium. On day five the medium was change to DMEM and F12 (1:1) mixture containing 2 mM glutamine, hydrocortisone (0.4 µg/ml), 2 mM penicillin/streptomycin, Epithelial Growth factor (5 ng/ml) and 10 % fetal bovine serum. On day six the cells were irradiated on PBS using a UVM‐57 UVP handheld UV lamp (302 nm Midrange) at sub‐lethal doses of 3.4 or 6.8 mJ/cm2. Immediately, PBS was replaced with medium containing triolein 20 µM. 24 hours later mRNA was extracted from cells using Trizol Reagent. Conditioned culture medium was collected and used to replace the medium of dermal fibroblasts cells plated the day before (75000 cells/cm2) in DMEM supplemented with 10% fetal bovine serum and 2 mM glutamine. After 48 hours dermal fibroblasts mRNA was extracted.

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.