T3 (Triiodothyronine)

Catalog No.S5726 Batch:S572603

Technical Data

Formula

C₁₅H₁₂I₃NO₄

Molecular Weight

650.97

CAS No.

6893-02-3

Solubility (25°C)*

In vitro

DMSO

100 mg/mL (153.61 mM)

0.1M NAOH

13 mg/mL (19.97 mM)

Water

Insoluble

In vivo (Add solvents to the product individually and in order)

Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	5.0mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil	0.5mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 10 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	T3 (Triiodothyronine) is a thyroid hormone that affects various physiological process in the body, including growth and development, metabolism, body temperature, and heart rate.This product is soluble but easy to precipitate after freezing in DMSO. 0.1 M NaOH is recommended as a stock solution.
In vitro	Triiodothyronine is one ingredient supplemented in the medium for inducing brown adipocytes differentiation.^[1]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines Brown preadipocytes Concentrations 2 nM Incubation Time 2 days Method Brown preadipocytes were isolated from perirenal adipose tissues of Chuanzhong black goats (1 day after birth). The cells were cultured in DMEM/F12 containing 10% fetal bovine serum and 2% penicillin/streptomycin and placed in an incubator (5% CO₂, 37°C). Until 100% confluence, DMEM/F12 was supplemented with 2% FBS containing 5 μg/ml of insulin, 0.5 mM IBMX, and 2 nM triiodothyronine to induce brown adipocytes differentiation. Two days later, the induced medium was changed to a maintenance medium (DMEM/F12 supplemented with 2% FBS, 5 μg/ml of insulin, and 2 nM triiodothyronine). Cells were harvested 8 days after differentiation. L-carnitine was added to the medium throughout the differentiation process and supplemented at 600 μΜ. SiAMPKα (GAAGAGAAATTCAGAACCT)15 were transfected by Lipofectamine 3000. Lipid droplets were stained using Oil Red O solution. After staining, 1 ml isopropanol was used to dissolve the oil red O dye, and the absorbance at 510 nm was detected. (Data sourced from selleck products)

Cell Assay:^{^[1]}

Cell lines
Brown preadipocytes
Concentrations
2 nM
Incubation Time
2 days
Method
Brown preadipocytes were isolated from perirenal adipose tissues of Chuanzhong black goats (1 day after birth). The cells were cultured in DMEM/F12 containing 10% fetal bovine serum and 2% penicillin/streptomycin and placed in an incubator (5% CO₂, 37°C). Until 100% confluence, DMEM/F12 was supplemented with 2% FBS containing 5 μg/ml of insulin, 0.5 mM IBMX, and 2 nM triiodothyronine to induce brown adipocytes differentiation. Two days later, the induced medium was changed to a maintenance medium (DMEM/F12 supplemented with 2% FBS, 5 μg/ml of insulin, and 2 nM triiodothyronine). Cells were harvested 8 days after differentiation. L-carnitine was added to the medium throughout the differentiation process and supplemented at 600 μΜ. SiAMPKα (GAAGAGAAATTCAGAACCT)15 were transfected by Lipofectamine 3000. Lipid droplets were stained using Oil Red O solution. After staining, 1 ml isopropanol was used to dissolve the oil red O dye, and the absorbance at 510 nm was detected.

(Data sourced from selleck products)

References

[1] Liu X, et al. FASEB J. 2022 Aug;36(8):e22461.

Selleck's T3 (Triiodothyronine) has been cited by 7 publications

CFH Haploinsufficiency and Complement Alterations in Early-Onset Macular Degeneration [ Invest Ophthalmol Vis Sci, 2024, 65(4):43]	PubMed: 38683564
Transcriptomic Analysis Reveals Fibroblast Growth Factor 11 (FGF11) Role in Brown Adipocytes in Thermogenic Regulation of Goats [ Int J Mol Sci, 2023, 24(13)10838]	PubMed: 37446019
Transcriptomic Analysis Reveals Fibroblast Growth Factor 11 (FGF11) Role in Brown Adipocytes in Thermogenic Regulation of Goats [ Int J Mol Sci, 2023, 24(13)10838]	PubMed: 37446019
LSD1 in beige adipocytes protects cardiomyocytes against oxygen and glucose deprivation [ Iran J Basic Med Sci, 2023, 26(1):30-36]	PubMed: 36594068
Maternal L-carnitine supplementation promotes brown adipose tissue thermogenesis of newborn goats after cold exposure [ FASEB J, 2022, 36(8):e22461]	PubMed: 35838582
RNA-Seq reveals miRNA role in thermogenic regulation in brown adipose tissues of goats [ BMC Genomics, 2022, 23(1):186]	PubMed: 35255830
Depletion of Toll-Like Receptor-9 Attenuates Renal Tubulointerstitial Fibrosis After Ischemia-Reperfusion Injury [ Front Cell Dev Biol, 2021, 9:641527]	PubMed: 33644078

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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