TRC051384

Catalog No.S8305 Batch:S830501

Technical Data

Formula

C₂₅H₃₁N₅O₄

Molecular Weight

465.54

CAS No.

867164-40-7

Solubility (25°C)*

In vitro

DMSO

93 mg/mL (199.76 mM)

Ethanol

5 mg/mL (10.74 mM)

Water

Insoluble

In vivo (Add solvents to the product individually and in order)

Clear solution

2%DMSO 1 20%PEG300 2 5%Tween80 3 73%ddH2O

0.9mg/ml

Taking the 1 mL working solution as an example, add 20 μL of 45 mg/ml clarified DMSO stock solution to 200 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 730 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

TRC051384 is an inducer of heat shock protein 70 (HSP70) and potent anti-inflammatory agent.

Targets

HSP70 ^[1]

In vitro

TRC051384 is a compound that induces HSPA1A/B expression and subsequent Hsp70 production via heat shock factor-1 (HSF1) activation^[2]. TRC051384 increases HSP70B mRNA expression in both HeLa and primary mixed neuronal cells. Induction of HSP70 by TRC051384 involves HSF1 activation and results in elevated chaperone and anti-inflammatory activity^[1].

In vivo

Treatment with TRC051384 significantly reduces stroke associated neuronal injury and disability in a rat model of transient ischemic stroke even when administered 8 hours post onset of ischemia. TRC051384 significantly reduces infarct size, brain edema and improves neurological deficit^[1]. TRC051384 delays thrombus formation in WT mice, the tail bleeding time still being unaltered^[2].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines HeLa cell line (CCL-2, ATCC) Concentrations 6.25 and 12.5 μM Incubation Time 4 h Method Induction of HSP70B mRNA is carried out by treatment of cells with TRC051384 (6.25 and 12.5 μM) for 4 hours duration for HeLa cell line and total RNA is isolated. For all RNA samples, cDNAs are synthesized and expression of HSP70B mRNA along with expression of 18S rRNA is monitored by real-time PCR employing ABI 7000 system.
Animal Study:^{^[1]}	Animal Models Sprague Dawley male rats Dosages 4.5 mg/kg and 9 mg/kg Administration i.p.

Cell Assay:^{^[1]}

Cell lines
HeLa cell line (CCL-2, ATCC)
Concentrations
6.25 and 12.5 μM
Incubation Time
4 h
Method
Induction of HSP70B mRNA is carried out by treatment of cells with TRC051384 (6.25 and 12.5 μM) for 4 hours duration for HeLa cell line and total RNA is isolated. For all RNA samples, cDNAs are synthesized and expression of HSP70B mRNA along with expression of 18S rRNA is monitored by real-time PCR employing ABI 7000 system.

Animal Study:^{^[1]}

Animal Models
Sprague Dawley male rats
Dosages
4.5 mg/kg and 9 mg/kg
Administration
i.p.

References

Selleck's TRC051384 has been cited by 3 publications

HSP70 attenuates compression-induced apoptosis of nucleus pulposus cells by suppressing mitochondrial fission via upregulating the expression of SIRT3 [ Exp Mol Med, 2022, 10.1038/s12276-022-00745-9]	PubMed: 35338257
HSP90 Inhibitor 17-AAG Attenuates Nucleus Pulposus Inflammation and Catabolism Induced by M1-Polarized Macrophages [ Front Cell Dev Biol, 2021, 9:796974]	PubMed: 35059401
Activation of HSP70 impedes tert-butyl hydroperoxide (t-BHP)-induced apoptosis and senescence of human nucleus pulposus stem cells via inhibiting the JNK/c-Jun pathway [ Mol Cell Biochem, 2021, 10.1007/s11010-021-04052-1]	PubMed: 33511552

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.