Topotecan

Catalog No.S9321 Batch:S932106

Technical Data

Formula

C₂₃H₂₃N₃O₅

Molecular Weight

421.45

CAS No.

123948-87-8

Solubility (25°C)*

In vitro

DMSO

84 mg/mL (199.31 mM)

Water

Insoluble

Ethanol

Insoluble

In vivo (Add solvents to the product individually and in order)

Clear solution	5% DMSO 1 95% Corn oil	0.15mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 3 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	2.1mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 42 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

Topotecan(NSC609699,Nogitecan,SKFS 104864A) is an antineoplastic agent used to treat ovarian cancer that works by inhibiting DNA topoisomerases.

Targets

Topo I ^[1]

In vitro

Topotecan exhibits inhibition of outgrowth of the highly invasive and migratory leader cells (LC) population, however, topotecan enrichs the LC subset 20% above the untreated control level.^[2]

In vivo

Topotecan (TPT), at least in part by inhibiting expression of Fli-1, significantly ameliorates lupus nephritis in NZBWF1 mice as effectively or better than cyclophosphamide (CYC).^[3]

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines LC-T2A-GFP lines Concentrations 1 μM Incubation Time 72 h Method LC-T2A-GFP lines are seeded at 1200 cells per well of a 384-well plate, adhered overnight, and treated with chemotherapeutics olaparib, rucaparib, paclitaxel, topotecan, carboplatin, cyclophosphamide, cisplatin, and doxorubicin at empirically determined clinically relevant doses (1 μM). Cells are maintained at 37 ℃ with 5% CO₂ in treatment for 72 h. Four focal areas per replicate well were imaged using 4X magnification on a bright field.
Animal Study:^{^[3]}	Animal Models NZBWF1 female mice Dosages 0.03 mg/kg, 0.1 mg/kg, 0.3mg/kg Administration i.p.

Cell Assay:^{^[2]}

Cell lines
LC-T2A-GFP lines
Concentrations
1 μM
Incubation Time
72 h
Method
LC-T2A-GFP lines are seeded at 1200 cells per well of a 384-well plate, adhered overnight, and treated with chemotherapeutics olaparib, rucaparib, paclitaxel, topotecan, carboplatin, cyclophosphamide, cisplatin, and doxorubicin at empirically determined clinically relevant doses (1 μM). Cells are maintained at 37 ℃ with 5% CO₂ in treatment for 72 h. Four focal areas per replicate well were imaged using 4X magnification on a bright field.

Animal Study:^{^[3]}

Animal Models
NZBWF1 female mice
Dosages
0.03 mg/kg, 0.1 mg/kg, 0.3mg/kg
Administration
i.p.

References

Selleck's Topotecan has been cited by 28 publications

Ubiquitin-specific protease 22 controls melanoma metastasis and vulnerability to ferroptosis through targeting SIRT1/PTEN/PI3K signaling [ MedComm (2020), 2024, 5(8):e684]	PubMed: 39135915
Identification and targeting of protein tyrosine kinase 7 (PTK7) as an immunotherapy candidate for neuroblastoma [ Cell Rep Med, 2023, 4(6):101091]	PubMed: 37343516
Identification and targeting of protein tyrosine kinase 7 (PTK7) as an immunotherapy candidate for neuroblastoma [ Cell Rep Med, 2023, 4(6):101091]	PubMed: 37343516
Temozolomide Sensitizes ARID1A-Mutated Cancers to PARP Inhibitors [ Cancer Res, 2023, 83(16):2750-2762]	PubMed: 37306706
Immune suppressive signaling regulated by latent transforming growth factor beta binding protein 1 promotes metastasis in cervical cancer [ Braz J Med Biol Res, 2023, 55:e12206]	PubMed: 36629522
FL118, acting as a 'molecular glue degrader', binds to dephosphorylates and degrades the oncoprotein DDX5 (p68) to control c-Myc, survivin and mutant Kras against colorectal and pancreatic cancer with high efficacy [ Clin Transl Med, 2022, 12(5):e881]	PubMed: 35604033
Secreted HSP90α-LRP1 Signaling Promotes Tumor Metastasis and Chemoresistance in Pancreatic Cancer [ Int J Mol Sci, 2022, 23(10)5532]	PubMed: 35628341
Adamantane-Monoterpenoid Conjugates Linked via Heterocyclic Linkers Enhance the Cytotoxic Effect of Topotecan [ Molecules, 2022, 27(11)3374]	PubMed: 35684313
Immunogenicity of small-cell lung cancer associates with STING pathway activation and is enhanced by ATR and TOP1 inhibition [ Cancer Med, 2022, 10.1002/cam4.5109]	PubMed: 35957613
SMARCA4/2 loss inhibits chemotherapy-induced apoptosis by restricting IP3R3-mediated Ca2+ flux to mitochondria [ Nat Commun, 2021, 12(1):5404]	PubMed: 34518526

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.