Thiazolyl Blue

Catalog No.S6821 Batch:S682101

Technical Data

Formula	C₁₈H₁₆BrN₅S
Molecular Weight	414.32	CAS No.	298-93-1
Solubility (25°C)*	In vitro	DMSO	21 mg/mL (50.68 mM)
		Ethanol	7 mg/mL (16.89 mM)
		Water	2 mg/mL (4.82 mM)
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Thiazolyl Blue (MTT, Tetrazolium bromide, Methylthiazolyldiphenyl-tetrazolium bromide, Methylthialazole Tetrazolium) is a cell-permeable and positively charged colorimetric agent that is widely used to detect reductive metabolism in cells and to measure cell proliferation, cytotoxicity, and apoptosis. Thiazolyl Blue (MTT) is reduced from yellow color to purple formazan in living cells.
In vitro	1.Preparation of MTT working solution Dissolve MTT by PBS to obtain 5 mg/mL of MTT. 2.Cell proliferation detection ( 96-well plates) 2.1Inoculated cells:Prepared single cell suspension to prepared with culture medium containing 10% FBS and inoculated into 96-well plate with 1000-10000 cells per well, volume of 100 uL per well. 2.2Incubated cells: 37℃. 5% CO2, Incubate for 24-72 h. 2.3Add 10 μL MTT in per well and incubate for 4 h, Discard the supernatant, For suspended cells, need centrifuge firstly. 2.4Add 100 μL DMSO, shock 10 min to dissolve the crystal completely 2.5Monitor the absorbance increase with an absorbance plate reader at OD = 562 nm.

Description

Thiazolyl Blue (MTT, Tetrazolium bromide, Methylthiazolyldiphenyl-tetrazolium bromide, Methylthialazole Tetrazolium) is a cell-permeable and positively charged colorimetric agent that is widely used to detect reductive metabolism in cells and to measure cell proliferation, cytotoxicity, and apoptosis. Thiazolyl Blue (MTT) is reduced from yellow color to purple formazan in living cells.

In vitro

1.Preparation of MTT working solution
Dissolve MTT by PBS to obtain 5 mg/mL of MTT.
2.Cell proliferation detection ( 96-well plates)
2.1Inoculated cells:Prepared single cell suspension to prepared with culture medium containing 10% FBS and inoculated into 96-well plate with 1000-10000 cells per well, volume of 100 uL per well.
2.2Incubated cells: 37℃. 5% CO2, Incubate for 24-72 h.
2.3Add 10 μL MTT in per well and incubate for 4 h, Discard the supernatant, For suspended cells, need centrifuge firstly.
2.4Add 100 μL DMSO, shock 10 min to dissolve the crystal completely
2.5Monitor the absorbance increase with an absorbance plate reader at OD = 562 nm.

Protocol (from reference)

References

[1] Berridge MV, et al. Biotechnol Annu Rev. 2005;11:127-52.

Selleck's Thiazolyl Blue has been cited by 5 publications

Stress granules affect the dual PI3K/mTOR inhibitor response by regulating the mitochondrial unfolded protein response [ Cancer Cell Int, 2024, 24(1):38]	PubMed: 38238825
Terazosin attenuates abdominal aortic aneurysm formation by downregulating Peg3 expression to inhibit vascular smooth muscle cell apoptosis and senescence [ Eur J Pharmacol, 2024, 968:176397]	PubMed: 38331337
5-Azacytidine-Mediated Modulation of the Immune Microenvironment in Murine Acute Myeloid Leukemia [ Cancers (Basel), 2022, 15(1)118]	PubMed: 36612115
TTK Protein Kinase promotes temozolomide resistance through inducing autophagy in glioblastoma [ BMC Cancer, 2022, 22(1):786]	PubMed: 35850753
Dietary Crocin is Protective in Pancreatic Cancer while Reducing Radiation-Induced Hepatic Oxidative Damage [ Nutrients, 2020, 12(6):E1901]	PubMed: 32604971

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.