Teduglutide

Catalog No.S9935 Batch:S993502

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Technical Data

Formula

C164H252N44O55S
Molecular Weight 3752.08 CAS No. 197922-42-2
Solubility (25°C)* In vitro DMSO 100 mg/mL (26.65 mM)
Water Insoluble
Ethanol Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5% DMSO 95% Corn oil
0.625mg/ml Taking the 1 mL working solution as an example, add 50 μL of 12.5 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
1.25mg/ml Taking the 1 mL working solution as an example, add 50 μL of 25 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Teduglutide (ALX-0600, Gattex, Revestive, TAK 633) is an analogue of human glucagon-like peptide-2 (GLP-2) and binds to the GLP-2 receptors. Teduglutide prolongs the intestinotrophic properties of GLP-2 in animal models.
In vitro

Teduglutide increases Caco-2 proliferation but tends to inhibit intestinal epithelial differentiation[4]. 
In vivo

In the Short bowel syndrome (SBS) situation, teduglutide not only maximizes small intestinal mucosal hypertrophy but also partially restores small intestinal epithelial function through an altered distribution of claudin-10, facilitating sodium recirculation for Na-coupled glucose transport and water absorption[3].

Protocol (from reference)

Cell Assay:

[4]
  • Cell lines

    Human Caco-2 intestinal epithelial cells

  • Concentrations

    500 nM

  • Incubation Time

    72 h

  • Method

    Caco-2 cells (10 000 cells per 96-well plate) are plated in cell culture medium for 24 hours. The next day, the cells are incubated with 500nM teduglutidein serum-free medium for 72 hours. Then the cells are harvested for cell proliferation assay, bromodeoxyuridine incorporation assay and cell cycle analysis.
Animal Study:

[3]
  • Animal Models

    Short bowel syndrome model mice (C57BL6/J; male)

  • Dosages

    0.1 mg/kg

  • Administration

    i.p.

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.