Teduglutide

Catalog No.S9935 Batch:S993501

Technical Data

Formula	C₁₆₄H₂₅₂N₄₄O₅₅S
Molecular Weight	3752.08	CAS No.	197922-42-2
Solubility (25°C)*	In vitro	DMSO	100 mg/mL (26.65 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Teduglutide (ALX-0600, Gattex, Revestive, TAK 633) is an analogue of human glucagon-like peptide-2 (GLP-2) and binds to the GLP-2 receptors. Teduglutide prolongs the intestinotrophic properties of GLP-2 in animal models.
In vitro	Teduglutide increases Caco-2 proliferation but tends to inhibit intestinal epithelial differentiation^[4].
In vivo	In the Short bowel syndrome (SBS) situation, teduglutide not only maximizes small intestinal mucosal hypertrophy but also partially restores small intestinal epithelial function through an altered distribution of claudin-10, facilitating sodium recirculation for Na-coupled glucose transport and water absorption^[3].

Protocol (from reference)

Cell Assay:^{^[4]}	Cell lines Human Caco-2 intestinal epithelial cells Concentrations 500 nM Incubation Time 72 h Method Caco-2 cells (10 000 cells per 96-well plate) are plated in cell culture medium for 24 hours. The next day, the cells are incubated with 500nM teduglutidein serum-free medium for 72 hours. Then the cells are harvested for cell proliferation assay, bromodeoxyuridine incorporation assay and cell cycle analysis.
Animal Study:^{^[3]}	Animal Models Short bowel syndrome model mice (C57BL6/J; male) Dosages 0.1 mg/kg Administration i.p.

Cell Assay:^{^[4]}

Cell lines
Human Caco-2 intestinal epithelial cells
Concentrations
500 nM
Incubation Time
72 h
Method
Caco-2 cells (10 000 cells per 96-well plate) are plated in cell culture medium for 24 hours. The next day, the cells are incubated with 500nM teduglutidein serum-free medium for 72 hours. Then the cells are harvested for cell proliferation assay, bromodeoxyuridine incorporation assay and cell cycle analysis.

Animal Study:^{^[3]}

Animal Models
Short bowel syndrome model mice (C57BL6/J; male)
Dosages
0.1 mg/kg
Administration
i.p.

References

[4] Chaturvedi LS, et al. JAMA Surg. 2013 Nov;148(11):1037-42.

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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