TC-H 106

Catalog No.S6738 Batch:S673802

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Technical Data

Formula

C20H25N3O2

 

Molecular Weight 339.43 CAS No. 937039-45-7
Solubility (25°C)* In vitro DMSO 68 mg/mL (200.33 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description TC-H 106 (Pimelic Diphenylamide 106) is a slow, tight-binding inhibitor of class I histone deacetylases(HDAC) with Ki value of 148 nM, about 102 nM, 14 nM for HDAC1, HDAC2, HDAC3, respectively.
Targets
HDAC3 [1]
(Cell-free assay)
HDAC2 [1]
(Cell-free assay)
HDAC1 [1]
(Cell-free assay)
14 nM(Ki) ~102 nM(Ki) 148 nM(Ki)
In vitro

The ability of TC-H 106 to prolong histone acetylation has also been observed in cell culture. Cells treated with TC-H 106 (at a sub-EC10 concentration as determined with an MTS cell proliferation assay) have hyperacetylated histone H3. These cells do not lose significant amounts of acetylated histone H3 even after removal of the inhibitor for hours.[1].

Protocol (from reference)

Cell Assay:

[1]

  • Cell lines

    lymphoblastoid cell line

  • Concentrations

    2 μM

  • Incubation Time

    24 h

  • Method

    Cells are cultured in RPMI 1640 media with 10% fetal bovine serum and 10 mM HEPES, at 37 °C in 5% CO2. After the 5th split, the cells are treated with inhibitor TC-H 106 (2 μM) for 24 h, in culture medium. 24 h after treatment, the cells are washed twice with Hanks' balanced salts buffer to remove the inhibitor. A portion of the cell population is then harvested immediately after washing, and referred to as a time 0 point (24 h treatment point), and the rest of the cells are then re-cultured in cell culture media without added inhibitor. The re-cultured cells and controls are then harvested every hour for a total of 7 h.

Selleck's TC-H 106 has been cited by 4 publications

β1 integrin signaling governs necroptosis via the chromatin-remodeling factor CHD4 [ Cell Rep, 2023, 42(11):113322] PubMed: 37883227
PATJ inhibits histone deacetylase 7 to control tight junction formation and cell polarity [ Cell Mol Life Sci, 2023, 80(11):333] PubMed: 37878054
PATJ inhibits histone deacetylase 7 to control tight junction formation and cell polarity [ Cell Mol Life Sci, 2023, 80(11):333] PubMed: 37878054
Rb-E2F-HDAC Repressor Complexes Control Interferon-Induced Repression of Adenovirus To Promote Persistent Infection [ J Virol, 2022, e0044222] PubMed: 35546119

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.