Taurocholic acid sodium salt hydrate

Catalog No.S5130 Batch:S513001

Technical Data

Formula	C₂₆H₄₅NO₇S.xH₂O.Na
Molecular Weight	537.68 (anhydrous basis)	CAS No.	345909-26-4
Solubility (25°C)*	In vitro	DMSO	100 mg/mL


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Taurocholic acid (Sodium taurocholate hydrate), a bile salt formed in the liver, is the product of conjugation of cholic acid with taurine that is involved in the emulsification of lipids. Its sodium salt is the chief ingredient of the bile of carnivorous animals.
In vitro	The immunoregulation effect of TCA wasmediated through regulating both gene and protein expressions of TNF-α and IL-1β^[2].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines HTR-8/SVneo cells Concentrations 0, 10, 50 and 100 μM Incubation Time 6 h Method HTR-8/SVneo cells were sub-cultured in 6-well plates at a density of 2×10⁵ cells per well with complete culture medium overnight, and then exposed to different concentrations of TCA (0 μM, 10 μM, 50 μM and 100 μM) for 6 h. Cells were harvested using trypsin, washed with PBS, resuspended in 1 ml binding buffer, and stained with 5 ml Annexin V-FITC and 5 μl PI at room temperature for 10 min. FITC and PI fluorescence was analyzed by flow cytometry.
Animal Study:^{^[2]}	Animal Models Kunming mice Dosages 0.25 and 0.125 g/kg Administration oral

Cell Assay:^{^[1]}

Cell lines
HTR-8/SVneo cells
Concentrations
0, 10, 50 and 100 μM
Incubation Time
6 h
Method
HTR-8/SVneo cells were sub-cultured in 6-well plates at a density of 2×10⁵ cells per well with complete culture medium overnight, and then exposed to different concentrations of TCA (0 μM, 10 μM, 50 μM and 100 μM) for 6 h. Cells were harvested using trypsin, washed with PBS, resuspended in 1 ml binding buffer, and stained with 5 ml Annexin V-FITC and 5 μl PI at room temperature for 10 min. FITC and PI fluorescence was analyzed by flow cytometry.

Animal Study:^{^[2]}

Animal Models
Kunming mice
Dosages
0.25 and 0.125 g/kg
Administration
oral

References

Selleck's Taurocholic acid sodium salt hydrate has been cited by 2 publications

Discovery of senolytics using machine learning [ Nat Commun, 2023, 14(1):3445]	PubMed: 37301862
Discovery of senolytics using machine learning [ Nat Commun, 2023, 14(1):3445]	PubMed: 37301862

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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