Tannic acid

Catalog No.S3951 Batch:S395102

Technical Data

Formula	C₇₆H₅₂O₄₆
Molecular Weight	1701.2	CAS No.	1401-55-4
Solubility (25°C)*	In vitro	DMSO	94 mg/mL (55.25 mM)
		Water	94 mg/mL (55.25 mM)
		Ethanol	50 mg/mL (29.39 mM)
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

Tannic acid (Gallotannic acid), a polyphenolic compound, is a CXCL12/CXCR4 inhibitor with antiangiogenic, anti-inflammatory and antitumor activity.

Targets

CXCR4 ^[1]

In vitro

Tannic acid, at nontoxic concentrations, specifically inhibits CXCL12-induced human monocyte migration (IC50, 7.5 μg/ml) but does not inhibit CCL2-, CCL3-, CCL5-, formylmethionylleucylphenylalanine (fMLP)-, or C5a-induced migration. The compound markedly blocks CXCL12 binding to THP-1 cells (IC50, 0.36 μg/ml). Tannic acid also inhibits CXCL12-induced, but not epidermal growth factor-induced, migration of MDA 231 breast tumor cells. Additionally, 0.5 μg/ml of tannic acid selectively inhibits CXCL12-mediated, but not basic fibroblast growth factor- or endothelial cell growth supplement-mediated, bovine aorta endothelial cell capillary tube formation^[1].

In vivo

Given in drinking water, tannic acid enhances the survival rate of mice bearing syngeneic tumors^[1].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines THP-1 cells or BAEC cells Concentrations 0-100 μg/ml Incubation Time 24 h Method Freshly isolated human monocytes, THP-1 cells or BAEC cells, are plated into 96-microwell plates at 1 × 10⁵ cells/well in the presence of tannic acid at a final concentration of 100 μg/ml. Cell viability is determined by [3H]thymidine incorporation (THP-1 cells and BAEC cells) or trypan blue exclusion (human monocytes), 24 h after coincubation at 37°C in humidified air with 5% CO2.

Cell Assay:^{^[1]}

Cell lines
THP-1 cells or BAEC cells
Concentrations
0-100 μg/ml
Incubation Time
24 h
Method
Freshly isolated human monocytes, THP-1 cells or BAEC cells, are plated into 96-microwell plates at 1 × 10⁵ cells/well in the presence of tannic acid at a final concentration of 100 μg/ml. Cell viability is determined by [3H]thymidine incorporation (THP-1 cells and BAEC cells) or trypan blue exclusion (human monocytes), 24 h after coincubation at 37°C in humidified air with 5% CO2.

References

[1] Chen X, et al. Clin Cancer Res. 2003, 9(8):3115-23.

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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