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Technical Data
| Formula | C13H14N2.HCl |
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| Molecular Weight | 234.72 | CAS No. | 1684-40-8 | |
| Solubility (25°C)* | In vitro | DMSO | 46 mg/mL (195.97 mM) | |
| Water | 46 mg/mL (195.97 mM) | |||
| Ethanol | 46 mg/mL (195.97 mM) | |||
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* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.) |
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Preparing Stock Solutions
Biological Activity
| Description | Tacrine is a centrally acting anticholinesterase and indirect cholinergic agonist. Tacrine hydrochloride hydrate is an inhibitor of both acetyl (AChE) and butyryl-cholinestrase (BChE) with IC50s of 31 nM and 25.6 nM, respectively. | ||||
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| Targets |
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| In vitro | Tacrine hydrochloride is an inhibitor of three different hepatic microsomal cytochrome P-450 enzyme sub-families. Tacrine hydrochloride at 40 mg/mL, 80 mg/mL or 200 mg/mL inhibits 3-hydroxymethyl antipyrine (HMA) production by 17%, 24% and 41% and OHA production by 52%, 55% and 79%, respectively, in hepatic microsome. [1] Tacrine severely inhibits normal levels of secretion of soluble APP derivatives by cells into conditioned media in glial, fibroblast, pheochromocytoma (PC12), and neuroblastoma cells. Tacrine treatment does not alter the level of HSP-70 in cell extracts and tacrine affected mildly the secretion of PN-1 in neuroblastoma and PC12 cells. [2] Tacrine (1 μM) attenuates the neurotoxic effect of A beta(25-35) in rat PC12 cells. [3] |
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| In vivo | Tacrine (3 mg/kg, i.p.) prevents the avoidance impairment induced by 5 mg/kg amitriptyline on shuttle-box avoidance acquisition as well as on a previously learned avoidance response in mice. [4] Tacrine (5mg/kg) shows significant effects of the inhibition of brain AChE for more than 6 hours in the rat hippocampus. Tacrine (5mg/kg) increases acetylcholine concentration in the synaptic cleft of the hippocampus mostly through AChE inhibition in the rat hippocampus, and the maximum increases are observed at about 1.5 hours. [5] |
Protocol (from reference)
References
RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.
SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.
NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.