G10 (STING agonist-1)

Catalog No.S8954 Batch:S895401

Print

Technical Data

Formula

C21H16ClFN2O3S
Molecular Weight 430.88 CAS No. 702662-50-8
Solubility (25°C)* In vitro DMSO 63 mg/mL (146.21 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description G10 (STING agonist-1) is a novel human-specific STING agonist that triggers IFN regulatory factor 3 (IRF3)/ type I interferon (IFN)-associated transcription in human fibroblasts. STING agonist-1 (G10) potently reduces growth of Chikungunya virus (CHIKV) with IC90 of 8.01 μM and blocks replication of Alphavirus species Venezuelan Equine Encephalitis Virus (VEEV) with IC90 of 24.57 μM.
Targets
STING [1] IRF3/IFN [1] CHIKV [1]
(Cell-based assay)		 VEEV [1]
(Cell-based assay)
8.01 μM 24.57 μM
In vitro

G10 induces IFN/IRF3- but not NF-κB-dependent transcription in human fibroblasts. G10 elicits antiviral activity against New and Old World Alphaviruses. G10 induces STING-dependent synthesis and secretion of bioactive interferon. G10 triggers IRF3-dependent activity and induces innate antiviral mRNA expression in primary human cells.[1]
In vivo

Because G10-induced innate immune activation is not observed in murine cells, DMXAA (an analogs of G10, with similar physiological effects) is used in the commenly used mice model of CHIKV infection. STING agonism inhibits CHIKV replication in vivo.[1]

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    Human foreskin fibroblasts, Vero cells, BHK-21 cells, C6/36 cells, RAW264.7 cells, THP1-ISG-Lucia cells, Human peripheral blood mononuclear cells

  • Concentrations

    6.25-200 μM, 100 μM

  • Incubation Time

    7 h, 18 h

  • Method

    --

Selleck's G10 (STING agonist-1) has been cited by 1 publication

Lats2 deficiency protects the heart against myocardial infarction by reducing inflammation and inhibiting mitochondrial fission and STING/p65 signaling [ Int J Biol Sci, 2023, 19(11):3428-3440] PubMed: 37497006

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.