STAT5-IN-1

Catalog No.S6784 Batch:S678403

Technical Data

Formula

C₁₆H₁₁N₃O₃

Molecular Weight

293.28

CAS No.

285986-31-4

Solubility (25°C)*

In vitro

DMSO

15 mg/mL (51.14 mM)

Water

Insoluble

Ethanol

Insoluble

In vivo (Add solvents to the product individually and in order)

Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	0.75mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 15 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMSO 1 Corn oil	0.75mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 15 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

STAT5-IN-1 is a potent and selective STAT5 inhibitor with IC50 of 47 μM for STAT5β isoform.

Targets

STAT5β ^[1]
(Cell-free assay)

47 μM

In vitro

STAT5-IN-1, the STAT5 phosphorylation specific inhibitor, selectively blocks the STAT5 signaling and decreases the percentage of CD4+CD25+Foxp3+ Tregs after SEC2 stimulation on murine splenocytes.^[2]

In vivo

STAT5-IN-1, a STAT5 inhibitor, significantly attenuates atherosclerosis in ApoE^-/- mice induced by HFD via decreasing inflammation.^[3]

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines murine splenocytes Concentrations 0.47 mM Incubation Time 3 days Method Fresh murine splenocytes seeded in 24-well flat-bottom plates are treated with each inhibitor at final concentrations of 10 μM LY294002, 10 mM Trigonelline, 0.47 mM STAT5-IN-1, 50 μM SB431542, and 1 μM SIS3, or treated with 10 μg/mL anti-IL-2 neutralizing antibody, respectively, then cells are stimulated with 10⁴ ng/mL SEC2 for 3 days in a humidified incubator at 37 ℃ with 5% CO₂.
Animal Study:^{^[3]}	Animal Models ApoE^-/- mice Dosages 10 mg/kg Administration p.o.

Cell Assay:^{^[2]}

Cell lines
murine splenocytes
Concentrations
0.47 mM
Incubation Time
3 days
Method
Fresh murine splenocytes seeded in 24-well flat-bottom plates are treated with each inhibitor at final concentrations of 10 μM LY294002, 10 mM Trigonelline, 0.47 mM STAT5-IN-1, 50 μM SB431542, and 1 μM SIS3, or treated with 10 μg/mL anti-IL-2 neutralizing antibody, respectively, then cells are stimulated with 10⁴ ng/mL SEC2 for 3 days in a humidified incubator at 37 ℃ with 5% CO₂.

Animal Study:^{^[3]}

Animal Models
ApoE^-/- mice
Dosages
10 mg/kg
Administration
p.o.

References

Selleck's STAT5-IN-1 has been cited by 14 publications

Sustained AhR activity programs memory fate of early effector CD8+ T cells [ Proc Natl Acad Sci U S A, 2024, 121(11):e2317658121]	PubMed: 38437537
Macrophage re-programming by JAK inhibitors relies on MAFB [ Cell Mol Life Sci, 2024, 81(1):152]	PubMed: 38528207
hFcγRIIa: a double-edged sword in osteoclastogenesis and bone balance in transgenic mice [ Front Immunol, 2024, 15:1425670]	PubMed: 39281679
Hedgehog receptors exert immune-surveillance roles in the epidermis across species [ Cell Rep, 2023, 42(8):112929]	PubMed: 37527037
CRISPR/Cas9-engineering of HMC-1.2 cells renders a human mast cell line with a single D816V-KIT mutation: An improved preclinical model for research on mastocytosis [ Front Immunol, 2023, 14:1078958]	PubMed: 37025992
GM-CSF-activated STAT5A regulates macrophage functions and inflammation in atherosclerosis [ Front Immunol, 2023, 10.3389/fimmu.2023.1165306]	PubMed: 37920458
Vitamin A- and D-Deficient Diets Disrupt Intestinal Antimicrobial Peptide Defense Involving Wnt and STAT5 Signaling Pathways in Mice [ Nutrients, 2023, 15(2)376]	PubMed: 36678247
Human α-Defensin 51-9 and Human β-Defensin 2 Improve Metabolic Parameters and Gut Barrier Function in Mice Fed a Western-Style Diet [ Int J Mol Sci, 2023, 24(18)13878]	PubMed: 37762180
BCL6 inhibition ameliorates ruxolitinib resistance in CRLF2-rearranged acute lymphoblastic leukemia [ Haematologica, 2022, 10.3324/haematol.2022.280879]	PubMed: 36005560
Regulation of Il-2 on the expression of granzyme B- and perforin-like genes and its functional implication in grass carp peripheral blood neutrophils [ Fish Shellfish Immunol, 2022, 124:472-479]	PubMed: 35483596

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.