STAT5-IN-1

Catalog No.S6784 Batch:S678402

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Technical Data

Formula

C16H11N3O3
Molecular Weight 293.28 CAS No. 285986-31-4
Solubility (25°C)* In vitro DMSO 20 mg/mL (68.19 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description STAT5-IN-1 is a potent and selective STAT5 inhibitor with IC50 of 47 μM for STAT5β isoform.
Targets
STAT5β [1]
(Cell-free assay)
47 μM
In vitro

STAT5-IN-1, the STAT5 phosphorylation specific inhibitor, selectively blocks the STAT5 signaling and decreases the percentage of CD4+CD25+Foxp3+ Tregs after SEC2 stimulation on murine splenocytes.[2]
In vivo

STAT5-IN-1, a STAT5 inhibitor, significantly attenuates atherosclerosis in ApoE-/- mice induced by HFD via decreasing inflammation.[3]

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    murine splenocytes

  • Concentrations

    0.47 mM

  • Incubation Time

    3 days

  • Method

    Fresh murine splenocytes seeded in 24-well flat-bottom plates are treated with each inhibitor at final concentrations of 10 μM LY294002, 10 mM Trigonelline, 0.47 mM STAT5-IN-1, 50 μM SB431542, and 1 μM SIS3, or treated with 10 μg/mL anti-IL-2 neutralizing antibody, respectively, then cells are stimulated with 104 ng/mL SEC2 for 3 days in a humidified incubator at 37 ℃ with 5% CO2.
Animal Study:

[3]
  • Animal Models

    ApoE-/- mice

  • Dosages

    10 mg/kg

  • Administration

    p.o.

Selleck's STAT5-IN-1 has been cited by 14 publications

Sustained AhR activity programs memory fate of early effector CD8+ T cells [ Proc Natl Acad Sci U S A, 2024, 121(11):e2317658121] PubMed: 38437537
Macrophage re-programming by JAK inhibitors relies on MAFB [ Cell Mol Life Sci, 2024, 81(1):152] PubMed: 38528207
hFcγRIIa: a double-edged sword in osteoclastogenesis and bone balance in transgenic mice [ Front Immunol, 2024, 15:1425670] PubMed: 39281679
Hedgehog receptors exert immune-surveillance roles in the epidermis across species [ Cell Rep, 2023, 42(8):112929] PubMed: 37527037
CRISPR/Cas9-engineering of HMC-1.2 cells renders a human mast cell line with a single D816V-KIT mutation: An improved preclinical model for research on mastocytosis [ Front Immunol, 2023, 14:1078958] PubMed: 37025992
GM-CSF-activated STAT5A regulates macrophage functions and inflammation in atherosclerosis [ Front Immunol, 2023, 10.3389/fimmu.2023.1165306] PubMed: 37920458
Vitamin A- and D-Deficient Diets Disrupt Intestinal Antimicrobial Peptide Defense Involving Wnt and STAT5 Signaling Pathways in Mice [ Nutrients, 2023, 15(2)376] PubMed: 36678247
Human α-Defensin 51-9 and Human β-Defensin 2 Improve Metabolic Parameters and Gut Barrier Function in Mice Fed a Western-Style Diet [ Int J Mol Sci, 2023, 24(18)13878] PubMed: 37762180
BCL6 inhibition ameliorates ruxolitinib resistance in CRLF2-rearranged acute lymphoblastic leukemia [ Haematologica, 2022, 10.3324/haematol.2022.280879] PubMed: 36005560
Regulation of Il-2 on the expression of granzyme B- and perforin-like genes and its functional implication in grass carp peripheral blood neutrophils [ Fish Shellfish Immunol, 2022, 124:472-479] PubMed: 35483596

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.