SSE15206

Catalog No.S8789 Batch:S878901

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Technical Data

Formula

C19H21N3O3S
Molecular Weight 371.45 CAS No. 1370046-40-4
Solubility (25°C)* In vitro  
 
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Biological Activity

Description SSE15206 is a tubulin polymerization inhibitor that has potent antiproliferative activities in cancer cell lines of different origins and overcomes resistance to microtubule-targeting agents.
Targets
tubulin polymerization [1]
In vitro

Treatment of cells with SSE15206 causes aberrant mitosis resulting in G2/M arrest due to incomplete spindle formation, a phenotype often associated with drugs that interfere with microtubule dynamics. SSE15206 inhibits microtubule polymerization both in biochemical and cellular assays by binding to colchicine site in tubulin. Prolonged treatment of cells with SSE15206 results in apoptotic cell death accompanied by p53 induction[1].

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    HCT116 cells

  • Concentrations

    0.5 μM, 1 μM and 2 μM

  • Incubation Time

    4, 8, 12, 24 and 36 hours

  • Method

    HCT116 cells are treated with 0.5 μM, 1 μM and 2 μM SSE15206 for 4 and 8 hours. Phosphorylation of histone H3 (S10) and P-MPM2 is determined by western blotting; total-histone H3 and GAPDH are used as loading controls; Or HCT116 cells are treated with 1 μM SSE15206 for 4, 8, 12, 24 and 36 hours and are analyzed through FACS following treatment.

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.