SR9243

Catalog No.S7934 Batch:S793402

Technical Data

Formula	C₃₁H₃₂BrNO₄S₂
Molecular Weight	626.62	CAS No.	1613028-81-1
Solubility (25°C)*	In vitro	DMSO	33 mg/mL (52.66 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

SR9243 is a potent and selective LXR inverse agonist.

Targets

LXR ^[1]

In vitro

In HEK293 cells expressing LXRs, SR9243 inhibits LXR activation by enhancing LXR-corepressor recruitment. In a variety of cancer cell types, SR9243 reduces cancer cell viability, induces apoptotic cell death, and sensitizes cancer cells to chemotherapeutic treatments. ^[1]

In vivo

In Ob/Ob mice fed a high-fat diet, SR9243 (60 mg/kg, i.p.) inhibits LXR-dependent lipogenic enzyme gene expression and tumor growth. ^[1]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines DU-145, SW620, HOP-62, PC3, HT-29, and NCI-H23 cancer cells Concentrations 100 nM Incubation Time 96 h Method Cells are cultured in 96 well plates and treated with designated amounts of SR9243 for 96 hr in media containing 1% FBS and antibiotics. Cell-viability is assessed using the Cell-titre 96 kit according to the manufacturer’s guidelines. Cell culture media is supplemented with oleate, stearate and palmitate dissolved in methanol to a concentration of 25 mM. 25 mM stocks are then diluted 10 fold in PBS containing 0.9% BSA. Lipid stocks (100X) are stored at −20° until needed.
Animal Study:^{^[1]}	Animal Models Mice bearing SW620, DU-145, or LLC tumors Dosages 60 mg/kg, once daily Administration i.p.

Cell Assay:^{^[1]}

Cell lines
DU-145, SW620, HOP-62, PC3, HT-29, and NCI-H23 cancer cells
Concentrations
100 nM
Incubation Time
96 h
Method
Cells are cultured in 96 well plates and treated with designated amounts of SR9243 for 96 hr in media containing 1% FBS and antibiotics. Cell-viability is assessed using the Cell-titre 96 kit according to the manufacturer’s guidelines. Cell culture media is supplemented with oleate, stearate and palmitate dissolved in methanol to a concentration of 25 mM. 25 mM stocks are then diluted 10 fold in PBS containing 0.9% BSA. Lipid stocks (100X) are stored at −20° until needed.

Animal Study:^{^[1]}

Animal Models
Mice bearing SW620, DU-145, or LLC tumors
Dosages
60 mg/kg, once daily
Administration
i.p.

References

[1] Flaveny CA, et al. Cancer Cell. 2015, 28(1), 42-56

Selleck's SR9243 has been cited by 2 publications

Myeloid-Derived Suppressive Cells Deficient in Liver X Receptor α Protected From Autoimmune Hepatitis [ Front Immunol, 2021, 12:732102]	PubMed: 34512667
Paraoxonase 1-treated oxLDL promotes cholesterol efflux from macrophages by stimulating the PPARγ-LXRα-ABCA1 pathway. [Ikhlef S, et al. FEBS Lett, 2016, 590(11):1614-29]	PubMed: 27148853

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.