Spiramycin

Catalog No.S4082 Batch:S408202

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Technical Data

Formula

C43H74N2O14
Molecular Weight 843.05 CAS No. 8025-81-8
Solubility (25°C)* In vitro DMSO 100 mg/mL (118.61 mM)
Ethanol 100 mg/mL (118.61 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5% DMSO 95% Corn oil
1.25mg/ml Taking the 1 mL working solution as an example, add 50 μL of 25 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
5.0mg/ml Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Spiramycin (Formacidine) is a 16-membered ring macrolide (antibiotic).
In vitro Spiramycin reduces the hygromycin A protections of nucleotides in 23 S rRNA. [1] Spiramycin, a 16-membered macrolide, inhibits translocation by binding to bacterial 50S ribosomal subunits with an apparent 1:1 stoichiometry. Spiramycin acts primarily by stimulating the dissociation of peptidyl-tRNA from ribosomes during translocation. [2] Spiramycin at doses sufficient to inhibit protein synthesis in wild-type cells but not sufficient to kill either mutant or wild-type cells at the permissive temperature (30 degrees C). Spiramycin inhibits protein synthesis by stimulating the dissociation of peptidyl-tRNA from ribosomes. [3] Spiramycin shows dose-related inhibition of the proliferative response of PHA and PWM stimulated human mononuclear leucocytes (MNL). Spiramycin also induces a decrease in tritiated uridine (3H-UdR) uptake, which suggests that Spiramycin interferes with an early event in the cell cycle. [4] Spiramycin and, to a lesser extent, erythromycin increases total IL-6 production without affecting IL-1 alpha, IL-1 beta, or tumor necrosis factor alpha production in human monocytes stimulated with lipopolysaccharide. [5] Spiramycin shows good antimicrobial activity against species of Prevotella, Eubacterium, Peptostreptococcus, Bacteroides and Porphyromonas, and the effect is enhanced by the addition of Metronidazole. [6]

Protocol (from reference)

Selleck's Spiramycin has been cited by 1 publication

Solution conformations explain the chameleonic behaviour of macrocyclic drugs. [ Chemistry, 2020, 10.1002/chem.201905599] PubMed: 32027758

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.